9 research outputs found
Interleukin\u20118 released by cancer\u2011associated fibroblasts attenuates the autophagy and promotes the migration of ovarian cancer cells
The tumor microenvironment composed of a mixture of stromal cells and their secretions has a marked impact on cancer progression. In particular, soluble factors and metabolites contribute to malignancy through the dysregulation of autophagy in cancer cells. The present study investigated the effects of ovarian cancer\u2011associated fibroblasts (OVCAFs) with their secretory substances on the autophagy and migration of ovarian cancer cells. The conditioned\u2011medium (CM) of OVCAFs isolated from fresh human ovarian cancer tissues was analyzed for the levels of 27\ua0common cytokines/chemokines using a cytokine array. Autophagy in cancer cells was assessed by determining the expression of the vacuolar form of LC3 by western blot analysis and immunofluorescence. Cancer cell migration was assessed by Transwell migration assay. Interleukin (IL)\u20118 was found to be the most highly upregulated cytokine among the cytokines/chemokines found in the OVCAF\u2011CM. The role of IL\u20118 in ovarian cancer cell migration and its mechanistic link with autophagy was investigated. Recombinant human IL\u20118 (rhIL\u20118) stimulated the migration of SKOV3 and Kuramochi ovarian cancer cells, and concurrently downregulated basal autophagy, in concentration\u2011dependent manner. Compared to the CM of control counterpart normal fibroblasts isolated from benign ovaries (OVNF\u2011CM), the CM from 3 OVCAF isolates (namely, OVCAF\u20119, \u201120\ua0and\ua0\u201143) exerted effects similar to rhIL\u20118 on both cancer cell lines. The pharmacological induction of autophagy with rapamycin or metformin attenuated the pro\u2011migratory effects of IL\u20118. Neutralizing anti\u2011IL\u20118 antibody counteracted the inhibitory effect of OVCAF\u2011CM on basal autophagy. On the whole, the present study highlights the involvement of IL\u20118 released by CAFs in the ovarian tumor microenvironment in promoting cancer cell migration through the suppression of autophagy
Cancerâassociated fibroblastâderived ilâ6 determines unfavorable prognosis in cholangiocarcinoma by affecting autophagyâassociated chemoresponse
Background: Interleukinâ6 (ILâ6) released by cancerâassociated fibroblasts (CAFs) has been shown to associate with the malignant behavior of cholangiocarcinoma (CCA). Here, we aimed to validate with clinical and molecular data the hypothesis that CAF infiltration and release of ILâ6 predict poor prognosis in CCA patients following dysregulation of autophagy in cancer cells. Methods: Stromal ILâ6 and cancerâcellâassociated autophagy proteins LC3 and p62 were assayed by Tissue MicroArray immunohistochemistry and their expression correlated with overall survival (OS) in a cohort of 70 CCA patients. The 5âFU cytotoxicity and autophagy were determined in CCA cells cultured with CAFâconditioned medium. Results: We show that patients bearing a CCA with low production of stromal ILâ6 and active autophagy flux in the cancer cells have the best prognosis and this correlates with a more effective response to postâoperative chemotherapy. A similar trend was observed in CCA patients from the TCGA database. In vitro genetic manipulation of ILâ6 production by primary CAFs isolated from human CCA showed that ILâ6 impairs the autophagyâassociated apoptotic response to 5âFU in human CCA cells. Stromal ILâ6 inhibition of autophagy in cancer cells was confirmed in an animal model of CCA. Conclusion: Our data support a therapeutic strategy that includes autophagyâenhancing drugs along with adjuvants limiting the stromal inflammation (i.e., the secretion of ILâ6) to improve the survival of CCA patients
Resveratrol contrasts lpaâinduced ovarian cancer cell migration and platinum resistance by rescuing hedgehogâmediated autophagy
Background Ovarian cancer progression and invasiveness are promoted by a range of soluble factors released by cancer cells and stromal cells within the tumor microenvironment. Our previous studies demonstrated that resveratrol (RV), a nutraceutical and caloric restriction mimetic with tumorâsuppressive properties, counteracts cancer cell motility induced by stromal ILâ6 by upregulating autophagy. Lysophosphatidic acid (LPA), a bioactive phospholipid that shows elevated levels in the tumor microenvironment and the ascites of ovarian cancers, stimulates the growth and tissue invasion of cancer cells. Whether LPA elicits these effects by inhibiting autophagy and through which pathway and whether RV can counteract the same remain obscure. Aims To investigate the molecular pathways involved in LPAâinduced ovarian cancer malignancy, particularly focusing on the role of autophagy, and the ability of RV to counteract LPA activity. Results LPA stimulated while RV inhibited ovarian cancer cell migration. Transcriptomic and bioinformatic analyses showed an opposite regulation by LPA and RV of genes linked to epithelial-toâmesenchymal transition (EMT) and autophagy with involvement of the PI3KâAKT, JAKâSTAT and Hedgehog (Hh) pathways. LPA upregulated the Hh and EMT members GLI1, BMIâ1, SNAILâ 1 and TWIST1 and inhibited autophagy, while RV did the opposite. Similar to the inhibitors of the Hh pathway, RV inhibited LPAâinduced cancer cell migration and 3D growth of ovarian cancer cells. BMIâ1 silencing prevented LPAâinduced EMT, restored autophagy and hampered cell migration, resembling the effects of RV. TCGA data analyses indicated that patients with low expression of Hh/EMTârelated genes together with active autophagy flux tended to have a better prognosis and this correlates with a more effective response to platinum therapy. In in vitro 3D spheroids, LPA upregulated BMIâ1, downregulated autophagy and inhibited platinum toxicity while RV and Hh inhibitors restored autophagy and favored BAXâmediated cell death in response to platinum. Conclusions By inhibiting the Hh pathway and restoration of autophagy, RV counteracts LPAâinduced malignancy, supporting its inclusion in the therapy of ovarian cancer for limiting metastasis and chemoresistance