491 research outputs found
SOS: A simple interactive program for ab initio oligonucleotide sequencing by mass spectrometry
Automated de novo sequencing of nucleic acids by liquid chromatography-tandem mass spectrometry
Electron Capture Dissociation Mass Spectrometry of Tyrosine Nitrated Peptides
In vivo protein nitration is associated with many disease conditions that involve oxidative stress and inflammatory response. The modification involves addition of a nitro group at the position ortho to the phenol group of tyrosine to give 3-nitrotyrosine. To understand the mechanisms and consequences of protein nitration, it is necessary to develop methods for identification of nitrotyrosine-containing proteins and localization of the sites of modification.Here, we have investigated the electron capture dissociation (ECD) and collision-induced association (CID) behavior of 3-nitrotyrosine-containing peptides. The presence of nitration did not affect the CID behavior of the peptides. For the doubly-charged peptides, addition of nitration severely inhibited the production of ECD sequence fragments. However, ECD of the triply-charged nitrated peptides resulted in some singly-charged sequence fragments. ECD of the nitrated peptides is characterized by multiple losses of small neutral species including hydroxyl radicals, water and ammonia. The origin of the neutral losses has been investigated by use of activated ion (AI) ECD. Loss of ammonia appears to be the result of non-covalent interactions between the nitro group and protonated lysine side-chains
Using amino acids for probing structural information of cytochrome c by electrospray ionization mass spectrometry
Reduction of chemical noise in electrospray ionization mass spectrometry by supplemental IR activation
High amplitude short time excitation: A method to form and detect low mass product ions in a quadrupole ion trap mass spectrometer
Selective determination of pyridine alkaloids in tobacco by PFTBA ions/analyte molecule reaction ionization ion trap mass spectrometry
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