Radiations and male fertility

During recent years, an increasing percentage of male infertility has to be attributed to an array of environmental, health and lifestyle factors. Male infertility is likely to be affected by the intense exposure to heat and extreme exposure to pesticides, radiations, radioactivity and other hazardous substances. We are surrounded by several types of ionizing and non-ionizing radiations and both have recognized causative effects on spermatogenesis. Since it is impossible to cover all types of radiation sources and their biological effects under a single title, this review is focusing on radiation deriving from cell phones, laptops, Wi-Fi and microwave ovens, as these are the most common sources of non-ionizing radiations, which may contribute to the cause of infertility by exploring the effect of exposure to radiofrequency radiations on the male fertility pattern. From currently available studies it is clear that radiofrequency electromagnetic fields (RF-EMF) have deleterious effects on sperm parameters (like sperm count, morphology, motility), affects the role of kinases in cellular metabolism and the endocrine system, and produces genotoxicity, genomic instability and oxidative stress. This is followed with protective measures for these radiations and future recommendations. The study concludes that the RF-EMF may induce oxidative stress with an increased level of reactive oxygen species, which may lead to infertility. This has been concluded based on available evidences from in vitro and in vivo studies suggesting that RF-EMF exposure negatively affects sperm quality

Integrated impedance scanner in selected biomeasurement applications - control circuit

In the paper the results of preliminary experiments concerning bioelectrical impedance measurements using integrated impedance scanner AD5933 (Analog Devices) are described. Results of performed simulations are also presented. Design of a control circuit based on ARM microprocessor is described. Possible applications of the bioelectrical impedance spectroscopy method in measurements of selected characteristics of the human organism are discussed as well as plans for future development of dedicated, specialized equipment.W pracy przedstawiono wyniki wstępnych eksperymentów dotyczących pomiarów impedancji bioelektrycznej przy użyciu zintegrowanego skanera AD5933. Zaprezentowano wyniki przeprowadzonych symulacji oraz konstrukcję układu sterowania w oparciu o mikroprocesor. Omówiono możliwe zastosowania tej metody spektroskopii impedancji bioelektrycznej w pomiarach wybranych cech ludzkiego organizmu, a także możliwości przyszłego rozwoju dedykowanego urządzenia specjalistycznego

One-pot method for preparing DNA, RNA, and protein for multiomics analysis

Abstract Typical multiomics studies employ separate methods for DNA, RNA, and protein sample preparation, which is labor intensive, costly, and prone to sampling bias. We describe a method for preparing high-quality, sequencing-ready DNA and RNA, and either intact proteins or mass-spectrometry-ready peptides for whole proteome analysis from a single sample. This method utilizes a reversible protein tagging scheme to covalently link all proteins in a lysate to a bead-based matrix and nucleic acid precipitation and selective solubilization to yield separate pools of protein and nucleic acids. We demonstrate the utility of this method to compare the genomes, transcriptomes, and proteomes of four triple-negative breast cancer cell lines with different degrees of malignancy. These data show the involvement of both RNA and associated proteins, and protein-only dependent pathways that distinguish these cell lines. We also demonstrate the utility of this multiomics workflow for tissue analysis using mouse brain, liver, and lung tissue