Leadership: Untapping the secret to regional wellbeing, belonging and resilience

Work holds a key place within modern urban life, and this is no less within regions. In this chapter, we explore leadership styles and discuss the importance that psychological contracts have in the employment relationship and how they influence employee wellbeing. Any breaches of the psychological contract may prove detrimental to the wellbeing of the individual, the organisation and the wider community. To highlight this, we provide a detailed example of a breach of contract in a regional area where workers, their families and the community reported feeling betrayed after years of work, dedication and loyalty to organisation. In this context, we also explore leadership in a range of regional customer service organisations. Findings from this empirical evidence suggest that when workplace leadership includes components of the psychological contract, wellbeing, resilience and belonging outcomes emerge. Such positive outcomes have important implications for promoting healthy regions and their communities

Identification of target genes in breast cancer cells directly regulated by the SRC-3/AIB1 coactivator

Steroid receptor coactivator-3 (SRC-3/AIB1) is a coactivator for nuclear receptors and other transcription factors and an oncogene that contributes to growth regulation and development of mammary and other tumor types. Because of its biological functions, it is important to identify genes regulated by SRC-3. However, because coactivators do not bind DNA directly, extensive work is required to determine whether genes identified by RNA profiling approaches are direct or indirect targets. Here, we report the use of chromatin immunoprecipitation (ChIP)-based assays that involve genomic mapping and computational analyses of immunoprecipitated DNA to identify SRC-3-binding target genes in estradiol (E2)-treated MCF-7 breast cancer cells. We identified 18 SRC-3 genomic binding sites and demonstrated estrogen receptor-α (ERα) binding to all of them. Both E2-dependent and -independent SRC-3/ERα-binding sites were identified. RNA polymerase II ChIP assays were used to determine the correlation between SRC-3 and ERα binding and recruitment of the transcriptional machinery. These assays, in conjunction with analyses of RNA obtained from E2-treated cells, lead to the identification of SRC-3/ERα-associated genes. The ability of SRC family coactivators to regulate the expression of one of these genes, PARD6B/Par6, was confirmed by using cells individually depleted of SRC-1, SRC-2, or SRC-3 by small interfering RNA. The method described herein can be used to identify genes regulated by non-DNA-binding factors, such as other coactivators or corepressors, as well as DNA-binding transcription factors, and provides information on their binding location that can accelerate further gene characterization