Core promoter short tandem repeats as evolutionary switch codes for primate speciation

Alteration in gene expression levels underlies many of the phenotypic differences across species. Because of their highly mutable nature, proximity to the +1 transcription start site (TSS), and the emerging evidence of functional impact on gene expression, core promoter short tandem repeats (STRs) may be considered an ideal source of variation across species. In a genome-scale analysis of the entire Homo sapiens protein-coding genes, we have previously identified core promoters with at least one STR of ≥6-repeats, with possible selective advantage in this species. In the current study, we performed reverse analysis of the entire Homo sapiens orthologous genes in mouse in the Ensembl database, in order to identify conserved STRs that have shrunk as an evolutionary advantage to humans. Two protocols were used to minimize ascertainment bias. Firstly, two species sharing a more recent ancestor with Homo sapiens (i.e. Pan troglodytes and Gorilla gorilla gorilla) were also included in the study. Secondly, four non-primate species encompassing the major orders across Mammals, including Scandentia, Laurasiatheria, Afrotheria, and Xenarthra were analyzed as out-groups. We introduce STR evolutionary events specifically identical in primates (i.e. Homo sapiens, Pan troglodytes, and Gorilla gorilla gorilla) vs. non-primate out-groups. The average frequency of the identically shared STR motifs across those primates ranged between 0.00005 and 0.06. The identified genes are involved in important evolutionary and developmental processes, such as normal craniofacial development (TFAP2B), regulation of cell shape (PALMD), learning and long-term memory (RGS14), nervous system development (GFRA2), embryonic limb morphogenesis (PBX2), and forebrain development (APAF1). We provide evidence of core promoter STRs as evolutionary switch codes for primate speciation, and the first instance of identity-by-descent for those motifs at the interspecies level. © 2014 Wiley Periodicals, Inc

Establishment of Space Syntax to Read Urban Road Network; the Case of Sari, Iran

Cities have permanent changes as a living organism, where the transformation required in designing a solution for structural and social demands achieving safe and healthy human contacts. Some scholars divide city sustainable development toward two main views, as the building's set are connected by a space, on the other hand human social actions are linked by urban network interaction. The aim of this paper is to study on urban road network by the establishment of space syntax logic, this issue is divided into two main parts, as a first part, the study on already existing and the second part is bringing the new suggestions to a more qualified urban road network. Sari city of Iran is selected for this Case Study

The performance of a cable-stayed bridge pylon under close-range blast loads

Recent bridge collapses have raised an awareness of, and a concern for, the safety and robustness of bridges subjected to blast loading scenarios. The incident pressure generated by the explosion can cause severe structural damage and a loss of critical structural members, resulting in partial collapse of the bridge. Previously, most relevant research effort has been devoted to understanding the response of buildings under blast loading and to develop guidelines to increase the resistance of such structures, while relatively little research attention has been focused on bridge structures. Recent advancements in numerical methods have enabled the viable and cost-effective simulation of complicated blast scenarios, and hence these methods provide a useful reference for safeguarding design and assessment of critical infrastructure. To reduce the computational costs, previous studies on long span bridges under blast loads typically take advantage of sub-structuring techniques, in which only part of the structure is modelled. However, such oversimplifications can lead to erroneous results. Accordingly, this study is an attempt to simulate the dynamic response of an entire cable-stayed bridge subjected to blast loading based on best practice techniques obtained from the literature. The response of a steel bridge, designed according to the minimum requirements of the Australian Standard AS5100, is investigated when subjected to blast loads ranging from small to large explosions at different positions above the deck using numerical simulations. In addition, the potential effects of blast loads on different structural components (i.e. the deck and pylons) are discussed and possible blast mitigation strategies such as the application of FRP and optimization of the geometry of the pylons are investigated

Identification and Prioritization of Energy Consumption Optimization Strategies in the Building Industry Using the Hybrid SWARA-BIM Model

