Direct Observation of Dimerization between Different CREB1 Isoforms in a Living Cell

Cyclic AMP-responsive element binding protein 1 (CREB1) plays multiple functions as a transcription factor in gene regulation. CREB1 proteins are also known to be expressed in several spliced isoforms that act as transcriptional activators or repressors. The activator isoforms, possessing the functional domains for kinase induction and for interaction with other transcriptional regulators, act as transcriptional activators. On the other hand, some isoforms, lacking those functional domains, are reported to be repressors that make heterodimers with activator isoforms. The complex and ingenious function for CREB1 arises in part from the variation in their spliced isoforms, which allows them to interact with each other. To date, however, the dimerization between the activator and repressor isoforms has not yet been proved directly in living cells. In this study, we applied fluorescence cross-correlation spectroscopy (FCCS) to demonstrate direct observation of dimerization between CREB1 activator and repressor. The FCCS is a well established spectroscopic method to determine the interaction between the different fluorescent molecules in the aqueous condition. Using differently labeled CREB1 isoforms, we successfully observed the interaction of CREB1 activator and repressor via dimerization in the nuclei of cultured cells. As a result, we confirmed the formation of heterodimer between CREB1 activator and repressor isoforms in living cells

Search for Light Gluinos via the Spontaneous Appearance of pi+pi- Pairs with an 800 GeV/c Proton Beam at Fermilab

We searched for the appearance of pi+pi- pairs with invariant mass greater than 648 MeV in a neutral beam. Such an observation could signify the decay of a long-lived light neutral particle. We find no evidence for this decay. Our null result severely constrains the existence of an R0 hadron, which is the lightest bound state of a gluon and a light gluino, and thereby also the possibility of a light gluino. Depending on the photino mass, we exclude the R0 in the mass and lifetime ranges of 1.2 -- 4.6 GeV and 2E-10 -- 7E-4 seconds, respectively. (To Appear in Phys. Rev. Lett.)Comment: Documentstyle aps,epsfig,prl (revtex), 6 pages, 7 figure

A Measurement of the KL Charge Asymmetry

We present a measurement of the charge asymmetry $\delta_L$ in the mode $K_L \to \pi^{\pm}e^{\mp}\nu$ based on 298 million analyzed decays. We measure a value of $\delta_L = (3322 \pm 58(stat) \pm 47(sys))\cdot 10^{-6}$, in good agreement with previous measurements and 2.4 times more precise than the current best published result. The result is used to place more stringent limits on CPT and $\Delta S = \Delta Q$ violation in the neutral kaon system.Comment: Submitted to Physical Review Letters, Dec 31, 2001. 4 pages, 4 figure

Search for the Decay K_L -> pi^0 nu nubar using pi^0 -> e^+ e^- gamma

We report on a search for the decay K_L -> pi^0 nu nubar, carried out as a part of E799-II, a rare K_L decay experiment at Fermilab. Within the Standard Model, the K_L -> pi^0 nu nubar decay is dominated by direct CP violating processes, and thus an observation of the decay implies confirmation of direct CP violation. Due to theoretically clean calculations, a measurement of B(K_L -> pi^0 nu nubar) is one of the best ways to determine the CKM parameter eta. No events were observed, and we set an upper limit B(K_L -> pi^0 nu nubar) < 5.9 times 10^-7 at the 90% confidence level.Comment: 5 pages, 4 figure

First Observation of the decay KL -> pi0 e e gamma

We report on the first observation of the decay KL -> pi0 ee gamma by the KTeV E799 experiment at Fermilab. Based upon a sample of 48 events with an estimated background of 3.6 +/- 1.1 events, we measure the KL -> pi0 ee gamma branching ratio to be (2.34 +/- 0.35 +/- 0.13)x10^{-8}. Our data agree with recent O(p^6) calculations in chiral perturbation theory that include contributions from vector meson exchange through the parameter a_V. A fit was made to the KL -> pi0 ee gamma data for a_V with the result -0.67 +/- 0.21 +/- 0.12, which is consistent with previous results from KTeV.Comment: Submitted to Physical Review Letters, 5 pages, 5 figure

