A Study of the Effect of Volume Fraction on Stress Transfer within a Unidirectional Fiber Reinforced Composite Having a Broken Fiber

Stress concentration due to flaws in any material are very dangerous. Its understanding is thus very important before practical application of the material. As Fiber Reinforced Composite (FRC) is gaining wide range of application it is necessary to analyze it for stress concentration as one of the parameter. Literature survey reveals that the failure of FRC due to breakage of fiber is a cumulative process. If a fiber breaks stress concentration develops near the failure which leads to failure of other fibers in its vicinity. This process continues until the whole FRC gets failed. This phenomenon is quantified by a parameter called Stress Transfer Coefficient (STC). To analyze FRC generally it is practiced to analyze Representative Volume Element (RVE) which is a representation of the complete FRC. Another important aspect which is considered while analyzing FRC is the distribution of the fiber. Ideally the distribution of fibers should be uniform but no manufacturing process guarantees uniform distribution. Thus while analyzing FRC it is a good practice to generate RVE with randomly distributed fibers. In this paper an attempt is made to attain the relationship between volume fraction and STC. FRC with unidirectional fiber orientation is considered. RVEs with different volume fraction are analyzed. A new method is implemented to generate RVE with randomly distributed fibers. RVEs are so generated that it contains a broken fiber at its geometrical center and other fibers surrounding it. The RVE is loaded along the fiber direction

Selective promoting activity of phorbol myristate acetate in experimental skin carcinogenesis.

Experiments were undertaken to study the effect of promotion treatment on epidermal tumour induction pattern in precancerous mouse skin. Swiss albino mice were given a single s.c. injection of 0-5 mg 20-methylcholanthrene in the right scapular region. Six weeks later, 1-83 nmol of phorbol myristate acetate (PMA) was applied biweekly on the reactive skin. Histopathology of the induced tumours showed early appearance of squamous cell carcinomas and rhabdomyosarcomas. Fibrosarcoma, the most common tumour type induced on MCA injection alone, was absent. Trichoepithelioma, a benign tumour, was induced in PMA-treated mice. This gives new evidence of the selective action of PMA, enhancing the induction of epithelial and muscle tumours, with concurrent inhibition of fibroblastic tumours

Effect of Volume Fraction and Fiber Distribution on Stress Transfer in a Stochastic Framework of Continuous Fiber Composite: A Micromechanical Study

In fiber Reinforced Composites (FRC) fiber breakage is a common phenomenon resulting in stress concentration. This high stress gets transfer in the vicinity of the breakage which is quantified by Stress Transfer Coefficient (STC). In this paper, an attempt is made to check the effect of fiber volume fraction and the distribution of the fibers on STC and ineffective length. The fiber volume fraction is changed considering three cases: 1) by changing the number of fibers, 2) by changing the dimension of the Represntative Volume Element (RVE) and 3) by changing the fiber radius. Cases with change in dimension of RVE and change in fiber radius, periodic and semi-random arrangents of fibers are considered. From the analysis of 200 RVE's for each volume fraction in random and semi-random arrangements, it is observed that the distribution of STC does not follow any standard distribution, even if the fiber arrangement follows the normal distribution. The fiber cross-sectional dimension plays a critical role in regaining the broken fiber strength. The periodic arrangement of fibers can be said to be beneficial over the random arrangement considering the stress transfer from the broken fiber

Effects of 12-O-tetradecanoylphorbol-13-acetate on the incorporation of labelled precursors into RNA, DNA and protein in epidermis, dermis and subcutis from precancerous mouse skin with reference to enhanced tumorigenesis

The effects of a single application of 1.8 nmol 12-O-tetra-decanoylphorbol-13-acetate (TPA) on precursor incorporation into RNA, DNA and protein in the epidermis, dermis and subcutis from 3-methylcholanthrene (MCA) injected precancerous mouse skin were studied at various time points between 3 and 96 h. In the precancerous tissues, the rates of incorporation of [3H]uridine into RNA did not alter appreciably from those in the control tissues; while the rates of [3H]methylthymidine incorporation into DNA were elevated with peaks appearing between 6 and 12 h, at 24 h and at 72 h in epidermis, dermis and subcutis. The rate of incorporation of [14C] leucine into protein was markedly elevated in all the three tissues which showed 3-4 sharp peaks. The maximum stimulation ranged between 14 and 20 times that of the control. A single application of TPA to the precancerous mouse skin induced early stimulation of precursor incorporation into all the three macromolecules in epidermis, dermis and subcutis. The increased stimulation was maintained for 36- 72 h. The patterns of incorporation of [3H]methylthymidine into DNA gave rise to 2-3 peaks of elevated uptake in each tissue up to 36-48 h. A lowered rate of DNA synthesis between 48 and 60 h was followed by a peak at 72 h. In each group, epidermal mitotic activity correlated well with spurts of precursor incorporation into cellular DNA. The observations indicate that TPA recruits more cells into the DNA synthetic phase and accelerates selective growth of preneoplastic cells during tumor progression

