Eight-component two-fermion equations

An eight-component formalism is proposed for the relativistic two-fermion problem. In QED, it extends the applicability of the Dirac equation with hyperfine interaction to the positronium case. The use of exact relativistic two-body kinematics entails a CP-invariant spectrum which is symmetric in the total cms energy. It allows the extension of recent \alpha^6 recoil corrections to the positronium case, and implies new recoil corrections to the fine and hyperfine structures and to the Bethe logarithm.Comment: Revtex, accepted for publication in Phys. Rev.

The Organophosphate Chlorpyrifos Interferes with the Responses to 17β-Estradiol in the Digestive Gland of the Marine Mussel Mytilus galloprovincialis

BACKGROUND: Many pesticides have been shown to act as endocrine disrupters. Although the potencies of currently used pesticides as hormone agonists/antagonists are low compared with those of natural ligands, their ability to act via multiple mechanisms might enhance the biological effect. The organophosphate Chlorpyrifos (CHP) has been shown to be weakly estrogenic and cause adverse neurodevelopmental effects in mammals. However, no information is available on the endocrine effects of CHP in aquatic organisms. In the digestive gland of the bivalve Mytilus galloprovincialis, a target tissue of both estrogens and pesticides, the possible effects of CHP on the responses to the natural estrogen 17β-estradiol (E(2)) were investigated. METHODOLOGY/PRINCIPAL FINDINGS: Mussels were exposed to CHP (4.5 mg/l, 72 hrs) and subsequently injected with E(2) (6.75 ng/g dw). Responses were evaluated in CHP, E(2) and CHP/E(2) treatment groups at 24 h p.i. by a biomarker/transcriptomic approach. CHP and E(2) induced additive, synergistic, and antagonistic effects on lysosomal biomarkers (lysosomal membrane stability, lysosome/cytoplasm volume ratio, lipofuscin and neutral lipid accumulation). Additive and synergistic effects were also observed on the expression of estrogen-responsive genes (GSTπ, catalase, 5-HTR) evaluated by RT-Q-PCR. The use of a 1.7K cDNA Mytilus microarray showed that CHP, E(2) and CHP/E(2), induced 81, 44, and 65 Differentially Expressed Genes (DEGs), respectively. 24 genes were exclusively shared between CHP and CHP/E(2), only 2 genes between E(2) and CHP/E(2). Moreover, 36 genes were uniquely modulated by CHP/E(2). Gene ontology annotation was used to elucidate the putative mechanisms involved in the responses elicited by different treatments. CONCLUSIONS: The results show complex interactions between CHP and E(2) in the digestive gland, indicating that the combination of certain pesticides and hormones may give rise to unexpected effects at the molecular/cellular level. Overall, these data demonstrate that CHP can interfere with the mussel responses to natural estrogens

The Impact of Long-Term Exposure to Space Environment on Adult Mammalian Organisms: A Study on Mouse Thyroid and Testis

Hormonal changes in humans during spaceflight have been demonstrated but the underlying mechanisms are still unknown. To clarify this point thyroid and testis/epididymis, both regulated by anterior pituitary gland, have been analyzed on long-term space-exposed male C57BL/10 mice, either wild type or pleiotrophin transgenic, overexpressing osteoblast stimulating factor-1. Glands were submitted to morphological and functional analysis

Pleiotropic effects of hexavalent chromium (CrVI) in Mytilus galloprovincialis digestive gland.

Hexavalent Chromium Cr(VI) is an important contaminant considered as a model oxidative toxicant released from both domestic and industrial effluents, and represents the predominant chemical form of the metal in aquatic ecosystems. On the other hand, in mammals the reduced form Cr(III) is considered an essential microelement, involved in regulation of lipid and carbohydrate metabolism; moreover, recent evidence suggests that Cr may have endocrine effects. In this work, the effects of Cr(VI) were investigated in the digestive gland of the marine bivalve Mytilus Keywords: galloprovincialis. Mussels were exposed to 0.1–1–10–100 lg Cr(VI) LÀ1 animalÀ1 for 96 h. At 100 lg LÀ1, a Cr(VI) large increase in total Cr tissue content was observed; in these conditions, the lysosomal membranes Bivalves were completely destabilized, whereas other lysosomal biomarkers (neutral lipids-NL and lipofuscin- Digestive gland LF), as well as different enzyme activities and gene expression were unaffected, this indicating severe Lysosomal biomarkers stress conditions in the tissue. On the other hand, at lower concentrations, changes in other histochem- Glycolytic enzymes ical, biochemical and molecular endpoints were observed. In particular, at both 1 and 10 lg LÀ1, lyso- Gene expression somal destabilization was associated with significant NL and LF accumulation; however, no changes in catalase and GSH transferase (GST) activities were observed. At the same concentrations, GSSG reductase (GSR) activity was significantly increased, this probably reflecting the recycling of GSSG produced in the GSH-mediated intracellular reduction of Cr(VI). Increased activities of the key glycolytic enzymes PFK (phosphofructokinase) and PK (pyruvate kinase) were also observed, indicating that Cr(VI) could affect carbohydrate metabolism. Cr(VI) induced downregulation or no effects on the expression of metallothio- neins MT10 and MT20, except for an increase in MT20 transcription in males. Moreover, significant up- regulation of the Mytilus estrogen receptor MeER2 and serotonin receptor (5-HTR) were observed in both sexes. The results demonstrate that exposure to Cr(VI) in the low ppb range did not result in strong toxicity or oxidative stress conditions in mussel digestive gland. On the other hand, our data support the hypothesis that low concentrations of the metal can exert pleiotropic effects on mussel physiology, from modulation of lipid and carbohydrate metabolism, to effects on the expression of estrogen-responsive genes

