1 research outputs found
Aqueous extracts from dietary supplements influence the production of inflammatory cytokines in immortalized and primary T lymphocytes
<p>Abstract</p> <p>Background</p> <p>Congaplex<sup>® </sup>and Immuplex<sup>® </sup>are dietary supplements that have been traditionally used to support immune system function. The purpose of these experiments was to determine whether Congaplex<sup>® </sup>and Immuplex<sup>® </sup>affect immune function using primary and immortalized T lymphocytes.</p> <p>Methods</p> <p>Immortalized CEM and Jurkat T lymphocytes and primary peripheral mononuclear blood cells (PBMCs) were treated with the aqueous extracts from Congaplex<sup>® </sup>and Immuplex<sup>® </sup>to determine the effects of these products on cytokine production in activated T lymphocytes.</p> <p>Results</p> <p>Congaplex<sup>® </sup>enhanced phytohemagglutinin/phorbol 12-myristate 13-acetate (PHA/PMA) stimulation of both CEM and Jurkat cells as measured by the production of cytokines, while Immuplex<sup>® </sup>suppressed PHA/PMA-induced production of cytokines, with the exception of interleukin (IL)-8 which was enhanced by Immuplex<sup>®</sup>. <it>In vitro </it>treatment of PBMCs from 10 healthy donors with Congaplex<sup>® </sup>or Immuplex<sup>® </sup>decreased PHA-stimulated production of interferon (IFN)-γ but increased the production of IL-13.</p> <p>Conclusions</p> <p>While the effects of Congaplex<sup>® </sup>and Immuplex<sup>® </sup>differed in these two models, these data demonstrate that the aqueous extracts from these two dietary supplements can affect the inflammatory response of T lymphocytes.</p