15 research outputs found
The NS1 Glycoprotein Can Generate Dramatic Antibody-Enhanced Dengue Viral Replication in Normal Out-Bred Mice Resulting in Lethal Multi-Organ Disease
Antibody-enhanced replication (AER) of dengue type-2 virus (DENV-2) strains and production of antibody-enhanced disease (AED) was tested in out-bred mice. Polyclonal antibodies (PAbs) generated against the nonstructural-1 (NS1) glycoprotein candidate vaccine of the New Guinea-C (NG-C) or NSx strains reacted strongly and weakly with these antigens, respectively. These PAbs contained the IgG2a subclass, which cross-reacted with the virion-associated envelope (E) glycoprotein of the DENV-2 NSx strain, suggesting that they could generate its AER via all mouse Fcγ-receptor classes. Indeed, when these mice were challenged with a low dose (<0.5 LD50) of the DENV-2 NSx strain, but not the NG-C strain, they all generated dramatic and lethal DENV-2 AER/AED. These AER/AED mice developed life-threatening acute respiratory distress syndrome (ARDS), displayed by diffuse alveolar damage (DAD) resulting from i) dramatic interstitial alveolar septa-thickening with mononuclear cells, ii) some hyperplasia of alveolar type-II pneumocytes, iii) copious intra-alveolar protein secretion, iv) some hyaline membrane-covered alveolar walls, and v) DENV-2 antigen-positive alveolar macrophages. These mice also developed meningo-encephalitis, with greater than 90,000-fold DENV-2 AER titers in microglial cells located throughout their brain parenchyma, some of which formed nodules around dead neurons. Their spleens contained infiltrated megakaryocytes with DENV-2 antigen-positive red-pulp macrophages, while their livers displayed extensive necrosis, apoptosis and macro- and micro-steatosis, with DENV-2 antigen-positive Kuppfer cells and hepatocytes. Their infections were confirmed by DENV-2 isolations from their lungs, spleens and livers. These findings accord with those reported in fatal human “severe dengue” cases. This DENV-2 AER/AED was blocked by high concentrations of only the NG-C NS1 glycoprotein. These results imply a potential hazard of DENV NS1 glycoprotein-based vaccines, particularly against DENV strains that contain multiple mutations or genetic recombination within or between their DENV E and NS1 glycoprotein-encoding genes. The model provides potential for assessing DENV strain pathogenicity and anti-DENV therapies in normal mice
Healing through culturally embedded practice: an investigation of counsellors’ and clients’ experiences of Buddhist Counselling in Thailand
This thesis is concerned with an exploration of counsellors’ and clients’ lived experiences of
Buddhist Counselling, an indigenous Buddhist-based counselling approach in Thailand. Over
the past decade, Buddhist Counselling has received a growing interest from Thai counselling
trainees and practitioners, and it has also expanded to serve Thai people in various settings.
Research on Buddhist Counselling is very limited and most of the existing studies in the
field have focused on measuring the effectiveness of the approach. While these studies have
consistently indicated the positive effects of Buddhist Counselling on psychological
improvement across several population groups, the significant questions of how Buddhist
Counselling brings about such outcome and how it is experienced are still largely
unanswered. Moreover, existing research is concentrated much more on clients’ views than
counsellors’ views, although counsellors’ views of their counselling practice can also serve as
a knowledge base of the field. This thesis thus sets out to contribute to rectifying this
omission by exploring Buddhist Counselling from the perspectives of both counsellors and
clients.
The thesis is based on two qualitative studies. The first study addressed Buddhist
Counselling from the perspective of five counsellors through a focus group and semi-structured
interviews. The second study explored Buddhist Counselling from the perspective
of three clients, using two semi-structured interviews with each of them. All data received
were analysed using interpretative phenomenological analysis (IPA).
The study reveals counsellors’ and clients’ overall positive experience of engaging in
Buddhist Counselling. Central to the accounts of the counsellors are the following
perceptions: that their practice of Buddhist Counselling is culturally congruent with the
existing values and beliefs of both themselves and their clients; that their personal and
professional congruence is key to their therapeutic efficacy; and that they enhance such
congruence through their application of Buddhist ideas and practices in their daily lives. Key
to the clients’ accounts is their emphasis on the significant roles of the counsellors’ Buddhist
ideas and personal qualities, and of their religious practices in facilitating healing and change.
Key shared findings from both studies reveal that the participants’ accounts of their cultural
background and their experiences of Buddhist Counselling are intertwined. Adopting
hermeneutics to address this intertwinement, I reveal the cultural and moral dimensions
underlying the practice of Buddhist Counselling. Based on such revelation, I suggest that
Buddhist Counselling in particular, as well as psychotherapy in general, should be better
understood as a historically situated, culturally bound, and morally constituted activity of
people who are concerned with improving the quality of their lives and their community,
rather than the transcultural and merely relational work of morally-neutral practitioners