17 research outputs found
The effect of agitation speed, enzyme loading and substrate concentration on enzymatic hydrolysis of cellulose from brewer’s spent grain
Brewer’s spent grain components (cellulose,
hemicellulose and lignin) were fractionated in a
two-step chemical pretreatment process using dilute
sulfuric acid and sodium hydroxide solutions. The
cellulose pulp produced was hydrolyzed with a
cellulolytic complex, Celluclast 1.5 L, at 45 ºC to
convert the cellulose into glucose. Several conditions
were examined: agitation speed (100, 150 and
200 rpm), enzyme loading (5, 25 and 45 FPU/g
substrate), and substrate concentration (2, 5 and 8%
w/v), according to a 2 3 full factorial design aiming to
maximize the glucose yield. The obtained results
were interpreted by analysis of variance and response
surface methodology. The optimal conditions for
enzymatic hydrolysis of brewer’s spent grain were
identified as 100 rpm, 45 FPU/g and 2% w/v substrate.
Under these conditions, a glucose yield of
93.1% and a cellulose conversion (into glucose and
cellobiose) of 99.4% was achieved. The easiness of
glucose release from BSG makes this substrate a raw material with great potential to be used in bioconversion
processes.Coordenação de Aperfeiçoamento de Pessoal de NÃvel
Superior (CAPES)Fundação de Amparo
à Pesquisa do Estado de São Paulo), Brazil. Novozymes ( FAPESP )Conselho Nacional de Desenvolvimento
CientÃfico e Tecnológico (CNPq