Interview with Norm Reef by Mike Hastings

Biographical NoteNorman S. “Norm” Reef was born on August 16, 1933, in Portland, Maine, where he grew up with his parents, Samuel Reef and Dora Reef, and seven siblings. His father emigrated from Lithuania at age fourteen and worked as a cobbler. Norm grew up in the Jewish community of Portland, and the family struggled to make ends meet during the Depression and World War II. He attended Boston University and after two years joined the Army; the Korean War ended just as he completed training. He returned to study public relations at Boston University and then attended law school to help with his brothers’ insurance finance business. After being married, he set up a law practice in Portland. He became part of the fund-raising group for the Maine Democratic Party and encouraged Governor Brennan to appoint Mitchell to the Senate. He has also done legal work for Mitchell and is a personal friend. His daughter, Grace Reef, worked as a legislative assistant in Mitchell’s U.S. Senate office. He retired in 1989 from full-time practice of law. At the time of this interview, he was working on bringing about the production of a patent he holds for an industrial waste incinerator that generates power, associated with the firm Maine Microfurnace. SummaryInterview includes discussion of: family and educational background; the Jewish community in Portland; growing up in a large family during WWII; the Army and being a dental technician in Texas; Boston University and being president of a fraternity; working with his brothers and going to law school; joining the finance committee for the Maine Democratic Party; Muskie’s swearing in as secretary of state 1980; meeting President Clinton; why Mitchell lost the 1974 gubernatorial race; a meeting in Augusta and Brennan’s decision to appoint Mitchell to the Senate; Mitchell at hockey games and his involvement with the Red Sox; Mitchell’s relationship to Muskie; Mitchell as U.S. attorney; humorous anecdotes about Grace’s internship in Mitchell’s office; Reef’s retirement and return to work; the Microfurnace patent; and Mitchell’s exceptional character

Global coastal wetland change under sea-level rise and related stresses: The DIVA Wetland Change Model

The Dynamic Interactive Vulnerability Assessment Wetland Change Model (DIVA_WCM) comprises a dataset of contemporary global coastal wetland stocks (estimated at 756 × 10^3 km^2 (in 2011)), mapped to a one-dimensional global database, and a model of the macro-scale controls on wetland response to sea-level rise. Three key drivers of wetland response to sea-level rise are considered: 1) rate of sea-level rise relative to tidal range; 2) lateral accommodation space; and 3) sediment supply. The model is tuned by expert knowledge, parameterised with quantitative data where possible, and validated against mapping associated with two large-scale mangrove and saltmarsh vulnerability studies. It is applied across 12,148 coastal segments (mean length 85 km) to the year 2100. The model provides better-informed macro-scale projections of likely patterns of future coastal wetland losses across a range of sea-level rise scenarios and varying assumptions about the construction of coastal dikes to prevent sea flooding (as dikes limit lateral accommodation space and cause coastal squeeze). With 50 cm of sea-level rise by 2100, the model predicts a loss of 46–59% of global coastal wetland stocks. A global coastal wetland loss of 78% is estimated under high sea-level rise (110 cm by 2100) accompanied by maximum dike construction. The primary driver for high vulnerability of coastal wetlands to sea-level rise is coastal squeeze, a consequence of long-term coastal protection strategies. Under low sea-level rise (29 cm by 2100) losses do not exceed ca. 50% of the total stock, even for the same adverse dike construction assumptions. The model results confirm that the widespread paradigm that wetlands subject to a micro-tidal regime are likely to be more vulnerable to loss than macro-tidal environments. Countering these potential losses will require both climate mitigation (a global response) to minimise sea-level rise and maximisation of accommodation space and sediment supply (a regional response) on low-lying coasts.The authors gratefully acknowledge funding from the European Union under contract number EVK2-2000-22024. They thank all their partners in the DINAS-COAST project Dynamic and Interactive Assessment of National, Regional and Global Vulnerability of Coastal Zones to Climate Change and Sea-level rise. We are grateful to staff at UNEP-WCMC for generous access to evolving databases on global coastal wetland extent: Jon Hutton, Hannah Thomas, Jan-Willem van Bochove, Simon Blyth and Chris McOwen. Current wetland databases held at WCMC build upon the pioneering efforts of Mark Spalding and Carmen Lacambra.This is the author accepted manuscript. The final version is available from Elsevier via http://dx.doi.org/10.1016/j.gloplacha.2015.12.01

Transabdominal ultrasonographic evaluation of fetal well-being in the late-term mare and cow