The Role of Thyroid Hormone across Avian Development Spectrum: Investigations on Systemic Development, Metabolism and Ontogeny of Endothermy

Achievement of endothernic capacity is vital for independence from ambient temperature changes, sustained activity, optimal biochemical reactions and optimization of parental care. During early avian development, the core tenets of transition from ectothermy to endothermy are development of metabolic capacity (oxygen consumption, mitochondrial bioenergetics), enhanced cardiovascular function (heart rate and cardiac output), pulmonary ventilation and thermogenic capacity. Thyroid hormones, particularly T3, are key metabolic regulators of basal metabolism, thermogenesis, pulmonary ventilation and mitochondrial respiration. Thyroid hormone fluctuation patterns during both precocial and altricial avian endothermic transition suggest a prominent role in maturation of endothermy, cardiovascular, respiratory and skeletal muscle physiology. This body of work explores effects of T3 manipulations in two avian species: the precocial Pekin duck and the altricial Red-winged Blackbird. Increased plasma T3 during late incubation resulted in increased cardiac mass, elevated resting and intrinsic heart rate, intrinsic mean arterial pressure, increased cholinergic tone and blunted alpha-adrenergic tone in the precocial Pekin duck. In both Pekin duck and Red-winged blackbird, plasma T3 levels correlated with changes in the trajectory of endothermic ontogeny, systemic oxygen consumption, thermogenesis, maturation of pulmonary ventilatory function, altered growth and effects on skeletal and cardiac mitochondrial bioenergetics. These observations support the role of thyroid hormones as metabolic and developmental regulators at the time of attainment of endothermy during the perinatal period in precocial and altricial avian species. Insights into the role of thyroid hormone as a metabolic and development regulator at the time of avian endothermic attainment provide a more thorough understanding of metabolic and physical transitions a hatchling bird must undergo to reach the adult endothermic phenotype. Such insights also deepen understanding of the complex role thyroid hormones play in homeostasis and offer implications about the evolutionary history of endothermic capacity

Post-hatching development of mitochondrial function, organ mass and metabolic rate in two ectotherms, the American alligator (Alligator mississippiensis) and the common snapping turtle (Chelydra serpentina)

The ontogeny of endothermy in birds is associated with disproportionate growth of thermogenic organs and increased mitochondrial oxidative capacity. However, no similar study has been made of the development of these traits in ectotherms. For comparison, we therefore investigated the metabolism, growth and muscle mitochondrial function in hatchlings of a turtle and a crocodilian, two ectotherms that never develop endothermy. Metabolic rate did not increase substantially in either species by 30 days post-hatching. Yolk-free body mass and heart mass did not change through 30 days in alligators and heart mass was a constant proportion of body mass, even after 1 year. Yolk-free body mass and liver mass grew 36% and 27%, respectively, in turtles during the first 30 days post-hatch. The mass-specific oxidative phosphorylation capacity of mitochondria, assessed using permeabilized muscle fibers, increased by a non-significant 47% in alligator thigh and a non-significant 50% in turtle thigh over 30 days, but did not increase in the heart. This developmental trajectory of mitochondrial function is slower and shallower than that previously observed in ducks, which demonstrate a 90% increase in mass-specific oxidative phosphorylation capacity in thigh muscles over just a few days, a 60% increase in mass-specific oxidative phosphorylation capacity of the heart over a few days, and disproportionate growth of the heart and other organs. Our data thus support the hypothesis that these developmental changes in ducks represent mechanistic drivers for attaining endothermy

Lanthanum staining of cell surface and junctional complexes in normal and malignant human oral mucosa

Biopsies from normal and malignant human oral epithelium were studied electron histochemically using lanthanum nitrate for a selective demonstration of junctional complexes at the plasma membrane of adjacent cells. The heavy metal complex was found to settle irregularly, sometimes in intimate proximity to the epithelial cell, in areas corresponding to the glycocalyx, gap junctions, desmosomes and nonspecific contact areas. Tight junctions were rarely seen. The lack of these morphological adhesive areas, the desmosomal detachment seen between cancer cells and the increased lanthanum-positive cell surface may be possible factors responsible for the dissociation of oral malignant cells