Issledovanija prigodnosti toksoplazmoznogo antigena iz tkanevojj kul'tury dlja reakcii svjazyvanija komplmenta

The author compared antigen produced from the tissue culture of infected chicken embryos fibroblasts with antigen produced from mice peritoneal exudate in the complement fixation test. Titration of these antigens showed that exuate antigens are more sensitive (1:1280) than tissue antigen (1:30-1:240). Serum from experimental infected animals and serum samples from people suspected for toxoplasmosis were examined in the complement fixation test parallel with both antigens - tissue and exudate - using in turn: (1) 2 unites of both antigens, (2) 4 unites of both antigens, (3) 4 unites of the exudate antigen, 6 unites of tissue antigen. Only in the last case one received the same results with both antigens

Ocenka teplogo i kholodnogo metoda reakcii svjazyvanija komplementa

287 sera were examined by means of the complement fixation reaction, applying the cold and warm method. 282 were human and 5 rabbit sera. The results of these examinations may be summarized as follows: 1) Positive results were obtained: by the warm method - 5,92%, by the cold method - 14,79%. 2) The titre with the cold method is always higher than the titre obtained with the warm method. 3) The conformity of the results of the complement fixation, Sabin-Feldman's dye test and Frenkel's intradermal test amounts to 61.5% for the warm method and to 66.6% for the cold method. The conformity of the ,results of the complement fixation and Frenkel's intradermal test amounts to 54.8% for the warm and 58.06% for the cold method. The conformity of the results of the complement fixation test and the dye test amounts to 84% for both methods

Sravnenie antigenov prazhskojj i ljublinskojj produkcii vo vnutrikozhnojj probe s toksoplazminom

The authors compared the reaction in 114 women caused by toxoplasmine produced by the Parasitological Department of the "Universita Karlova'' in Prague and that one produced by the Parasitological Department of the Institute of Labour Medicine and Rural Hygiene in Lublin. At an equal dilution of antigens (1 : 1000) the first toxoplasmine induces more frequent and violent skin reactions. Some differences, which could not be nearer explained, were also observed when applying various dilutions. An exact standardization of the antigens aind diagnostic methods for toxoplasmosis should be introduced in order to be able to compare different results

Toksoplazmoz u beremennykh zhenshhin i proba ocenki diagnosticheskikh metodov

The aim of this work was to determine the role of toxoplasmosis in the pathology of pregnancy among women in the Lublin province, and simultaneously the attempt to evaluate the correlation between three diagnostic methods: Frenkel's intradermal test, Sabin-Feldman's dye test and complement fixation reaction. 396 women were examined and 332 simultaneously submitted to serological and allergical investigations. In 64 cases the investigations were only carried out with the complement fixation and intradermal tests. The highest number of positive results was obtained by the intradernal reaction, i. e. 33.8%, the lowest by the complement fixation reaction 8.3%. The dye test gave 10.6%. The conformity of the results for all three tests amounts to 56.7% (225 cases). In the remaining cases (171) the conformity of results between the individual tests was studied, and so: for the complement fixation and the dye tests it amounted to 15.6%, for complement fixation and Frenkel's intradermal tests to 12.5%, for the dye test and Frenkel's intradermal test - 6.7%. The remaining 9,5% fell on the unconformity between the results of the complement fixation reaction and Frenkel's intradermal test. The highest percentage of infections has been observed in the group of premature deliveries; that is 56.6%. To this group belongs also a relatively great number of stillborn children and children with development defects. In the group of women who suffered abortion the percentage of positive results is the lowest – 21.05%. The percentage of positive results is almost identical in the goup of women who were not suspected of toxoplasmosis and in the group where this suspicion existed (32.1%-34,7%). An exact anamnesis concerning the pregnancies of 241 women (multiparae) has shown that the number of abortions in the group of women with positive results is by 50% higher than in the group with negative results. The number of nonviable fetuses is by 2.9 times higher and the number of premature deliveries by 30% higher. The number of infants with development defects is 3 times higher in the group of women who were infected with toxoplasmosis. The number, however, of stillborn children with positive results amounts to 3,8%, while in the group of women with negative results to 5.6%. In cases of habitual abortion, premature delivery or development defects the possibility of toxoplasma infection in the mother should be taken into consideration and laboratorial investigations carried out, such as: complement fixation reaction, Sabin-Feldman's dye test and Ftenkel's intradermal test. A positive results of even one of the serological test in comparison with clinical observations makes an anti-toxoplasmosis treatment unavoidable

