Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)

We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and metabolic engineering studies in Streptomyces.Peer reviewe

Strongyloid hyperinfection in a patient with immunocompromised chronic kidney disease

Strongyloid hyperinfection is seen in immunocompromised individuals with underlying lung disease. The use of immunosuppressive drugs is an important risk factor. We report a case of IgA nephropathy with crescent, started on glucocorticoid and mycophenolate mofetil. He presented with bilateral lung opacities with breathlessness. As the breathlessness was not improving, despite adequate ultrafiltration, bronchoscopy was done. Bronchoalveolar lavage fluid examination showed the presence of strongyloid larvae, which was later demonstrated in stool also. He responded to antihelminthic treatment with disappearance of larvae from stool but later developed secondary bacterial infections

Dc Motor Speed Contol Techniques

This paper gives the idea about different techniques used for speed controlling of dc motor. Even the maintenance cost is higher than the Induction motor dc motor is widely used in industry. These controllers are used in different areas like processor control, manufacturing, automation, aerospace, etc. PID parameters have great effect on stability and performance of control system. Because of its salient features like reliability, wide range of torque-speed control range, efficiency, high starting torque DC motors are used in wide range. Speed control of DC motor is either done manually by the operator or by means of some automatic control devices. PWM based speed control system consist of electronic components like integrated circuits, sensors, etc