Purification and characterization of chitinase from Alcaligenes faecalis AU02 by utilizing marine wastes and its antioxidant activity

Marine waste is an abundant renewable source for the recovery of several value added metabolites with potential industrial applications. This study describes the production of chitinase on marine waste, with the subsequent use of the same marine waste for the extraction of antioxidants. A chitinase-producing bacterium isolated from seafood effluent was identified as Alcaligenes faecalis AU02. Optimal chitinase production was obtained in culture conditions of 37°C for 72 h in 100 ml medium containing 1% shrimp and crab shell powder (1:1) (w/v), 0.1% K2HPO4, and 0.05% MgSO4·7H2O. The molecular weight of chitinase was determined by SDS-PAGE to be 36 kDa. The optimum pH, temperature, pH stability, and thermal stability of chitinase were about 8, 37°C, 5–12, and 40–80°C, respectively. The antioxidant activity of A. faecalis AU02 culture supernatant was determined through scavenging ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH) as 84%, and the antioxidant compound was characterized by TLC and its FT-IR spectrum. The present study proposed that marine wastes can be utilized to generate a high-value-added product and that pharmacological studies can extend its use to the field of medicine

Effect of graphene and carbon nanotubes on the negative active materials of lead acid batteries operating under high-rate partial-state-of-charge operation

Graphene and CNTs have different impacts on the structural and electrochemical properties of lead acid battery NAM under HRPSoC cycling.</p

Mesoporous silica based reservoir for the active protection of mild steel in an aggressive chloride ion environment

Spherical mesoporous silica (m-SiO2) with well-ordered pores was synthesized by a modified Stöber method using CTAB micelles.</p

Effect of using sonicated sulphuric acid as an electrolyte in a lead acid battery

The consequences of adding sonicated sulphuric acid as an electrolyte in a lead acid battery (LAB) have been investigated.</p