Estrogen Deficiency and Diet Differentially Regulate Goblet Cell Count and Inflammation in Gut

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fat - 1 overexpression mitigates the weight gain and intestinal inflammation in ovariectomized mice on high - fat diets

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Overground walking speed changes when subjected to body weight support conditions for nonimpaired and post stroke individuals

<p>Abstract</p> <p>Background</p> <p>Previous research has shown that body weight support (BWS) has the potential to improve gait speed for individuals post-stroke. However, body weight support also reduces the optimal walking speed at which energy use is minimized over the gait cycle indicating that BWS should reduce walking speed capability.</p> <p>Methods</p> <p>Nonimpaired subjects and subjects post-stroke walked at a self-selected speed over a 15 m walkway. Body weight support (BWS) was provided to subjects at 0%, 10%, 20%, 30%, and 40% of the subject's weight while they walked overground using a robotic body weight support system. Gait speed, cadence, and average step length were calculated for each subject using recorded data on their time to walk 10 m and the number of steps taken.</p> <p>Results</p> <p>When subjected to greater levels of BWS, self-selected walking speed decreased for the nonimpaired subjects. However, subjects post-stroke showed an average increase of 17% in self-selected walking speed when subjected to some level of BWS compared to the 0% BWS condition. Most subjects showed this increase at the 10% BWS level. Gait speed increases corresponded to an increase in step length, but not cadence.</p> <p>Conclusions</p> <p>The BWS training environment results in decreased self-selected walking speed in nonimpaired individuals, however self-selected overground walking speed is facilitated when provided with a small percentage of body weight support for people post-stroke.</p

The Cytosolic Tail of the Golgi Apyrase Ynd1 Mediates E4orf4-Induced Toxicity in Saccharomyces cerevisiae

The adenovirus E4 open reading frame 4 (E4orf4) protein contributes to regulation of the progression of virus infection. When expressed individually, E4orf4 was shown to induce non-classical transformed cell-specific apoptosis in mammalian cells. At least some of the mechanisms underlying E4orf4-induced toxicity are conserved from yeast to mammals, including the requirement for an interaction of E4orf4 with protein phosphatase 2A (PP2A). A genetic screen in yeast revealed that the Golgi apyrase Ynd1 associates with E4orf4 and contributes to E4orf4-induced toxicity, independently of Ynd1 apyrase activity. Ynd1 and PP2A were shown to contribute additively to E4orf4-induced toxicity in yeast, and to interact genetically and physically. A mammalian orthologue of Ynd1 was shown to bind E4orf4 in mammalian cells, confirming the evolutionary conservation of this interaction. Here, we use mutation analysis to identify the cytosolic tail of Ynd1 as the protein domain required for mediation of the E4orf4 toxic signal and for the interaction with E4orf4. We also show that E4orf4 associates with cellular membranes in yeast and is localized at their cytoplasmic face. However, E4orf4 is membrane-associated even in the absence of Ynd1, suggesting that additional membrane proteins may mediate E4orf4 localization. Based on our results and on a previous report describing a collection of Ynd1 protein partners, we propose that the Ynd1 cytoplasmic tail acts as a scaffold, interacting with a multi-protein complex, whose targeting by E4orf4 leads to cell death

Level of Caregiver Burden in Jamaican Stroke Caregivers and Relationship between Selected Socio-Demographic Variables

