HIGH-DENSITY GREEN PHOTONS EFFECTS ON NaCl SOLUTIONS DETECTED BY RED BLOOD CELLS MEMBRANES

This paper presents a new technique for investigating the modifications induced by highdensity green light [GL] on water in NaCl solutions. Solutions of 0,45 g% and 0,9 g%, irradiated with green light (λ=527 nm, intensity 3·10 5 Lx) were used. As a receptor for measuring the irradiation effect we used red blood cell (RBC) permeability in hypotonic media, the so-called osmotic shock. After RBC lysis in hypotonic medium, the released hemoglobin was spectrophotometrically determined at λ=550 nm. The values of the osmotic shock for the samples obtained with GL-irradiated NaCl solutions were significantly lower than the values obtained with non-irradiated controls. This may indicate that the penetration of water inside the membranes canaliculi is hindered. This new type of result was complemented by chronoamperometry and impedance spectroscopy determinations. The current density of the irradiated solution decreases from the value of 29.5 µA·cm -2 in the control to 17.74 µA·cm -2 in the irradiated sample with a corresponding decrease of ionic mobility. The impedance value of the GL irradiated NaCl solutions were significantly lower than the control values, thus correlating well with the data recorded by chronoamperometry. All these data may indicate large water cluster formation through GL irradiation which are beyond the cellular aquaporine channels capacity. A similar process is identified by using red light, as well as blue light, though with a much smaller output

Green light effects on biological systems: a new biophysical phenomenon

This paper reports a new phenomenon connected with the influence of green light (GL) on biological systems. Our experiments have revealed an antioxidant effect of GL on cells subjected to lethal doses of UV at the cellular level and a protective effect of GL on DNA denatured by UV, coupled with a structural modification of DNA macromolecules under GL irradiation, at the molecular level. Mouse melanocyte cultures are subjected to UV irradiations with L50 fluxes of 16.0 J m − 2 s − 1. GL is obtained from a strontium aluminate pigment, which emits GL under UV activation. Cells grown in GL, prior to UV irradiation, present a clear surprising protective effect with surviving values close to the controls. A GL antioxidant effect is suggested to be mediated through GL influence on cellular water cluster dynamics. To test this hypothesis, reactive oxygen species (ROS) are determined in cell cultures. The results revealed a decrease of cellular ROS generation in the UV-irradiated samples protected by a previous 24 h of GL irradiation. At the DNA level, the same type of GL protection against UV damage is recorded by gel electrophoresis and by UV spectroscopy of the irradiated DNA molecules. Two physical methods, impedance spectroscopy and chronoamperometry, have revealed at the level of GL-irradiated DNA molecules spectral modifications that correlate with the UV spectroscopy results. The interaction between the chargeless photons and the field of water molecules from the cellular compartments is discussed in relation with the new field of macroscopic quantum coherence phenomena