Isolation, identification and characterization of new luminous bacteria from Chah Bahar Port, southern marine habitat of Iran

Coastal region of Chah Bahar port, Sea of Oman, was screened for the presence of bioluminescence bacteria for the first time. Water samples were taken from surface and subsurface layers and immediately spread on nutrient seawater complete (SWC) agar. Luminous colonies were observed after an overnight incubation at 25°C. Among twenty luminous isolates, four of them were selected for preliminary bacterial identification based on morphological and physiological characteristics. 16S rRNA genes of selected bacteria were then sequenced in order to be submitted in GenBank database as new strains and performing phylogenetic analysis. Four different submitted bacterial strains are as follow, Vibrio sp. Persian 1, Vibrio sp. Persian 2, Vibrio sp. Persian 3, and Vibrio sp. Persian 4 with accession numbers of KC505639, KC765088, KC765089, and KC896417, respectively. Light emission of isolated luminous bacteria was measured using luminometer. Vibrio sp. Persian 1 was found as the best light emitter with counts per second/OD 600 nm equal to 10 × 10^6 RLU/Sec/OD. Isolated Vibrio species were tested for their ability to form biofilm. Vibrio sp. Persian 3 showed weak ability to produce biofilm while other species were considered as moderate biofilm producers

Isolation and identification of halophilic bacteria from Urmia Lake in Iran

Halophiles are in all three domains of life: Archaea, Bacteria and Eucarya. Halophilic microorganisms in addition to forming a major part of life biodiversity can have many biotechnological applications. The objective of this research is isolation and identification of halophilic bacteria from Urmia Lake in Iran and the study of its bacterial biodiversity. After sampling of brines from Urmia Lake from 10 stations and depth of approximately 30-50 cm, in April 2011 and transfer to the laboratory in the sterile conditions, samples were enriched and cultured on defined media, and incubated. After appearance of colonies, selected strains were studied based on morphology, physiology and biochemical characteristics. For phylogenetic identification, their genomic DNA were extracted and amplified by PCR technique. Therefore their sequences were determined by genetic experiment based on 16S rRNA gene sequence and their similarity were analysed in GenBank of EzTaxon database. Finally the phylogenetic tree was constructed. Studied strains belonged to three genera: Halomonas 50% (including H. andesensis LC6(T) [12.5%], H. gomseomensis M12(T) [12.5%], H. hydrothermalis Slthf2(T) [12.5%], H. boliviensis LC1(T) [6.25%] and H. janggokensis M24(T) [6.25%]), Salinivibrio 25% (including S. costicola subsp. alcaliphilus DSM 16359(T) [18.75%] and S. sharmensis BAG(T) [6.25%]) and Idiomarina 25% (including I. loihiensis L2TR(T) [25%])

The effect of aneurysm geometry on the intra-aneurysmal flow condition

Various anatomical parameters affect on intra-aneurysmal hemodynamics. Nevertheless, how the shapes of real patient aneurysms affect on their intra-aneurysmal hemodynamics remains unanswered. Quantitative computational fluid dynamics simulation was conducted using eight patients’ angiograms of internal carotid artery–ophthalmic artery aneurysms. The mean size of the intracranial aneurysms was 11.5 mm (range 5.8 to 19.9 mm). Intra-aneurysmal blood flow velocity and wall shear stress (WSS) were collected from three measurement planes in each aneurysm dome. The correlation coefficients (r) were obtained between hemodynamic values (flow velocity and WSS) and the following anatomical parameters: averaged dimension of aneurysm dome, the largest aneurysm dome dimension, aspect ratio, and dome–neck ratio. Negative linear correlations were observed between the averaged dimension of aneurysm dome and intra-aneurysmal flow velocity (r = −0.735) and also WSS (r = −0.736). The largest dome diameter showed a negative correlation with intra-aneurysmal flow velocity (r = −0.731) and WSS (r = −0.496). The aspect ratio demonstrated a weak negative correlation with the intra-aneurysmal flow velocity (r = −0.381) and WSS (r = −0.501). A clear negative correlation was seen between the intra-aneurysmal flow velocity and the dome–neck ratio (r = −0.708). A weak negative correlation is observed between the intra-aneurysmal WSS and the dome–neck ratio (r = −0.392). The aneurysm dome size showed a negative linear correlation with intra-aneurysmal flow velocity and WSS. Wide-necked aneurysm geometry was associated with faster intra-aneurysmal flow velocity

Mineral chemistry and thermobarometry of plutonic, metamorphic and anatectic rocks from the Tueyserkan area (Hamedan, Iran)

The study area is a part of the NW to SE striking Sanandaj–Sirjan metamorphic belt in western Iran. The Alvand Pluton, consisting of rocks that range in composition from gabbro to granite, is the major magmatic rock complex of this area. Gabbroic rocks include olivine gabbro, gabbro norite, norite and gabbro. Rocks around the Alvand Pluton were subjected to different P–T conditions due to polymetamorphism. Common metamorphic rocks are meta-pelites, but some meta-psammites, meta-basites and meta-carbonates also occur. Slates, phyllites, schists, migmatites and hornfelses are major rock units of meta-pelites in the metamorphic sequence. Based on mineral chemistry, the highest temperature of crystallisation (1300°C) was determined for the olivine gabbros, and the lowest temperature (950°C) was calculated for the hornblende-bearing gabbros. Clinopyroxene–plagioclase barometry suggests that pressures near 5 to 6 kbars prevailed during the crystallisation of the various mafic rocks. P–T estimates yield maximum temperatures of 700–750°C at 5–6 kbars for the high-grade metamorphic rocks from the metamorphic aureole around the pluton. These results indicate that the heat released from the Alvand Pluton (T = 950–1300°C), which intruded the metamorphic rocks at middle and upper crustal levels, was sufficient to cause partial melting leading to formation of the metatexites, diatexites and restite-rich S-type granites. During this process, part of the deep-seated gabbro-dioritic rocks were transported to higher crustal levels by viscous, enclave- and crystal-rich granitic magmas of the partial melting zone

RANK receptor oligomerisation in the regulation of NFκB signalling

© 2014 The authors.Peer reviewedPublisher PD