OBJECTIVE: This study sought to determine the level of caregiver burden present in Jamaican stroke caregivers and to investigate the relationship between caregiver burden and selected sociodemographic variables. METHOD: The Bakas Caregiving Outcomes Scale and a demographic sheet were administered to the caregivers. The stroke survivors completed the Barthel Index and the Geriatric Depression Scale. Interview administration was used for all tools. RESULTS: A total of 48 caregivers with their stroke survivors participated in this study. Preliminary findings showed that caregivers of stroke survivors in Jamaica are experiencing some level of burden with scores ranging from 21 to 62 (mean = 43.64 ± 8.73). Lower burden scores indicate greater burden. Caregivers of female stroke survivors had significantly greater levels of burden (43.5 ± 8.87) than those caring for male survivors [44.4 ± 8.3] (p = 0.03). There were no significant correlations between caregiver burden and age of the stroke survivor; however, a low correlation was found between depression and caregiver burden (r = −0.33, p = 0.02). Female survivors were found to have significantly higher levels of depression than males (16.72 ± 6.21 vs 11.34 ± 7.31, p < 0.01) and caregivers of survivors who had higher depression scores experienced more burden. CONCLUSION: Preliminary findings point to depressive symptoms and functional status in the stroke survivor as being a significant contributor to caregiver burden; however, further data collection and analysis need to be completed before more definitive conclusions can be made

Plyometric Training Improves Power and Agility in Jamaica’s National Netball Team

Objective: Plyometric training is widely used in conditioning, power training and in prevention and rehabilitation of injuries in some sports. This study sought to investigate the effect of a three-week plyometric training programme on jump performance and agility in Jamaican national netball players. Method: Eighteen national netballers participated in a Plyometric training programme. Subjects were evaluated using the Vertical Jump Test, the Broad Jump Test and the Illinois Agility Test prior to the start of the programme and at week 3. Data Analysis: The data were analysed using SPSS version 12 for Windows. A One-Sample Kolmogorov-Smirnov Test showed normal distribution of data and a paired samples t-test was used to determine whether the mean change in jump performance and agility was significant. Results: All eighteen subjects completed the three weeks of training. Three subjects were shown to be outliers at week 3 and this resulted in data from 15 subjects being analysed. At the end of the three weeks, there were significant improvements in Vertical Jump Scores (p = 0.023), Broad Jump Scores (p = 0.002) and Agility scores (p = 0.045). Conclusion: Three weeks of Plyometric training can lead to significant improvements in jump performance and agility and should be integrated into the national training programme at intervals yet to be determined. Keywords: Illinois Agility Test, netballers, plyometric training "El Entrenamiento Pliométrico Mejora la Fuerza y Agilidad del Equipo Nacional de Netball de Jamaica" RESUMEN Objetivo: El entrenamiento pliométrico se usa ampliamente para el acondicionamiento, y entrenamiento de fuerza, así como para la prevención y rehabilitación de lesiones en algunos deportes. Este estudio buscó investigar el efecto de un programa de entrenamiento pliométrico de tres semanas sobre el rendimiento en salto alto y la agilidad de las jugadoras del Equipo Nacional de Netball de Jamaica. Método: Dieciocho jugadoras nacionales de netball participaron en un programa de entrenamiento pliométrico. Las sujetos fueron evaluadas usando la prueba de salto vertical, la prueba de salto largo y la prueba de agilidad de Illinois, antes del comienzo del programa y a la semana 3. Análisis de datos: Los datos fueron analizados usando la versión 12 de SPSS para Windows. La Prueba de Kolmogorov-Smirnov para una muestra mostró una distribución normal de los datos y una prueba t de muestras pareadas fue usada para determinar si el cambio promedio en el rendimiento del salto y la agilidad fue significativo. Resultados: Las dieciocho sujetos completaron las tres semanas de entrenamiento. Tres sujetos mostraron tener valores atípicos en la semana 3, y esto trajo por resultado que se analizaran datos de 15 sujetos. Al final de las tres semanas, se produjeron mejoras significativas en las puntuaciones del salto vertical (p = 0.023), las puntuaciones del salto largo (p = 0.002) y las puntuaciones de la agilidad (p = 0.045). Conclusión: Tres semanas de entrenamiento pliométrico pueden llevar a mejoras significativas en el rendimiento y agilidad, y deben integrarse en el programa de entrenamiento nacional a intervalos aún por determinar. Palabras claves: Prueba de agilidad de Illinois, jugadoras de netball, entrenamiento pliométric