" Sensitivity analysis of damaged monumental structures: the example of S. Maria del Suffragio in L'Aquila "

In Italy, which accounts for an impressive number of architectural heritage sites, a large part of the territory is subject to seismic risk. Nonetheless, also the two recent examples of the 2009 L'Aquila earthquake and 2012 Emilia earthquake confirmed and highlighted the vulnerability of cultural heritage structures to these types of events. In this paper the church of Santa Maria del Suffragio (Anime Sante) in L'Aquila is used as a benchmark for the experimental validation of a finite element model on the basis of the data gathered by the permanent structural health monitoring system installed on the building by IUAV in 2009. Structural health monitoring techniques have been largely applied to cultural Heritage buildings in recent times, mostly because of their non-destructive nature, and they have proven to be a valid tool in assessing the damage evolution and in characterising the global dynamic behaviour of the structure. In particular, a global sensitivity analysis technique has been applied to a finite element model. The model underwent a model updating procedure on the parameters chosen in the sensitivity analysis. The calibrated model is an invaluable tool in assessing the dynamic behaviour of the structure and may serve for several purposes

Sensitivity analysis of damaged monumental structures: the example of S. Maria del Suffragio in L'Aquila

In Italy, which accounts for an impressive number of architectural heritage sites, a large part of the territory is subject to seismic risk. Nonetheless, also the two recent examples of the 2009 L'Aquila earthquake and 2012 Emilia earthquake confirmed and highlighted the vulnerability of cultural heritage structures to these types of events. In this paper the church of Santa Maria del Suffragio (Anime Sante) in L'Aquila is used as a benchmark for the experimental validation of a finite element model on the basis of the data gathered by the permanent structural health monitoring system installed on the building by IUAV in 2009. Structural health monitoring techniques have been largely applied to cultural Heritage buildings in recent times, mostly because of their non-destructive nature, and they have proven to be a valid tool in assessing the damage evolution and in characterising the global dynamic behaviour of the structure. In particular, a global sensitivity analysis technique has been applied to a finite element model. The model underwent a model updating procedure on the parameters chosen in the sensitivity analysis. The calibrated model is an invaluable tool in assessing the dynamic behaviour of the structure and may serve for several purpose

Mezclas de cemento u otros ligantes aplicables a materiales y técnicas constructivas utilizando EPS molido y cascotes reciclados provenientes de residuos de construcción y demolición.

Desde el CEP desarrollamos materiales de construcción alternativos a los tradicionales para minimizar el impacto medioambiental generado por los residuos de construcción y demolición (RCD). El hormigón es el material de construcción más empleado en el mundo gracias a sus excelentes propiedades: durabilidad, resistencia y adaptación a moldes. Su uso produce grandes costos medioambientales: enorme cantidad de energía consumida para extracción, acarreo, fabricación y traslado de componentes. Esta investigación desarrolla distintas fórmulas utilizando EPS molido y cascotes fraccionados mezclados con ligantes apropiados para fabricar Materiales Constructivos durables, económicos, de bajo peso y con buena aislación térmica, reduciendo el impacto ambiental que produce la fabricación tradicional de bloques, ladrillos y revestimientos. El EPS es uno de los RCD de restos y de embalajes más perjudiciales al medio ambiente tanto por su volumen como por su prolongado tiempo de degradación, mientras que los cascotes son el principal RCD en nuestra área de trabajo. Estas mezclas pueden ser utilizadas para autoconstrucción y planes de vivienda contribuyendo a generar empleo, creando nuevos conocimientos y expandiendo la conciencia del cuidado Medioambiental. Los profesionales del Centro trabajamos junto con alumnos en programas de pasantías de investigación con crédito académico. Para la obtención de las citadas materias primas se han generado acuerdos con empresas generadoras, con planes logísticos para optimizar recorridos reduciendo consumo de combustible. De esta forma se evita que un considerable volumen de RCD llegue a vertederos y centros de enterramiento habituales y se reduce la potencialidad de su voladura tapando desagues pluviales. Se desarrollan por un lado mezclas aislantes en densidades de 500 a 600 kg/m3 que utilizadas en espesores habituales de construcción cumplen con las normas actuales de transmisión térmica. Estas mezclas incluyen cemento, residuos de EPS molidos pasantes por tamiz de 6mm y aditivios. Por otro lado se desarrollan mezclas en densidades de 900 a 1100 kg/m3 que utilizadas en espesores habituales cumplen con resistencia equivalente a la de ladrillos comunes. Estas mezclas incluyen cemento, residuos de EPS molidos pasantes por tamiz de 6mm, restos de cascotes molidos pasantes por tamiz de 20mm, arena y aditivos. Los moldes requeridos son fabricados a su vez con materiales reciclados de EPS

