Effect of temperature and relative humidity on the development times and survival of Synopsyllus fonquerniei and Xenopsylla cheopis, the flea vectors of plague in Madagascar

Acknowledgements We would like to thank Dr Lila Rahalison and Jocelyn Ratovonjato for their advice and help during the experiment. We are grateful to the staff of the Plague Unit and the Medical Entomology Unit at the Institut Pasteur de Madagascar, particularly Dr Nohal Elissa. Without their expertise this study would not have been possible. Sincere thanks to Mr Tojo Ramihangihajason for his technical assistance. We are indebted to the Institut Pasteur de Madagascar for an internal grant which facilitated additional laboratory research. Two Wellcome Trust fellowships supported ST during this work (081705 and 095171).Peer reviewedPublisher PD

Dietary supplementation of essential oils in dairy cows: evidence for stimulatory effects on nutrient absorption

Results of recent in vitro experiments suggest that essential oils (EO) may not only influence ruminal fermentation but also modulate the absorption of cations like Na+, Ca2+ and NH4+ across ruminal epithelia of cattle and sheep through direct interaction with epithelial transport proteins, such as those of the transient receptor potential family. The aim of the current study was to examine this hypothesis by testing the effect of a blend of essential oils (BEO) on cation status and feed efficiency in lactating dairy cows. In the experiment, 72 dairy cows in mid-to-end lactation were divided into two groups of 36 animals each and fed the same mixed ration with or without addition of BEO in a 2×2 cross-over design. Feed intake, milk yield and composition, plasma and urine samples were monitored. Feeding BEO elevated milk yield, milk fat and protein yield as well as feed efficiency, whereas urea levels in plasma and milk decreased. In addition, plasma calcium levels increased significantly upon BEO supplementation, supporting the hypothesis that enhanced cation absorption might contribute to the beneficial effects of these EO

Rapid evolution of protein kinase PKR alters sensitivity to viral inhibitors.

Protein kinase PKR (also known as EIF2AK2) is activated during viral infection and phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF2), leading to inhibition of translation and viral replication. We report fast evolution of the PKR kinase domain in vertebrates, coupled with positive selection of specific sites. Substitution of positively selected residues in human PKR with residues found in related species altered sensitivity to PKR inhibitors from different poxviruses. Species-specific differences in sensitivity to poxviral pseudosubstrate inhibitors were identified between human and mouse PKR, and these differences were traced to positively selected residues near the eIF2alpha binding site. Our findings indicate how an antiviral protein evolved to evade viral inhibition while maintaining its primary function. Moreover, the identified species-specific differences in the susceptibility to viral inhibitors have important implications for studying human infections in nonhuman model systems

Effective short-range interaction for spin-singlet P-wave nucleon-nucleon scattering

Distorted-wave methods are used to remove the effects of one- and two-pion exchange up to order Q^3 from the empirical 1P1 phase shift. The one divergence that arises can be renormalised using an order-Q^2 counterterm which is provided by the (Weinberg) power counting appropriate to the effective field theory for this channel. The residual interaction is used to estimate the scale of the underlying physics.Comment: 4 pages, 3 figures (pdf

KETERAMPILAN MENULIS PARAGRAF DESKRIPTIF BAHASA JERMAN DENGAN METODE ESTAFET WRITING BERMEDIA GAMBAR SISWA KELAS X SMAN 1 GEDANGAN

Writing is one of the productive aspects that produces a language. Writing skills should be trained regularly so, can produce a good and correct writing. In the process of German learning, not all students are able to write good and correctly. Particularly in writing German essay, students often have difficulty writing a sentence. For that we need an effective method and effective strategy that is Estafet Writing method using picture media. The application of the Estafet writing method and using picture media as a supporter can involve all students to share their knowledge and ideas and then develop their imagination through a written. The formulation of this research problem is: how the process of applying the method of Estafet Writing using picture media in writing skill learning of German language class X SMAN 1 Gedangan ?. The purpose of this research is to describe the process of applying the Estafet writing method using picture media in writing skills learning German opus writing student class X.      This type of this research is a qualitative research. Data collection techniques use observation sheets (observation), documentation and tests to support the process of applying the method of Estafet Writing using picture media. The study was conducted on March 24 - 7 April 2017, three times meeting and followed by 34 students.             Based on the result of the research, it can be concluded that the method of Estafet Writing using picture media is effective applied in learning the writing skill of German language paragraph. This can be seen from the successful implementation of the method of Estafet Writing using picture media shown through observation data. In addition, the more attention of students of SMAN 1 Gedangan with the method of Estafet Writing using picture media.                                                            Keywords       : Estafet Writing method, Writingskill

Further Characterization of the Mitigation of Radiation Lethality by Protective Wounding

There continues to be a major effort in the United States to develop mitigators for the treatment of mass casualties that received high-intensity acute ionizing radiation exposures from the detonation of an improvised nuclear device during a radiological terrorist attack. The ideal countermeasure should be effective when administered after exposure, and over a wide range of absorbed doses. We have previously shown that the administration of a subcutaneous incision of a defined length, if administered within minutes after irradiation, protected young adult female C57BL/6 mice against radiation-induced lethality, and increased survival after total-body exposure to an LD50/30 X-ray dose from 50% to over 90%. We refer to this approach as "protective wounding". In this article, we report on our efforts to further optimize, characterize and demonstrate the validity of the protective wounding response by comparing the response of female and male mice, varying the radiation dose, the size of the wound, and the timing of wounding with respect to administration of the radiation dose. Both male and female mice that received a subcutaneous incision after irradiation were significantly protected from radiation lethality. We observed that the extent of protection against lethality after an LD50/30 X-ray dose was independent of the size of the subcutaneous cut, and that a 3 mm subcutaneous incision is effective at enhancing the survival of mice exposed to a broad range of radiation doses (LD15-LD100). Over the range of 6.2-6.7 Gy, the increase in survival observed in mice that received an incision was associated with an enhanced recovery of hematopoiesis. The enhanced rate of recovery of hematopoiesis was preceded by an increase in the production of a select group of cytokines. Thus, a thorough knowledge of the timing of the cytokine cascade after wounding could aid in the development of novel pharmacological radiation countermeasures that can be administered several days after the actual radiation exposure