HerdChek Salmonella antibody ELISA for the serological monitoring of Salmonella infection in swine

In this paper we first speculated and later on have proved that the increased number of Salmonella positive results, using the Salmonella Tek-Elisa method as compared to the standard technique, was due to false positive results of the former method. We found out that the higher sensitivity of the former method was due to P.stuartii E. cloacae and E.coli bacteria (CRBs), which cross-reacted with the monoclonal antibodies of the method, giving rise to a higher number of Salmonella false positive results. The purpose of the present study was to identify the source of the higher sensitivity of the Elisa Tek technique and propose corrective steps

HerdChek Salmonella antibody ELISA for the serological monitoring of Salmonella infection in swine

In this paper we first speculated and later on have proved that the increased number of Salmonella positive results, using the Salmonella Tek-Elisa method as compared to the standard technique, was due to false positive results of the former method. We found out that the higher sensitivity of the former method was due to P.stuartii E. cloacae and E.coli bacteria (CRBs), which cross-reacted with the monoclonal antibodies of the method, giving rise to a higher number of Salmonella false positive results. The purpose of the present study was to identify the source of the higher sensitivity of the Elisa Tek technique and propose corrective steps.</p

Culturing and Scaling up Stem Cells of Dental Pulp Origin Using Microcarriers

Ectomesenchymal stem cells derived from the dental pulp are of neural crest origin, and as such are promising sources for cell therapy and tissue engineering. For safe upscaling of these cells, microcarrier-based culturing under dynamic conditions is a promising technology. We tested the suitability of two microcarriers, non-porous Cytodex 1 and porous Cytopore 2, for culturing well characterized dental pulp stem cells (DPSCs) using a shake flask system. Human DPSCs were cultured on these microcarriers in 96-well plates, and further expanded in shake flasks for upscaling experiments. Cell viability was measured using the alamarBlue assay, while cell morphology was observed by conventional and two-photon microscopies. Glucose consumption of cells was detected by the glucose oxidase/Clark-electrode method. DPSCs adhered to and grew well on both microcarrier surfaces and were also found in the pores of the Cytopore 2. Cells grown in tissue culture plates (static, non-shaking conditions) yielded 7 × 105 cells/well. In shake flasks, static preincubation promoted cell adhesion to the microcarriers. Under dynamic culture conditions (shaking) 3 × 107 cells were obtained in shake flasks. The DPSCs exhausted their glucose supply from the medium by day seven even with partial batch-feeding. In conclusion, both non-porous and porous microcarriers are suitable for upscaling ectomesenchymal DPSCs under dynamic culture conditions