Immunobiokhimicheskie issledovanija syvorotok belykh krys ehksperimenyal'no inficirobannykh pri pomoshhi Toxoplasma gondii

White rats (10) were infected with RH Toxoplasma gondii strain administering a Toxoplasma suspension on 7 occasions at 3-day intervals (2 ml of the peritoneal exudate from infected mice, at the dilution of 1:10). The obtained rat sera gave a positive reaction in the complement fixation test with Toxoplasma antigen (1:10-1:160). The electroproteinograms of immune sera (paper electrophoresis, staining according to Grassman-Hannig, photodensitometric measurements) were found to contain albumins and globulins α₁ and α₂, β, γ; as compared with the sera from non-infected rats there were hyperproteinemia and increase in β- and γ- globulins. The inactivation of sera at 56° did not affect the electroproteinogram pattern. Similar results were obtained by electrophoresis of immune sera on gel agar, with the difference that the γ-globulin fraction was extended to γ₁ and γ₂ subfractions. Immunoelectrophoresis (according to Grabar-Williams' and Scheideger's methods) of immune sera, as compared with those of non-infected rat sera, showed more conspicuous precipitation lines in the zones α₂, β and γ as well as a more clear-cut line corresponding to β₂M subfraction. The inactivated sera: gave a smaller number of precipitation lines. Preliminary studies by microdiffusion and agar gel techniques revealed the presence of 5 precipitation lines in the immune sera and only 3 lines in the normal ones. The inactivation of sera diminished the number of precipitation lines to 2 in immune sera, and to 1 in those of non-infected rats. Quantitative determinations of the fractions are under way

Issledovanie sobak v napravlenii toksoplazmoza pri pomoshhi PCK i intradermal'nojj reakcii s toksoplazminom

Shepherd dogs (276), males, at the age of ca. 1 year, from various regions of Poland, were examined. The complement fixation test with the antigen obtained from the exudate of infected mice and intracutaneous test (0.1 ml of toxoplasmine 1:100-1:1000, reading after 24-28 hours, control test with spleen exudate from healthy mice) were performed paralelly. A positive result of both tests was obtained in 10 dogs (4%): in 52 dogs (19%) it was found to occur only with the romplement fixation (titre 1:3-1:8) and in 30 ones (11%) only with the intracutaneous test

Issledovanija chastoty pojavlenija pozityvnykh toksoplazmoznykh reakcijj u detejj s oslableniem umstvennogo razvitija, u glukhikh detejj i u detejj s normal'nym umysvennym razvitiem

The complement fixations (CFT) and intradermal tests (IDT) were carried out in 565 children, including 280 mentally deficient, 179 deaf ones, and a control group of 106 normal subjects. The results are presented in table 1. Similar examinations were made in 104 mothers of mentally deficient. 50 mothers of the deaf and in 49 mothers of normal children. The results are presented in table 2

Experimental treatments with diflubenzuron and deltamethrin of sea bass, Dicentrarchus labrax L., infected with the isopod, Ceratothoa oestroides

Sea bass with approximate average weights of 5 and 20 g were treated against Ceratothoa oestroides infection with: (i) medicated pellets of diflubenzuron PC90 at a dosage of 3 mg kg(-1) body weight (BW) per day for 14 days. Lice were counted at the beginning of treatment and 19 days after treatment. The drug cleared all lice in the treated group; in the control group, infection remained high 30 days after beginning the experiment. It was concluded that medicated pellets containing 3 mg kg(-1) BW diflubenzuron effectively cleared pre-adult and adult stages of the isopod parasite over a 14-day period. No adverse effects were recorded in treated sea bass during the trials and no reinfection occurred 15 days after end of the treatment. (ii) Deltamethrin by means of bath treatments in infected sea bass kept in experimental tanks at 20degreesC. Before treatment, toxicity on healthy fish was preliminarily assessed by testing five fish from each size group at concentrations of 30 10, 5, 3, 1, 0.1, 0.05 and 0.01 mg L-1 for 30 min. The therapeutic concentrations tested were: 10, 5, 3, 0.15, 0.1, and 0.05 mug L-1 and assessed at 1, 24 and 48 h. Best results were achieved with the 10 mug L-1 (0.01 mg L-1) dose, where prevalence was reduced from 100 to 0% over 24 h in both large and small fish. No parasite recovery was observed at 48 h. The dose of 5 mug L-1 reduced prevalence from 100 to 11.7% and to 0% for small and large fish, respectively. Finally, with the 3 mug L-1 dose, prevalence was reduced from 100 to 37.5% (small fish) and to 13.3% (large fish). Lower doses were ineffective on the parasites at either 24 or 48 h