Activation of Ciona sperm motility: phosphorylation of dynein polypeptides and effects of a tyrosine kinase inhibitor

A high molecular mass dynein ATPase polypeptide and a 18–20 kDa dynein light chain of Ciona sperm flagella are phosphorylated during in vivo activation of motility or in vitro activation of motility by incubation with cyclic AMP. A similar level of phosphorylation of these proteins is obtained by incubation of washed, demembranated spermatozoa with catalytic subunit of cyclic AMP-dependent protein kinase, under conditions where there is no activation of motility until a supernatant component is added. Therefore, phosphorylation of these dynein polypeptides is not sufficient for activation of motility. Activation of motility in vitro by incubation with cyclic AMP can be completely inhibited by a random copolymer of glutamate and tyrosine that inhibits tyrosine kinase activity. Under these conditions, much of the protein phosphorylation associated with activation of motility is also inhibited. These new results suggest that regulation of motility of these spermatozoa may involve a multicomponent kinase cascade rather than a simple phosphorylation of a protein ‘switch’ by the cyclic AMP-dependent kinase. A 53 kDa axonemal phosphoprotein band, identified as band M1, shows the strongest correlation with activation of motility in these experiments

Ridge Fusion in Statistical Learning

We propose a penalized likelihood method to jointly estimate multiple precision matrices for use in quadratic discriminant analysis and model based clustering. A ridge penalty and a ridge fusion penalty are used to introduce shrinkage and promote similarity between precision matrix estimates. Block-wise coordinate descent is used for optimization, and validation likelihood is used for tuning parameter selection. Our method is applied in quadratic discriminant analysis and semi-supervised model based clustering.Comment: 24 pages and 9 tables, 3 figure

Non-equilibrium thermodynamics in sheared hard-sphere materials

We combine the shear-transformation-zone (STZ) theory of amorphous plasticity with Edwards' statistical theory of granular materials to describe shear flow in a disordered system of thermalized hard spheres. The equations of motion for this system are developed within a statistical thermodynamic framework analogous to that which has been used in the analysis of molecular glasses. For hard spheres, the system volume $V$ replaces the internal energy $U$ as a function of entropy $S$ in conventional statistical mechanics. In place of the effective temperature, the compactivity $X = \partial V / \partial S$ characterizes the internal state of disorder. We derive the STZ equations of motion for a granular material accordingly, and predict the strain rate as a function of the ratio of the shear stress to the pressure for different values of a dimensionless, temperature-like variable near a jamming transition. We use a simplified version of our theory to interpret numerical simulations by Haxton, Schmiedeberg and Liu, and in this way are able to obtain useful insights about internal rate factors and relations between jamming and glass transitions.Comment: 9 pages, 6 figure

A Relativistic Quaternionic Wave Equation

We study a one-component quaternionic wave equation which is relativistically covariant. Bi-linear forms include a conserved 4-current and an antisymmetric second rank tensor. Waves propagate within the light-cone and there is a conserved quantity which looks like helicity. The principle of superposition is retained in a slightly altered manner. External potentials can be introduced in a way that allows for gauge invariance. There are some results for scattering theory and for two-particle wavefunctions as well as the beginnings of second quantization. However, we are unable to find a suitable Lagrangian or an energy-momentum tensor.Comment: 19 pages; minor corrections in Section 11 and Appendix

Polarization spectroscopy of an excited state transition.

We demonstrate polarization spectroscopy of an excited state transition in room-temperature cesium vapor. An anisotropy induced by a circularly polarized pump beam on the D2 transition is observed using a weak probe on the 6P3/2→7S1/2 transition. At high pump power, a subfeature due to Autler-Townes splitting is observed that theoretical modeling shows is enhanced by Doppler averaging. Polarization spectroscopy provides a simple modulation–free signal suitable for laser frequency stabilization to excited state transitions

Time (in)dependence in general relativity

We clarify the conditions for Birkhoff's theorem, that is, time-independence in general relativity. We work primarily at the linearized level where guidance from electrodynamics is particularly useful. As a bonus, we also derive the equivalence principle. The basic time-independent solutions due to Schwarzschild and Kerr provide concrete illustrations of the theorem. Only familiarity with Maxwell's equations and tensor analysis is required.Comment: Revised version of originally titled "Kinder Kerr", to appear in American Journal of Physic