Indocyanine green (ICG) fluorescent cholangiography during laparoscopic cholecystectomy using RUBINA™ technology: preliminary experience in two pediatric surgery centers

Background: Recently, we reported the feasibility of indocyanine green (ICG) near-infrared fluorescence (NIRF) imaging to identify extrahepatic biliary anatomy during laparoscopic cholecystectomy (LC) in pediatric patients. This paper aimed to describe the use of a new technology, RUBINA™, to perform intra-operative ICG fluorescent cholangiography (FC) in pediatric LC. Methods: During the last year, ICG-FC was performed during LC using the new technology RUBINA™ in two pediatric surgery units. The ICG dosage was 0.35 mg/Kg and the median timing of administration was 15.6 h prior to surgery. Patient baseline, intra-operative details, rate of biliary anatomy identification, utilization ease, and surgical outcomes were assessed. Results: Thirteen patients (11 girls), with median age at surgery of 12.9 years, underwent LC using the new RUBINA™ technology. Six patients (46.1%) had associated comorbidities and five (38.5%) were practicing drug therapy. Pre-operative workup included ultrasound (n = 13) and cholangio-MRI (n = 5), excluding biliary and/or vascular anatomical anomalies. One patient needed conversion to open surgery and was excluded from the study. The median operative time was 96.9 min (range 55–180). Technical failure of intra-operative ICG-NIRF visualization occurred in 2/12 patients (16.7%). In the other cases, ICG-NIRF allowed to identify biliary/vascular anatomic anomalies in 4/12 (33.3%), including Moynihan's hump of the right hepatic artery (n = 1), supravescicular bile duct (n = 1), and short cystic duct (n = 2). No allergic or adverse reactions to ICG, post-operative complications, or reoperations were reported. Conclusion: Our preliminary experience suggested that the new RUBINA™ technology was very effective to perform ICG-FC during LC in pediatric patients. The advantages of this technology include the possibility to overlay the ICG-NIRF data onto the standard white light image and provide surgeons a constant fluorescence imaging of the target anatomy to assess position of critical biliary structures or presence of anatomical anomalies and safely perform the operation

Altered inflammasome machinery as a key player in the perpetuation of Rett syndrome oxinflammation

Rett syndrome (RTT) is a progressive neurodevelopmental disorder mainly caused by mutations in the X-linked MECP2 gene. RTT patients show multisystem disturbances associated with an oxinflammatory status. Inflammasomes are multi-protein complexes, responsible for host immune responses against pathogen infections and redox-related cellular stress. Assembly of NLRP3/ASC inflammasome triggers pro-caspase-1 activation, thus, resulting in IL-1β and IL-18 maturation. However, an aberrant activation of inflammasome system has been implicated in several human diseases. Our aim was to investigate the possible role of inflammasome in the chronic subclinical inflammatory condition typical of RTT, by analyzing this complex in basal and lipopolysaccharide (LPS)+ATP-stimulated primary fibroblasts, as well as in serum from RTT patients and healthy volunteers. RTT cells showed increased levels of nuclear p65 and ASC proteins, pro-IL-1β mRNA, and NLRP3/ASC interaction in basal condition, without any further response upon the LPS + ATP stimuli. Moreover, augmented levels of circulating ASC and IL-18 proteins were found in serum of RTT patients, which are likely able to amplify the inflammatory response. Taken together, our findings suggest that RTT patients exhibited a challenged inflammasome machinery at cellular and systemic level, which may contribute to the subclinical inflammatory state feedback observed in this pathology

Scaling up health knowledge at European level requires sharing integrated data: An approach for collection of database specification