In the equine practice, attempts have been made to examine the fetus in the second and third trimester of pregnancy but all of the available methods have limitations. Until now, transabdominal ultrasonography has been regarded as the most informative examination. This method allows us to measure fetal heart rate, fetal activity as well as the quality and quantity of the fetal fluids. A modified biophysical profile for horses was used by several researchers in the USA from the 1990s as a gold standard. However, it is not sensitive enough and, in the authors’ experience, professionals can face difficulties during its application (e.g. for measuring aortic diameter and fetal breathing movements). In cows, this method was first used for this purpose by a Canadian research group in 2007. They reported that transabdominal ultrasound was promising but showed low sensitivity in this species. The present studies show that birth weight cannot be predicted from fetal aortic diameter measurement in cows as suggested by other researchers. Transabdominal ultrasound needs special equipment (2–3.5 MHz convex transducer) and basic ultrasonographic knowledge; however, we suggest that in most cases it can be performed with the dam placed in a stock and without shaving the examination area. The method provides useful information within 30–40 minutes, enabling the examiner to determine whether or not the fetus is alive and to recognise placentitis or twins. This technique also allows measuring the combined thickness of the uteroplacental unit, and the authors’ ongoing study showed higher normal values in Lipizzaner mares compared to values in other breeds. In conclusion, with the help of advanced techniques, simple and low-cost methods should be developed for the evaluation of the pregnant dam and its fetus to assess fetal viability in the veterinary practice

Fetal heart rate and fetal heart rate variability in Lipizzaner broodmares

Monitoring fetal heart rate (FHR) and fetal heart rate variability (FHRV) helps to understand and evaluate normal and pathological conditions in the foal. The aim of this study was to establish normal heart rate reference values for the ongoing equine pregnancy and to perform a heart rate variability (HRV) time-domain analysis in Lipizzaner mares. Seventeen middle- and late-term (days 121–333) pregnant Lipizzaner mares were examined using fetomaternal electrocardiography (ECG). The mean FHR (P = 0.004) and the standard deviation of FHR (P = 0.012) significantly decreased during the pregnancy. FHR ± SD values decreased from 115 ± 35 to 79 ± 9 bpm between months 5 and 11. Our data showed that HRV in the foal decreased as the pregnancy progressed, which is in contrast with the findings of earlier equine studies. The standard deviation of normal-normal intervals (SDNN) was higher (70 ± 25 to 166 ± 108 msec) than described previously. The root mean square of successive differences (RMSSD) decreased from 105 ± 69 to 77 ± 37 msec between the 5th and 11th month of gestation. Using telemetric ECG equipment, we could detect equine fetal heartbeat on day 121 for the first time. In addition, the large differences observed in the HR values of four mare-fetus pairs in four consecutive months support the assumption that there might be ‘high-HR’ and ‘low-HR’ fetuses in horses. It can be concluded that the analysis of FHR and FHRV is a promising tool for the assessment of fetal well-being but the applicability of these parameters in the clinical setting and in studs requires further investigation

Photoreactivation is the main repair pathway for UV-induced DNA damage in coral planulae

The larvae of most coral species spend some time in the plankton, floating just below the surface and hence exposed to high levels of ultraviolet radiation (UVR). The high levels of UVR are potentially stressful and damaging to DNA and other cellular components, such as proteins, reducing survivorship. Consequently, mechanisms to either shade (prevent) or repair damage potentially play an important role. In this study, the role of photoreactivation in the survival of coral planulae was examined. Photoreactivation is a light-stimulated response to UV-damaged DNA in which photolyase proteins repair damaged DNA. Photoreactivation rates, as well as the localization of photolyase, were explored in planulae under conditions where photoreactivation was or was not inhibited. The results indicate that photoreactivation is the main DNA repair pathway in coral planulae, repairing UV-induced DNA damage swiftly (K=1.75 h–1 and a half-life of repair of 23 min), with no evidence of any light-independent DNA repair mechanisms, such as nucleotide excision repair (NER), at work. Photolyase mRNA was localized to both the ectoderm and endoderm of the larvae. The amount of cell death in the coral planulae increased significantly when photoreactivation was inhibited, by blocking photoreactivating light. We found that photoreactivation, along with additional UV shielding in the form of five mycosporine-like amino acids, are sufficient for survival in surface tropical waters and that planulae do not accumulate DNA damage despite being exposed to high UVR

