Willeria sinensis maserasyon yağı ile Bacillus subtilis ve Candida tropicalis inaktivasyonu

In this study, the antimicrobial effect of maceration oil obtained from W. sinensis flowers oil soaked in olive oil on B. subtilis and C. tropicalis was investigated. In addition, the efficacy of W. sinensis maceration oil on inactivation of the strains of B. subtilis and C. tropicalis inoculated bulgur (pounded wheat) was investigated using dip incubation method. The components of W. sinensis macerate were analyzed by GC-MS and found the main components as olealdehyde (38.03%), oleic acid (29.13%), 9- octadecenoic acid (15.09%), (Z)-9,17-octadecadienal (7.87%) and palmitic acid (5.97%). Broth Microdilution and Agar Well Diffusion Method for antimicrobial activity of W. sinensis and also Modified TDtest for persistent/tolerant levels of microorganisms were used. Minimum Inhibitory Concentrations (MICs) of W. sinensis were 10.3 mg/mL and 9.6 mg/mL for B. subtilis and C. tropicalis while the inhibition zones were 2.23 mm and 2.07 mm, respectively. In TDTest which was made persistent/tolerant screening of microorganisms in W. sinensis condition, both of microrganisms were persistent sensitive. W. sinensis at 50 µL, 100 µL and 150 µL caused an almost 2-log reduction on the number of B. subtilis and C. tropicalis on bulgur.Bu çalışmada, zeytinyağına batırılmış W. sinensis çiçeklerinden elde edilen maserasyon yağının B. subtilis ve C. tropicalis üzerindeki antimikrobiyal etkisi araştırıldı. Ek olarak, W. sinensis maserasyon yağının, bulgura (dövülmüş buğday) aşılanmış B. subtilis ve C. tropicalis suşlarının inaktivasyonu üzerindeki etkinliği daldırma inkübasyon metodu kullanılarak araştırıldı. W. sinensis maserat bileşenleri GC-MS ile analiz edildi ve ana bileşenleri olealdehit (%38.03), oleik asit (%29.13), (Z)-9,17- oktadekadienal asit (%15.09), (Z)-9,17-oktadekadienal (%7.87) ve palmitik asit (%5.97) olarak bulundu. W. sinensis'in antimikrobiyal aktivitesi için Sıvı Mikrodilüsyon ve Agar Kuyucuk Difüzyon Yöntemi ve ayrıca kalıcı/toleranslı mikroorganizma seviyeleri için Modifiye TDtest kullanıldı. W sinensis’in Minimum İnhibitör Konsantrasyonları (MİK), B. subtilis ve C. tropicalis için 10.3 mg/mL ve 9.6 mg/mL iken inhibisyon zonları sırasıyla 2.23 mm ve 2.07 mm idi. W. sinensis varlığında mikroorganizmaların kalıcı/toleranslı taranması yapılan TDTest'te her iki mikroorganizmanın da kalıcı duyarlılığı saptandı. W. sinensis’in 50 µL, 100 µL ve 150 µL’si, bulgur üzerindeki B. subtilis ve C. tropicalis sayısında neredeyse 2 log azalmaya neden oldu

Characterization of the Cytotoxic Compounds of Lactarius salmonicolor R. Heim and Leclair by Gas Chromatography-Mass Spectrometry and Chemometrics

The cytotoxic activities of apolar extract of Lactarius salmonicolor and its fractions and subfractions were screened against colon (CaCo-2), prostate (LNCaP), lung (H1299), and breast (MCF-7) cancerous cell lines. The main extract of Lactarius salmonicolor that was cytotoxic to CaCo-2 (EC50: 137.1 +/- 2.7 mu g/mL) and LNCap (EC50: 131.2 +/- 2.6 mu g/mL) mainly contains fatty acids (99.9%). To characterize the cytotoxic activity, it was fractionated by a silica gel column. Two cytotoxic fractions were re-fractionated to obtain subfractions. The extract, four cytotoxic fractions, and all subfractions were analyzed by gas chromatography-mass spectrometry (GC-MS). The GC-MS data and the cytotoxic activity (EC50) results were classified using principal component analyses (PCA). According to PCA, the cytotoxic fractions containing abundant benzoic acid (0.01%-0.35%), cinnamic acid (0.01%-0.36%), azelaic acid (0.01%-0.92%), 2,4-decadienal (0.01%-0.12%), (Z)-9-palmitoleic acid (0.01%-1.09%), 18-hydroxy 2,4-diene oleic acid (0.01%-26.71%), arachidic acid (0.01%-6.70%), desmosterol (0.01%-12.27%), and steroids (0.01%-9.51%) were clustered. LSP.9-10, which causes another cluster, is caused by 8,11,14- eicosatrienoic acid, 11,14,17-eicosatrienoic acid, 9(Z)-decenoic acid compounds, and saturated fatty acids with 10 and 16 carbons. Hence, these compounds may be responsible for the cytotoxic activity. This study demonstrates that GC-MS and chemometric analysis can identify the components responsible for biological activity in combination with biological activity data.Scientific and Technological Research Council of Turkey [TUBITAK 1001-121Z551]This study is part of D.C .'s Master's thesis and was supported by The Scientific and Technological Research Council of Turkey by project number TUBITAK 1001-121Z551 (Turkiye Bilimsel ve Teknolojik Arastirma Kurumu). DAS:Data will be made available upon request