Search for the Decay KL→π0μ+μ−K_L \to \pi^0 \mu^+ \mu^-

We report on a search for the decay \klpimumu carried out as a part of the KTeV experiment at Fermilab. This decay is expected to have a significant $CP$ violating contribution and a direct measurement will either support the CKM mechanism for CP violation or point to new physics. Two events were observed in the 1997 data with an expected background of $0.87 \pm 0.15$ events, and we set an upper limit \BR{\klpimumu} $<3.8 \times 10^{-10}$ at the 90% confidence level.Comment: See also "Observation of the Decay $K_L\to \mu^+\mu^- \gamma \gamma$", also by the KTeV collaboratio

Search for the Decay Kl -> pi0 e+ e-

We report on a search for the decay Kl -> pi0 e+ e- carried out by the KTeV/E799 experiment at Fermilab. This decay is expected to have a significant CP violating contribution and the measurement of its branching ratio could support the CKM mechanism for CP violation or could point to new physics. Two events were observed in the 1997 data with an expected background of 1.06 +-0.41 events, and we set an upper limit Br(Kl -> pi0 e+ e-) < 5.1 x 10^-10 at the 90% confidence level.Comment: 13 pages, 2 figure

Observation of the Decay KL→μ+μ−γγK_L\to \mu^+\mu^- \gamma \gamma

We have observed the decay $K_L\to \mu^+\mu^- \gamma \gamma$ at the KTeV experiment at Fermilab. This decay presents a formidable background to the search for new physics in $K_L\to\pi^0\mu^+\mu^-$. The 1997 data yielded a sample of 4 signal events, with an expected background of 0.155 $\pm$ 0.081 events. The branching ratio is ${\mathcal B}(K_L\to \mu^+\mu^- \gamma \gamma$) $= (10.4^{+7.5}_{-5.9} {\rm (stat)} \pm 0.7 {\rm (sys)})\times 10^{-9}$ with $m_{\gamma\gamma} \geq 1 {\rm MeV/c}^2$, consistent with a QED calculation which predicts $(9.1\pm 0.8)\times 10^{-9}$.Comment: See also the paper "Search for the Decay $K_L \to \pi^0 \mu^+ \mu^-$", also by the KTeV collaboratio

Measurement of the branching ratio of pi^0 -> e^+e^- using K_L -> 3 pi^0 decays in flight

The branching ratio of the rare decay pi^0 -> e^+e^- has been measured in E799-II, a rare kaon decay experiment using the KTeV detector at Fermilab. The pi^0's were produced in fully-reconstructed K_L -> 3 pi^0 decays in flight. We observed 275 candidate pi^0 -> e^+e^- events, with an expected background of 21.4 +- 6.2 events which includes the contribution from Dalitz decays. We measured BR(pi^0 -> e^+e^-, x>0.95) = (6.09 +- 0.40 +- 0.24) times 10^{-8}, where the first error is statistical and the second systematic. This result is the first significant observation of the excess rate for this decay above the unitarity lower bound.Comment: New version shortened to PRL length limit. 5 pages, 4 figures. Published in Phys. Rev. Let

Measurement of the Decay KL -> Pi0 Gamma Gamma

We report on a new measurement of the decay KL -> pi0 gamma gamma by the KTeV experiment at Fermilab. We determine the KL -> pi0 gamma gamma branching ratio to be (1.68 +/- 0.07 +/- 0.08)x10**-6. Our data shows the first evidence for a low-mass gamma gamma signal as predicted by recent O(p**6) chiral perturbation calculations that include vector meson exchange contributions. From our data, we extract a value for the effective vector coupling aV = -0.72 +/- 0.05 +/- 0.06.Comment: 5 pages, 4 figure