Building inclusive preschool classrooms: How desirable and feasible is a set of strategies that facilitate teacher-child relationships?

Positive teacher-child relationships promote children’s engagement, as children feel more secure to explore and participate in free or oriented activities. For children with disabilities, a context wherein they can receive the support to maintain a positive engagement in different activities is even more relevant. A scarcity of research exists on how to promote ECEC quality, namely, how to facilitate teacher-child interactions in inclusive environments. This study aims to evaluate preschool teachers’ opinions about the desirability and feasibility of a set of empirically validated strategies to improve teacher-child interactions in ECEC classrooms, for the group and children with disabilities. The participants were 89 Portuguese preschool teachers. Based on a non-systematic literature review, a questionnaire composed of 22 strategies to facilitate teacher-child interactions (in 4 dimensions: emotionally responsive interactions, classroom management, attend to children’s perspectives, and scaffolding learning) was developed. Along with the questionnaire, a set of socio-demographic variables was also collected. ECEC teachers scored significantly higher in the desirability subscale compared with the feasibility subscale in all dimensions and at both the child and the group level. This gap between teachers’ perceived desirability and feasibility provides important insights regarding the dimensions which are important to reinforce in ECEC teachers’ education and professional development. The mean difference between the desirability and feasibility subscales registered a higher effect size at the child’s level than at the group’s level, confirming that the inclusion of children with disabilities in preschool settings remains a challenge. Moreover, the effect size was small to moderate in the Emotionally Responsive Interactions dimension for both child and group levels. These results are aligned with previous studies stating that among different self-identified dimensions for improvement, emotional support is the less evoked by ECEC teachers. Across all dimensions, the main reason teachers give for difficulty in feasibility, both at the group and child’s level, is lack of knowledge. Overall, understanding the reasons teachers attribute to the difference between the strategies’ desirability and feasibility informs the assessment of teacher education needs and might be operationalized as a new observation instrument.info:eu-repo/semantics/publishedVersio

Effect of acetylcholine, pilocarpine, serotonin and adrenaline on the in vitro gastric motility of Hoplias malabaricus (Teleostei)

The in vitro effect of acetylcholine, pilocarpine, serotonina and adrenaline on gastric motility of the teleost Hoplias malabaricus was studied. The stomach of this specieshas spontaneous contractile activity, with no relationship between tension or frequency of contrations and fish weight. Acetylcholine reduced the frequency of contrations at higher concentrations, but had not effect at lower concentrations. The higher doses of this drug increased the tension and tonus of the contrations. Pilocarpine increased the frequency and tension of contractions at higher concentrations, but had not effect at lower concentrations, and increased tonus only at the concentration of 10-7 M. A significant correlation between the doses of acetylcholine and policarpine and tension of gastric contractions was found. Adrenaline and serotonina had not effect on the frequency, tension and tonus of the stomach contractions. The results obtained with acetylcholine and policarpine demonstrate that the stomach of H. malabaricus has muscarinic cholinoceptors. The results obtained with adrenaline and serptonin did not demonstrate the existence of adrenergic or serotonergic receptors in the stomach of this species.o efeito da acetilcolina, pilocarpina, serotonina e adrenalina na motilidade gástrica "in vitro" do teleósteo Hoplias malabaricus foi analisado. O estômago desta espécie apresenta uma atividade contrátil espontânea. Não se detectou nenhuma relação entre tensão ou frequência das contrações estomacais com o peso total dos peixes. A acetilcolina diminuiu a frequência das contrações nas concentrações 5 x 10-8 M e 10-7 M e aumentou a tensão e o tônus das contrações estomacais nestas mesmas concentrações. A pilocarpina aumentou a frequência das contrações estomacais nas concentrações 10-8 M, 5 x 10-8 M e 10-7 M, aumentou a tensão nas concentrações 5 x 10-8 M e 10-7 M e o tônus na concentração 10-7 M. A serotonina e a adrenalina não alteraram a frequência, tensão e tônus das contrações estomacais em nenhuma das concentrações utilizadas. Encontrou-se uma correlação significativa entre as doses de acetilcolina e pilocarpina e a tensão das contrações. Os resultados com acetilcolina e pilocarpina permitem concluir que o estômago de H. malabaricus possui receptores colinérgicos muscarínicos. Os resultados obtidos com adrenalina e serotonina não permitem demonstrar a existência de receptores adrenérgicos e serotoninérgicos no estômago desta espécie

