Effect of CryIIa transgenic chickpeas to Helicoverpa armigera larval parasitoid, Campoletis chlorideae

The current experiments were conducted to evaluate the effect of transgenic chickpea lines expressing CryIIa to C. chlorideae under laboratory conditions. There was a significant reduction in cocoon formation and adult emergence of C. chlorideae reared on H. armigera larvae fed on the leaves of transgenic chickpea before and after parasitisation. The larval period was prolonged and was a significant difference between the transgenic and nontransgenic chickpea lines was observed. Although the pupal period of the parasitoid was prolonged, there were no significant differences between the transgenic and nontransgenic chickpea lines. The adverse effects of transgenic chickpea lines on cocoon formation and adult emergence of C.chlorideae were largely due to the early mortality of H.armigera larvae, but there was no direct toxicity of Bt toxin protein to C. chlorideae. The amount of CryIIa protein transferred from leaves to the non-target insects and natural enemies were negligible

Evaluation of Cry IIa transgenic chickpea lines for resistance to Helicoverpa armigera (Hubner) using detached leaf assay

Studies were conducted to evaluate transgenic chickpea lines encoding Cry IIa for resistance to Helicoverpa armigera. Significantly lower leaf damage was noticed in transgenic chickpea lines when compared to non-transgenic lines. Significant reduction in larval survival and weight gain were observed when H. armigera were fed on transgenic lines under laboratory conditions. Across the seasons (2011-12 and 2012-13), the transgenic chickpea lines BS5A.2(T2) 19-1P2 and BS5A.2(T2) 19-2P1 showed enhanced levels of resistance to H. armigera

Direct effect of CryIIa transgenic chickpea on coccinellid, Cheilomenes sexmaculatus (Fabricius)

The experiments were conducted during 2012-2014 at ICRISAT, Hyderabad to study the direct effects of transgenic chickpea lines on coccinellid beetle, Cheilomenes sexmaculatus (Fabricius). The direct effects on coccinellids were greater when fed on 0.1% Bt intoxicated diet, followed by diets with 0.05% and 0.02% Bt. The survival and development of coccinellid grubs were slightly affected when reared on aphids fed on diets with different concentrations (0.02%, 0.05% and 0.1%) of transgenic chickpea leaf powder. The coccinellids fed on diets with 0.05% BS5A.2(T2) 19-3P1 leaf powder showed a marginal reduction in survival and development as compared to that on other transgenic lines

Influence of Nano-/Microfiller Addition on Mechanical and Morphological Performance of Kenaf/Glass Fibre-Reinforced Hybrid Composites

Natural-based composite’s progress as carriers has revealed many benefits in biomedicine, notably in the construction field, synthetic biology, and genetic engineering. Compared to analogous composites without nanoparticles, incorporating nanoparticles into polymeric materials improved architectural performance, physiological connections, and ecological features. The major goal of the current investigation is to determine the impact of nano-/micro-TiO2 on the mechanical characteristics of kenaf/glass/epoxy hybrids. The samples have been created using a hand layup method and a variety of filler loading and stacking sequences. The addition of nano-/microfiller significantly improved the mechanical performance of the epoxy/hybrid composite material. It was discovered that nanofiller-added composite materials fared better when composites were compared to and without microfilter-added composites. SEM was used to investigate the microstructure of the interfaces to ensure a good understanding of interfacial adherence between the reinforcement and their matrix. Compared to pure epoxy resin, the 15 wt% of microfiller additions of glass-kenaf-kenaf-glass type composites exhibit a 39.48% improvement in tensile and a 42.88% improvement in flexural. Similarly, 5 wt% nanofiller addition reveals a 44.214% improvement in tensile and a 50.50% improvement in flexural

Protein kinases orchestrate cell cycle regulators in differentiating BeWo choriocarcinoma cells