Assessment of Equine Autoimmune Thrombocytopenia (EAT) by flow cytometry

RATIONALE: Thrombocytopenia is a platelet associated process that occurs in human and animals as result of i) decreased production; ii) increased utilization; iii) increased destruction coupled to the presence of antibodies, within a process know as immune-mediated thrombocytopenia (IMT); or iv) platelet sequestration. Thus, the differentiation of the origin of IMT and the development of reliable diagnostic approaches and methodologies are important in the clarification of IMT pathogenesis. Therefore, there is a growing need in the field for easy to perform assays for assessing platelet morphological characteristics paired with detection of platelet-bound IgG. OBJECTIVES: This study is aimed to develop and characterize a single color flow cytometric assay for detection of platelet-bound IgG in horses, in combination with flow cytometric assessment of platelet morphological characteristics. FINDINGS: The FSC and SSC evaluation of the platelets obtained from the thrombocytopenic animals shows several distinctive features in comparison to the flow cytometric profile of platelets from healthy animals. The thrombocytopenic animals displayed i) increased number of platelets with high FSC and high SSC, ii) a significant number of those gigantic platelets had strong fluorescent signal (IgG bound), iii) very small platelets or platelet derived microparticles were found significantly enhanced in one of the thrombocytopenic horses, iv) significant numbers of these microplatelet/microparticles/platelet-fragments still carry very high fluorescence. CONCLUSIONS: This study describes the development and characterization of an easy to perform, inexpensive, and noninvasive single color flow cytometric assay for detection of platelet-bound IgG, in combination with flow cytometric assessment of platelet morphological characteristics in horses

Atrial fibrillatory rate as predictor of recurrence of atrial fibrillation in horses treated medically or with electrical cardioversion

Background The recurrence rate of atrial fibrillation (AF) in horses after cardioversion to sinus rhythm (SR) is relatively high. Atrial fibrillatory rate (AFR) derived from surface ECG is considered a biomarker for electrical remodelling and could potentially be used for the prediction of successful AF cardioversion and AF recurrence. Objectives Evaluate if AFR was associated with successful treatment and could predict AF recurrence in horses. Study design Retrospective multicentre study. Methods Electrocardiograms (ECG) from horses with persistent AF admitted for cardioversion with either medical treatment (quinidine) or transvenous electrical cardioversion (TVEC) were included. Bipolar surface ECG recordings were analysed by spatiotemporal cancellation of QRST complexes and calculation of AFR from the remaining atrial signal. Kaplan-Meier survival curve and Cox regression analyses were performed to assess the relationship between AFR and the risk of AF recurrence. Results Of the 195 horses included, 74 received quinidine treatment and 121 were treated with TVEC. Ten horses did not cardiovert to SR after quinidine treatment and AFR was higher in these, compared with the horses that successfully cardioverted to SR (median [interquartile range]), (383 [367-422] vs 351 [332-389] fibrillations per minute (fpm), P < .01). Within the first 180 days following AF cardioversion, 12% of the quinidine and 34% of TVEC horses had AF recurrence. For the horses successfully cardioverted with TVEC, AFR above 380 fpm was significantly associated with AF recurrence (hazard ratio = 2.4, 95% confidence interval 1.2-4.8, P = .01). Main limitations The treatment groups were different and not randomly allocated, therefore the two treatments cannot be compared. Medical records and the follow-up strategy varied between the centres. Conclusions High AFR is associated with failure of quinidine cardioversion and AF recurrence after successful TVEC. As a noninvasive marker that can be retrieved from surface ECG, AFR can be clinically useful in predicting the probability of responding to quinidine treatment as well as maintaining SR after electrical cardioversion

Global Distribution of Rubella Virus Genotypes

Phylogenetic analysis of a collection of 103 E1 gene sequences from rubella viruses isolated from 17 countries from 1961 to 2000 confirmed the existence of at least two genotypes. Rubella genotype I (RGI) isolates, predominant in Europe, Japan, and the Western Hemisphere, segregated into discrete subgenotypes; intercontinental subgenotypes present in the 1960s and 1970s were replaced by geographically restricted subgenotypes after ~1980. Recently, active subgenotypes include one in the United States and Latin America, one in China, and a third that apparently originated in Asia and spread to Europe and North America, starting in 1997, indicating the recent emergence of an intercontinental subgenotype. A virus that potentially arose as a recombinant between two RGI subgenotypes was discovered. Rubella genotype II (RGII) showed greater genetic diversity than did RGI and may actually consist of multiple genotypes. RGII viruses were limited to Asia and Europe; RGI viruses were also present in most of the countries where RGII viruses were isolated

Author Correction: Future response of global coastal wetlands to sea-level rise.

Change history: In Fig. 2b of this Letter, 'Relative wetland change (km2)' should have read 'Relative wetland change (%)' and equations (2) and (3) have been changed from 'RSLRcrit = (m × TRe) × Sed + i' and 'Sedcrit = (RSLR - i)/(m × TRe)', respectively. The definition of the variables in equation (2) has been updated. These errors have been corrected online