Getting Rid of e-Books in the SFX Knowledge Base

The poster provides an overview on e-book resources available in the MPG, introduces the MPG e-Book Catalog and discusses the dynamic integration of e-Book holdings into the service menu of the MPG/SFX link resolver

Compensation in resting metabolism for experimentally increased activity

To study zebra finch allocation of energy to day and night at two different workloads, we assessed the daily energy turnover from: (1) metabolizable energy of the food, and (2) doubly-labeled water. In both experiments we imposed two levels of activity on captive zebra finches (Taeniopygia guttata), by applying different computer-controlled workload schedules. A low workload required 20 hops, and a high workload 40 hops to obtain 10 s access to food. In experiment 1, we further measured nocturnal energy expenditure by overnight oxygen consumption. From experiment 2 we derived an estimate of the costs of hopping activity, from inter-individual association of daily amount of hopping and daily energy expenditure. Surprisingly, the daily energy budget was, on average, reduced slightly when birds were subjected to a high workload. Since hopping activity was 50% higher during the high workload than during the low workload, the birds apparently compensated, even over-compensated, for the increased energetic demands of activity. Nocturnal energy expenditure was indeed reduced for the high workload, which was largely due to a reduction in resting metabolic rate. Economizing on energy was more than could have been accomplished by a reduction in mass alone, and we discuss the occurrence and potential mechanisms of physiological compensation. The amount of energy saved during the night did account for part of the total amount of energy saved. We surmise that the strategy of energetic compensation observed during the night was extended into the inactive hours of the day

Imidazole-imidazole hydrogen bonding in the pH-sensing histidine side chains of influenza A M2.

The arrangement of histidine side chains in influenza A M2 tetramer determines their pKa values, which define pH-controlled proton conduction critical to the virus lifecycle. Both water-associated and hydrogen-bonded imidazole-imidazolium histidine quaternary structures have been proposed, based on crystal structures and NMR chemical shifts, respectively. Here we show, using the conduction domain construct of M2 in lipid bilayers, that the imidazole rings are hydrogen bonded even at a pH of 7.8 in the neutral charge state. An intermolecular 8.9 ± 0.3 Hz 2hJNN hydrogen bond is observed between H37 Nε and Nδ recorded in a fully protonated sample with 100 kHz magic-angle spinning. This interaction could not be detected in the drug-bound sample

Комбинированная кинезотерапия в лечении больных коксартрозом

Вивчені результати лікування 54 пацієнтів з коксартрозом. Включення комплексного лікування даного контингенту пацієнтів комбінованої кінезотерапії методикою Євмінова дозволяє підвищити ефективність проведеної терапії.This article studied results of treatment of 54 patients with coxarthrosis. Upon inclusion of the combined kinesitherapy under Evminov method into the complex treatment of the mentioned group of patients it became possible to increase the effectiveness of the whole therapy

Metabolic engineering with ATP-citrate lyase and nitrogen source supplementation improves itaconic acid production in Aspergillus niger

FWN – Publicaties zonder aanstelling Universiteit Leide

A litmus test for classifying recognition mechanisms of transiently binding proteins

Partner recognition in protein binding is critical for all biological functions, and yet, delineating its mechanism is challenging, especially when recognition happens within microseconds. We present a theoretical and experimental framework based on straight-forward nuclear magnetic resonance relaxation dispersion measurements to investigate protein binding mechanisms on sub-millisecond timescales, which are beyond the reach of standard rapid-mixing experiments. This framework predicts that conformational selection prevails on ubiquitin’s paradigmatic interaction with an SH3 (Src-homology 3) domain. By contrast, the SH3 domain recognizes ubiquitin in a two-state binding process. Subsequent molecular dynamics simulations and Markov state modeling reveal that the ubiquitin conformation selected for binding exhibits a characteristically extended C-terminus. Our framework is robust and expandable for implementation in other binding scenarios with the potential to show that conformational selection might be the design principle of the hubs in protein interaction networks

Communicating Processes with Data for Supervisory Coordination

We employ supervisory controllers to safely coordinate high-level discrete(-event) behavior of distributed components of complex systems. Supervisory controllers observe discrete-event system behavior, make a decision on allowed activities, and communicate the control signals to the involved parties. Models of the supervisory controllers can be automatically synthesized based on formal models of the system components and a formalization of the safe coordination (control) requirements. Based on the obtained models, code generation can be used to implement the supervisory controllers in software, on a PLC, or an embedded (micro)processor. In this article, we develop a process theory with data that supports a model-based systems engineering framework for supervisory coordination. We employ communication to distinguish between the different flows of information, i.e., observation and supervision, whereas we employ data to specify the coordination requirements more compactly, and to increase the expressivity of the framework. To illustrate the framework, we remodel an industrial case study involving coordination of maintenance procedures of a printing process of a high-tech Oce printer.Comment: In Proceedings FOCLASA 2012, arXiv:1208.432