Abstract Choriocarcinoma, a trophoblastic neoplasia, occurs in women as an incidence of abnormal pregnancy. BeWo choriocarcinoma cells derived from the abnormal placentation are a suitable model system to study the factors associated with differentiation, invasion and other cellular events as an alternative to clinical samples. Many protein kinases orchestrate the complex events of cell cycle and in case of malignancy such regulators are found to be mutated. In the present study, BeWo cells treated with forskolin (Fo) and phorbol 12-myristate 13-acetate (PMA) were used to study the role of PKA (protein kinase A) and PKC (protein kinase C), respectively, on the expression pattern of differentiation-related genes, membrane markers, PKC isoforms and cell cycle regulators. The effect of Fo and PMA on the cell proliferation was assessed. Progressive induction of alkaline phosphatase level and formation of multinucleated differentiated cells were observed in the cells treated with Fo. Exposure of cells to Fo and PMA induced the mRNA transcripts of α-hCG, β-hCG and endoglin and down-regulates E-cadherin at mRNA and protein levels. Synergistic levels of both up- and down-regulated genes/proteins were observed when cells were treated with the combination of Fo and PMA. The mRNA levels of cyclin D1, cyclin E1, p21, Rb, p53, caspase-3 and caspase-8 decreased gradually during differentiation. Fo significantly inhibited the protein levels of PCNA, Rb, PKC-α and PMA stimulated mRNA expression of PKC-ε and PKC-δ. Further, failure in the activation of essential components of the cell cycle machinery caused G2/M phase arrest in differentiating BeWo cells

Monophasic synovial sarcoma of the pharynx: a case report

Synovial sarcomas are a rare form of soft tissue sarcomas. We present a case of a 62 year-old male presenting with a left thyroid lump initially though to be a thyroid adenoma but subsequently diagnosed as a monophasic synovial sarcoma of the pharynx. We discuss the diagnosis and treatment of this case

Frequency of ubiquitin and FUS-positive, TDP-43-negative frontotemporal lobar degeneration

Frontotemporal lobar degeneration (FTLD) is a clinically, genetically and pathologically heterogeneous disorder. Within FTLD with ubiquitin-positive inclusions (FTLD-U), a new pathological subtype named FTLD-FUS was recently found with fused in sarcoma (FUS) positive, TDP-43-negative inclusions, and striking atrophy of the caudate nucleus. The aim of this study was to determine the frequency of FTLD-FUS in our pathological FTLD series, and to describe the clinical, neuroimaging and neuropathological features of FTLD-FUS, especially caudate atrophy. Demographic and clinical data collected prospectively from 387 patients with frontotemporal dementia (FTD) yielded 74 brain specimens. Immunostaining was carried out using a panel of antibodies, including AT-8, ubiquitin, p62, FUS, and TDP-43. Cortical and caudate atrophy on MRI (n = 136) was rated as normal, mild-moderate or severe. Of the 37 FTLD-U cases, 33 were reclassified as FTLD-TDP and four (0.11, 95%: 0.00–0.21) as FTLD-FUS, with ubiquitin and FUS-positive, p62 and TDP-43-negative neuronal intranuclear inclusions (NII). All four FTLD-FUS cases had a negative family history, behavioural variant FTD (bvFTD), and three had an age at onset ≤40 years. MRI revealed mild-moderate or severe caudate atrophy in all, with a mean duration from onset till MRI of 63 months (range 16–119 months). In our total clinical FTD cohort, we found 11 patients (0.03; 95% CI: 0.01–0.05) with bvFTD, negative family history, and age at onset ≤40 years. Caudate atrophy was present in 10 out of 136 MRIs, and included all four FUS-cases. The newly identified FTLD-FUS has a frequency of 11% in FTLD-U, and an estimated frequency of three percent in our clinical FTD cohort. The existence of this pathological subtype can be predicted with reasonable certainty by age at onset ≤40 years, negative family history, bvFTD and caudate atrophy on MRI