Computerized health care databases have been widely described as an excellent opportunity for research. The availability of “big data” has brought about a wave of innovation in projects when conducting health services research. Most of the available secondary data sources are restricted to the geographical scope of a given country and present heterogeneous structure and content. Under the umbrella of the European Innovation Partnership on Active and Healthy Ageing, collaborative work conducted by the partners of the group on “adherence to prescription and medical plans” identified the use of observational and large-population databases to monitor medication-taking behavior in the elderly. This article describes the methodology used to gather the information from available databases among the Adherence Action Group partners with the aim of improving data sharing on a European level. A total of six databases belonging to three different European countries (Spain, Republic of Ireland, and Italy) were included in the analysis. Preliminary results suggest that there are some similarities. However, these results should be applied in different contexts and European countries, supporting the idea that large European studies should be designed in order to get the most of already available databases

Seminal plasma and prostaglandin E2 up-regulate fibroblast growth factor 2 expression in endometrial adenocarcinoma cells via E-series prostanoid-2 receptor-mediated transactivation of the epidermal growth factor receptor and extracellular signal-regulated kinase pathway

BACKGROUND: Prostaglandin E(2) (PGE(2)) has been shown to modulate angiogenesis and tumour progression via the E-series prostanoid-2 (EP2) receptor. Endometrial adenocarcinomas may be exposed to endogenous PGE(2) and exogenous PGE(2), present at high concentration in seminal plasma. METHODS: This study investigated fibroblast growth factor 2 (FGF2) mRNA expression and cell signalling in response to seminal plasma or PGE(2), using an endometrial adenocarcinoma (Ishikawa) cell line stably expressing the EP2 receptor (EP2 sense cells) and endometrial adenocarcinoma explants. RESULTS: Seminal plasma and PGE(2) induced a significant up-regulation of FGF2 expression in EP2 sense but not parental untransfected Ishikawa (wild-type) cells (P < 0.05). These effects were inhibited by co-treatment with EP2 receptor antagonist or inhibitors of protein kinase A, c-Src, epidermal growth factor receptor (EGFR) kinase or extracellular signal-regulated kinase (ERK) signalling. The treatment of EP2 sense cells with seminal plasma induced cAMP accumulation and phosphorylation of c-Src, EGFR kinase and ERK via the EP2 receptor. Finally, seminal plasma and PGE(2) significantly increased FGF2 mRNA expression in endometrial adenocarcinoma tissue explants via the EP2 receptor (P < 0.05). CONCLUSIONS: Seminal plasma and PGE(2) can similarly activate FGF2 expression and EP2 receptor signalling in endometrial adenocarcinoma cells. These data highlight the potential for seminal plasma exposure to facilitate tumorigenesis–angiogenesis in endometrial adenocarcinomas in vivo

Human papillomavirus 9-valent vaccine for cancer prevention: a systematic review of the available evidence.

In 2014, the Food and Drug Administration approved a new human papillomavirus 9-valent vaccine (9vHPV), targeting nine HPV types: HPV types 6, 11, 16, and 18, which are also targeted by the quadrivalent HPV vaccine (qHPV), plus five additional high cancer risk HPV types (HPV types 31, 33, 45, 52, and 58). The aim of the current study was to systematically retrieve, qualitatively and quantitatively pool, as well as critically appraise all available evidence on 9vHPV from randomized controlled trials (RCTs). We conducted a systematic review of the literature on 9vHPV efficacy, immunogenicity and safety, as well as a systematic search of registered, completed, and ongoing RCTs. We retrieved and screened 227 records for eligibility. A total of 10 publications reported on RCTs' results on 9vHPV and were included in the review. Sixteen RCTs on 9vHPV have been registered on RCT registries. There is evidence that 9vHPV generated a response to HPV types 6, 11, 16 and 18 that was non-inferior to qHPV. Vaccine efficacy against five additional HPV type-related diseases was directly assessed on females aged 16-26 years (risk reduction against high-grade cervical, vulvar or vaginal disease = 96·7%, 95% CI 80·9%-99·8%). Bridging efficacy was demonstrated for males and females aged 9-15 years and males aged 16-26 years (the lower bound of the 95% CIs of both the geometric mean titer ratio and difference in seroconversion rates meeting the criteria for non-inferiority for all HPV types). Overall, 9vHPV has been proved to be safe and well tolerated. Other RCTs addressed: 9vHPV co-administration with other vaccines, 9vHPV administration in subjects that previously received qHPV and 9vHPV efficacy in regimens containing fewer than three doses. The inclusion of additional HPV types in 9vHPV offers great potential to expand protection against HPV infection. However, the impact of 9vHPV on reducing the global burden of HPV-related disease will greatly depend on vaccine uptake, coverage, availability, and affordability