The impact of long-term exposure to Space environment on adult mouse testis

The presence of 3- and 17 \u3b2 HSD, the mRNA expression of FSH receptor (FSHR), LH receptor (LHR) and androgen receptor (AR), interleukin-1\u3b2 (IL-1\u3b2) were evaluated, by quantitative RT-PCR, in the testis of mice (C57BL/10) under different gravity conditions (0 g: MDS, 91 days in International Space Station, ISS, and 1 g: ground control) either wild type or pleiotrophin transgenic (overexpressing osteoblast stimulating factor-1). Histological examination of the testes of the control groups revealed regularly configured seminiferous tubules. The testes from spaceflown animals show disorganization, sloughing of cells in the lumen, separation of germ cells from the basal laminae and vacuolation of the germinal epithelium. The interstitial tissue displayed inflammatory exudates. No great differences are observed between Wild (WT) and Transgenic (TG) mice. Immunihistochemistry for 3\u3b2-HSD and 17\u3b2-HSD shows similar localization in Leydig cells in all animals. Therefore the immunoreactions of ISS (WT and TG) decreases. HSP70 immunohistofluorescence was localized in the interstitial tissue in flown samples. Transcription of the studied genes was comparable in the ground controls, with slightly higher levels of mRNA transcripts in Transgenic mice with respect to WT. Microgravity did not affect expression of LHR in both samples, whereas an increase in FSHR was observed in WT. On the other hand, higher levels of mRNA transcripts for AR observed in flown testes (WT and TG). Moreover, a particularly high expression of IL-1\u3b2 was observed in samples from ISS

Effects of sublethal, environmentally relevant concentrations of hexavalent chromium in the gills of Mytilus galloprovincialis

Hexavalent chromium Cr(VI) is an important contaminant released from both domestic and industrial effluents, and represents the predominant chemical form of the metal in aquatic ecosystems. In the marine bivalve Mytilus galloprovincialis exposure to non-toxic, environmentally relevant concentrations of Cr(VI) was shown to modulate functional parameters and gene expression in both the digestive gland and hemocytes. In this work, the effects of exposure to Cr(VI) (0.1–1–10 g L−1 animal−1 for 96 h) in mussel gills were investigated. Gill morphology and immunolocalization of GSH-transferase (GST), of components involved in cholinergic (AChE and ChAT), adrenergic (TH) and serotoninergic (5-HT3 receptor) systems, regulating gill motility, were evaluated. Total glutathione content, activities of GSH-related enzymes (glutathione reductase – GSR, GST), of catalase, and of key glycolytic enzymes (phosphofructokinase – PFK and pyruvate kinase – PK) were determined. Moreover, mRNA expression of selected Mytilus genes (GST-, metallothionein isoforms MT10 and MT20, HSP70 and 5-HT receptor) was assessed by RT-q-PCR. Cr(VI) exposure induced progressive changes in gill morphology and in immunoreactivity to components involved in neurotransmission that were particularly evident at the highest concentration tested, and associated with large metal accumulation. Cr(VI) increased the activities of GST and GSR, and total glutathione content to a different extent at different metal concentrations, this suggesting Cr(VI) detoxication/reduction at the site of metal entry. Cr(VI) exposure also increased the activity of glycolytic enzymes, indicating modulation of carbohydrate metabolism. Significant changes in transcription of different genes were observed. In particular, the mRNA level for the 5-HTR was increased, whereas both decreases and increases were observed for GST-, MT10, MT20 and HSP70 mRNAs, showing sex- and concentration-related differences. The results demonstrate that Cr(VI) significantly affected functional and molecular parameters in mussel gills, and indicate that this tissue represents the major target of exposure to environmentally relevant concentrations of the meta