Применимость маркеров ISAP, ISSR и SSR в селекционных программах томата

Domesticated crops are characterized by narrow genetic base reflecting one or more bottlenecks during millennia-long selection. As a result, current breeding programs are limited in available germplasm and are forced to deal with incremental improvements of yield, resistance, nutritional value, etc. Since the establishment of modern genetics and biotechnology, several new approaches have emerged to extend the genetic base and germplasm improvement. Among these methods, induced mutagenesis appeared as most useful conventional breeding tool. Although, its successful application currently requires good knowledge of modern molecular tools. In this paper we will make an attempt to overview SSR, ISSR and ISAP techniques as well as to offer examples of their application in tomato breeding programs.За время тысячелетней селекции культурные растения характеризуются зауженностью генетической основы, отражающейся в одном и нескольких эффектах "бутылочного горлышка". В результате направленной селекционной работы потенциал имеющихся генетических ресурсов становится ограниченным, и требуется дальнейшая работа по поиску генресурсов для улучшения урожайности, устойчивости, пищевой ценности и т.д. С открытием современных методов генетики и биотехнологии некоторые достижения уже используются для улучшения потенциального использование генетических ресурсов. Среди этих методов индуцированный мутагенез можно рассматривать как наиболее полезный для традиционной селекции, хотя его широкое использование требует хороших знаний в области современных молекулярных технологий. В данной публикации мы сделали обзор по использованию SSR, ISSR и ISAP методов и привели примеры их конкретного применения в селекции томата

Applicability of ISAP, ISSR and SSR markers in tomato breeding programs

Domesticated crops are characterized by narrow genetic base reflecting one or more bottlenecks during millennia-long selection. As a result, current breeding programs are limited in available germplasm and are forced to deal with incremental improvements of yield, resistance, nutritional value, etc. Since the establishment of modern genetics and biotechnology, several new approaches have emerged to extend the genetic base and germplasm improvement. Among these methods, induced mutagenesis appeared as most useful conventional breeding tool. Although, its successful application currently requires good knowledge of modern molecular tools. In this paper we will make an attempt to overview SSR, ISSR and ISAP techniques as well as to offer examples of their application in tomato breeding programs

Effects of Leptin on Intestinal Ischemia–Reperfusion Injury

Many clinical conditions such as shock, sepsis, mesenteric thrombosis, necrotizing enterocolitis, and bowel transplantation can cause intestinal ischemia–reperfusion (IR) injury. This study was designed to determine the effects of leptin on intestinal IR injury. Thirty rats were divided into three groups, each containing ten rats: group A (IR group), group B (treatment group), and group C (sham group). After 1 h of intestinal ischemia, the clamp was removed in order to perform reperfusion. In group B, 100 mg/kg leptin was administered subcutaneously 30 min before reperfusion. In groups A and C, 0.1 ml physiologic saline was injected. In group A, serum and tissue nitric oxide (NO) levels were significantly decreased, and malondialdehyde levels were significantly increased compared to sham group (p < 0.05). Histopathologic injury was significantly lower in sham group compared to group A. In group B, serum and tissue malondialdehyde levels were significantly decreased (p < 0.05), but serum and tissue NO levels were significantly increased compared to group A (p < 0.05). Histopathologic injury was significantly lower in group B compared to group A (p < 0.05). The results of the present study demonstrated that leptin decreases intestinal IR injury by increasing NO production, rearranging mucosal blood flow, and inhibiting polymorphonuclear leukocyte infiltration