Energy consumption in buildings has become one of the most critical problems in all countries and principles of sustainability suggest that a satisfactory solution must be found to reduce energy consumption. This study aims to identify and prioritize energy consumption optimization strategies in buildings. Data collection consists of gather-ing primary data from the existing literature and secondary data from interviews, questionnaires, and simulations through building information modeling (BIM) tools. Twenty-nine strategies were identified and categorized into five groups according to their nature and ranked using one of the multiple criteria decision-making (MCDM) methods called the step-wise weight assessment ratio analysis (SWARA). A case study building in Shiraz, Iran, was simulated using BIM software, and the energy saving potential of the highest ranked strategies were obtained. According to the results, significant contributors to the energy consumption optimization were “Using renewable energy resources,” “Using efficient insulation,” and “Using suitable materials,” providing 100%, 35%, and 23% efficacy, respectively. The results obtained from this study can inform the building industry’s key stakeholders regarding the best strategies to apply in order to reduce energy consumption and improve sustainability in the construction industry

Study of chemical and physical behavior cotton fabrics treated by corona discharge after several laundering

Cotton fabric because of finishing process has shrinkage behavior after laundering and for improving this problem, chemical agents utilize for anti-shrinkage. This temporary finishing can reduce shrinkagebut has many environmental problems. Corona discharge treatment has been used for cotton fabric, other fibers and polymers. This treatment can reduce costs and environmental impacts. In this study, cotton fabric was treated by corona discharge instrument at 2 - 40 passages and fabrics were washed with laundering method. After four times laundering, shrinkage behavior of corona discharge treated fabric as compared with un-treated fabric decrease. After 10 passages of corona discharge treatment,water, dye absorption and shrinkage are modified but after 14 passages, despite of shrinkage improvement, dyeing properties decrease

A multicenter, prospective, single-arm clinical investigation of a modified staged treatment algorithm using the AeriSeal system:The STAGE trial

Introduction: Treatment with AeriSeal is an alternate treatment option to achieve lung volume reduction in patients with severe COPD and emphysema who are not eligible for valve treatment. This study aimed to assess the safety and mode of action of a modified staged treatment algorithm with a staged treatment with lower dose of AeriSeal. Methods: We performed a prospective, multicenter feasibility study. AeriSeal was administered during two sequential bronchoscopies: 2 subsegments of a lobe treated with two 5 mL doses, followed by two 10 mL doses in a contralateral lobe after 6 weeks. Results: A total of 14 patients (36% male, mean FEV1 28.4% ± 6.7% of predicted) were enrolled. Ten patients completed both treatments, four were treated unilaterally. AeriSeal treatment resulted in significant TLVR (median 220.5 mL) at 3 months follow up. There were no significant changes from baseline at 12 months in lung function, exercise capacity and quality of life. During the 3-month post-treatment period, respiratory SAEs included 5 COPD exacerbations in 4 (28.6%) subjects, post-treatment acute inflammatory response (PAIR) in 2 (14.3%) subjects, and 1 respiratory failure event in 1 (7.1%) subject. Conclusion: The staged and lower dosed administration of AeriSeal does not impact the overall safety profile in terms of reducing the type and frequency of respiratory SAEs previously reported for a single-stage treatment. A larger volume of AeriSeal than used in this study may be necessary to provide meaningful clinical benefits

Protocol of a Randomized Controlled Study of the PneumRx Endobronchial Coil System versus Standard-of-Care Medical Management in the Treatment of Subjects with Severe Emphysema (ELEVATE)

International audienc

Seroepidemiology of human cystic echinococcosis among nomads of lorestan province, Iran

