TOOTH SENSITIVITY AMONG RESIDENTIAL UNIVERSITY STUDENTS IN CHENNAI

ABSTRACTObjective: To assess the experience of TS of residential university students from different universities in Chennai.Methods: Students of different colleges were given questionnaires on TS. The answered questionnaires were then analyzed in SPSS online softwareand the results were foundResults: From the data, it is infered that the knowledge of treatment for TS isn't well-known among students and improper brushing techniques andsoft drink consumption are main reasons for TS.Conclusion: From the data, one can infer that TS has becomeNo quite prevalent in today's society and with less awareness on its treatment. Thepeople should be taught to maintain their oral hygiene, and proper brushing techniques should be taught and diet should be altered according tohealth. Soft drinks should be avoided to the highest extent and treatment for sensitivity should be taken promptly.Keywords: Dentin, sensitivity, students

PRESCRIPTION PATTERN ANALYSIS OF ANTI-INFLAMMATORY DRUGS IN GENERAL MEDICINE AND SURGERY DEPARTMENT AT A TERTIARY CARE HOSPITAL

Objective: The objective of this research was to evaluate and analyze the prescription pattern of anti-inflammatory drugs given in the general medicine and surgery department at a tertiary care hospital.Methods: The entire study was planned to be carried out for a period of six months. Total 84 patients were included in this study and was carried out in a 300 bedded tertiary care hospital. The patients admitted to the general medicine and surgery department were included in this study, of which patients given with anti-inflammatory drugs were recorded and analyzed for prescribing practice and rational use.Results: The majority of the patients were admitted to general medicine ward rather than surgery ward, and the non-steroidal anti-inflammatory drugs (NSAIDs) were highly prescribed to the age group of 41-60 y. And about 88.89 % of non-NSAID injection, 11.11% of NSAID injection were prescribed. The prescribed drugs were mostly given by generic names (81.52 %) and least by brand name (18.48 %).Conclusion: This results obtained in this research work will be helpful to increase awareness of the limitations and difficulties on the translation of these recommendations into clinical practice, but also to stimulate the creation of strategies or tools to increase the appropriate therapy.Keywords: Prescription pattern, NSAIDs, Tertiary care hospital, Non-steroidal anti-inflammatory drugs, Rational use of drug

THE EFFECT OF METFORMIN ON NON-ENZYMATIC GLYCOSYLATION OF RECOMBINANT HUMAN SERUM ALBUMIN

Objective: The present research work was aimed to observe the effect of Metformin hydrochloride (MET) on non-enzymatic glucosylation of recombinant human serum albumin (rHSA).Methods: Albumin was incubated at 37 ° in 10 mM phosphate buffer (pH 7.4) using 0.2% sodium azide for 7 d with different concentrations of D-(+)-glucose and MET. Incorporation of D-(+)-glucose (5-12 mM) into rHSA was calculated in the presence of metformin hydrochloride (1-5 µg/ml) following extensive dialysis of the incubation mixture. The binding experiments were exercised at respective plasma concentration of rHSA, D-(+)-glucose and MET in corresponding to that of diabetic and non-diabetic population. Glycosylation of rHSA was quantified using 2-thiobarbituric acid (2-TBA). Whereas, metformin hydrochloride was quantified using a validated LC-MS/MS method with negative ion electrospray ionization using multiple reactions monitoring (MRM) mode. MET was eluted isocratically on X-Bridge BEH Phenyl column with a mobile phase consisting of mixture of water (containing 50 mM ammonium acetate) and acetonitrile in a ratio of 70:30.Results: The weighted (1/X2) calibration curve from 20 to 10, 000 ng/ml was employed for the calculation ofMET in sample with line equation of Y= 0.0020584x+0.0037475. The binding pattern ofMET to rHSA, was specific and pH dependent, possibly due to a change in ionization state of MET and change in conformation of rHSA during the binding process.Conclusion: All the described parameters were in accordance to the FDA Guideline (inside 85-115% for the accuracy and less than 15% for the precision), thus it can be concluded that the bioanalytical method is were fully validated as per USFDA guideline. These experiments showed the affinity ofMETtoward the rHSA proved to be higher than its affinity toward the glucose

A Novel Approach for the Design of Controllers with Interleaved Boost Converter

Themomentous increase in exigency of electric energy is forced us to find various source of generations like solar, wind, hydraulic and clean energy like fuel cell. Low cost, high efficiency, compactness, portability and environmentally clean energy has enhanced the research on fuel cell. One of the factor which affects the life time of the fuel cell is ripple content presents in the current. In order to supply high voltage and low current applications, fuel cell is used to integrate with boost converter. But to improve the performance and lifetime of fuel cell, interleaved boost converter is employed. Also to supply for constant load the output voltage has to be maintained at a particular value. To attain this controllers are exploited. The conventional PI controller and smith predictive controller are designed for fuel cell fed interleaved boost converter in order to track the output voltage and to improve the transient time.The results depict the validity of the design procedure and the potential of the proposed method