Background: Cystic Echinococcosis (CE) is one of the major zoonotic parasitic diseases caused by the larval stages of parasite Echinococcus granulosus. This is an endemic disease in a number of regions in Iran, such as Lorestan province, and is considered as a health problem. Objectives: Due to the close contact of nomadic people with sheep dog, this research aimed at studying the seroepidemiology of CE among in-migration and out-migration nomads of Lorestan province, Iran. Methods: In this cross-sectional study, among 5920 residents of nomadic areas, 315 people were randomly selected and serum samples were obtained from these individuals. IgG antibodies against CE were evaluated using the Enzyme Linked Immunosorbent Assay (ELISA). Demographic data of all subjects were collected by a questionnaire, and the results were statistically analyzed using SPSS V.20 and STATA12. Results: According to the results, the seroepidemiology of CE among the nomads was 18 (5.7); 13 (72.22) were male and five (27.77) were female yet the difference was not statistically significant. The highest prevalence (7.79) was in the age group of 20 to 30 years old, yet no significant difference was observed between various age groups. In terms of education, more seropositive cases were found amongst subjects with low education, yet no significant difference was observed between educational level and seropositivity of CE. It should also be noted that all the participants were not aware of proper washing of vegetables and fruits. Conclusions: This study was conducted for the first time in Lorestan province, Iran, and showed that the seroprevalence among the nomadic population of Aligoudarz and Sepiddasht was considerable. In order to prevent zoonotic disease, increased awareness of nomads in understanding the transmission ways of disease is suggested through health education programs according to the educational level of the target population, the majority of whom are illiterate. Active surveillance is also recommended in these areas. © 2018, Archives of Clinical Infectious Diseases

Genetic Variants of Cytochrome b-245, Alpha Polypeptide Gene and Premature Acute Myocardial Infarction Risk in An Iranian Population

Background: Oxidative stress induced by superoxide anion plays critical roles in the pathogenesis of coronary artery disease (CAD) and hence acute myocardial infarction (AMI). The major source of superoxide production in vascular smooth muscle and endothelial cells is the NADPH oxidase complex. An essential component of this complex is p22phox, that is encoded by the cytochrome b-245, alpha polypeptide (CYBA) gene. The aim of this study was to investigate the association of CYBA variants (rs1049255 and rs4673) and premature acute myocardial infarction risk in an Iranian population. Methods: The study population consisted of 158 patients under the age of 50 years, with a diagnosis of premature AMI, and 168 age-matched controls with normal coronary angiograms. Genotyping of the polymorphisms was performed by the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Results: There was no association between the genotypes and allele frequencies of rs4673 polymorphism and premature acute myocardial infarction (P>0.05). A significant statistical association was observed between the genotypes distribution of rs1049255 polymorphism and AMI risk (P=0.037). Furthermore, the distribution of AA+AG/GG genotypes was found to be statistically significant between the two groups (P=0.011). Conclusions: Our findings indicated that rs1049255 but not rs4673 polymorphism is associated with premature AMI

Evaluation of Silicon Supplementation for Drought Stress under Water-Deficit Conditions: An Application of Sustainable Agriculture

Drought is a key abiotic stress that confines agriculture development worldwide. Silicon (Si) is commonly considered to be a valuable element for resistance against drought and for sustainable agriculture. To investigate the morpho-physiological and biochemical characteristics of Gerbera jamesonii plants, a pot experiment was conducted under greenhouse conditions and exposed to water stress (60% FC) and well-watered (100% FC) conditions. Foliar application of Si was carried out after ten days (48 days after sowing) of drought treatment and was repeated weekly, while well-water was regarded as control. Water deficiency significantly abridged the morphological attributes, pigments, and stress-related metabolites and negatively affected the photosynthetic apparatus in drought-stressed gerbera plants. However, Si supplementation by 40 mg L-1 produced increased leaf area (31%), stem length (25%), flower diameter (22%), plant fresh biomass (17%), total chlorophyll (48%), and concentration of carotenoids (54%) in water-stressed plants. Similarly, the accretion of a total free amino acid (41%) and the activities of peroxidase, catalase, superoxide dismutase, ascorbate peroxidase, glycinebetaine, total soluble proteins, total free proline, and malondialdehyde were enhanced by 44%, 31%, 53%, 33%, 330%, 61%, 51%, and 66%, respectively, under drought stress in comparison with control conditions. Meanwhile, the photosynthetic rate (89%), the transpiration rate (12%), and stomatal conductance (55%) were significantly enhanced in water-deficit gerbera leaves with Si supplementation. This study proposes that the foliar application of Si is a viable and convenient method of improving the performance of elegant gerbera flower plants in regions of the world that are facing severe water deficiency