BIM in People2People and Things2People interactive process

In this research work, we present an IoT solution to environment variables using a LoRa transmission technology to give real-time information to users in a Things2People process and achieve savings by promoting behavior changes in a People2People process. These data are stored and later processed to identify patterns and integrate with visualization tools, which allow us to develop an environmental perception while using the system. In this project, we implemented a different approach based on the development of a 3D visualization tool that presents the system collected data, warnings, and other users’ perception in an interactive 3D model of the building. This data representation introduces a new People2People interaction approach to achieve savings in shared spaces like public buildings by combining sensor data with the users’ individual and collective perception. This approach was validated at the ISCTE-IUL University Campus, where this 3D IoT data representation was presented in mobile devices, and from this, influenced user behavior toward meeting campus sustainability goalsinfo:eu-repo/semantics/publishedVersio

Education, well-being, and meaning in life: the case of a senior university in Portugal

A investigação no domínio da educação de adultos tem apontado para o efeito positivo da educação formal com relação ao bem-estar em sentido amplo, no entanto, quanto ao impacto da educação não formal os estudos são ainda escassos, particularmente com populações de idade avançada. Em face de uma população mundial cada vez mais idosa, este estudo, de tipo correlacional ex-post facto, busca investigar de que forma a participação na educação não formal, através de universidades seniores (US) ou da terceira idade, tem potencial transformador no bem-estar subjetivo (BES), autoestima, autoeficácia em saúde e no sentido da vida das pessoas que frequentam este tipo de estabelecimentos educacionais em Portugal. Participaram no estudo 60 seniores, repartidos de igual forma entre usuários de uma US e seniores que não frequentavam uma US ou outro estabelecimento similar. Os dados foram recolhidos mediante o emprego de questionários adequados às variáveis em estudo e de reconhecimento internacional. Os resultados revelam efeitos substanciais no grupo com frequência da US na satisfação com a vida e afetividade negativa, na autoeficácia em saúde e na procura de sentido na vida, em relação ao grupo de controle. Quanto à afetividade positiva, enquanto componente afetiva do BES, bem como a autoestima e a presença de sentido na vida, não foram encontradas diferenças significativas, revelando-se os tamanhos do efeito pequenos. Conclui-se pelo contributo positivo das US sobretudo na dimensão cognitiva do BES, na saúde e em uma das dimensões do sentido da vida, podendo ser consideradas contextos de envelhecimento ativo e saudável e de desenvolvimento pessoal, e salienta-se a necessidade de continuidade das investigações, no sentido de superação das limitações encontradas.Research in the field of adult education has shown the positive impact of formal education on overall well-being. However, research on the impact of non-formal education remains scarce, particularly among older people. Given the phenomenon of an increasingly global ageing population, this ex-post facto correlational study aims to investigate how participation in non-formal education through senior or third age universities has a transformative potential on subjective well-being (SWB), self-esteem, health self-efficacy, and meaning in life for those attending such educational institutions in Portugal. Sixty seniors participated in the study, evenly split between users of a senior university (SU) and seniors who did not attend an SU or similar institution. Data were collected using internationally recognised questionnaires appropriate to the studied variables. The results show substantial effects in the SU attendance group on life satisfaction, negative affect, health self-efficacy, and the search for meaning in life compared to the control group. No significant differences were found for positive affect, as an affective component of SWB, self-esteem, or for the presence of meaning in life, with small effect sizes. The study concludes that senior universities contribute positively, particularly in the cognitive dimension of SWB, to health self-efficacy and to a dimension of meaning in life, supporting their role as contexts for active and healthy ageing and personal development. The need for further research on the topic is highlighted in order to overcome the identified limitations

Dietary parameters in patients with drug allergy: Assessing dietary inflammatory index

Background Research on the increasing incidence of allergic diseases evidenced the role of diet as a potential key factor. Diet can modulate the low-grade systemic inflammation related to obesity and several diseases. There are no published data on drug allergy. Aim To investigate a potential association between diet, including dietary inflammatory index (DII), and drug allergy. Also, to evaluate correlations between diet and obesity, inflammatory and metabolic parameters in patients with drug allergy. Methods Ninety consecutive patients studied for suspected drug allergy were evaluated in terms of dietary parameters, anthropometric measurements, bioimpedance and biochemical analysis. DII was calculated based on information collected from a food frequency questionnaire. Results After diagnostic work-up, 39 patients had confirmed drug allergy and 45 excluded, representing the study group and the control group, respectively. The majority (79%) were female, with mean age of 39.58±13.3 years. The 84 subjects revealed an anti-inflammatory diet pattern. No significative difference was found in DII scores between drug allergic patients and controls (-3.37±0.95 vs -3.39±0.86, p = 0.985). However, the patients with drug allergy revealed higher obesity and inflammatory parameters. A significative negative correlation was found between DII and adiponectin levels, in the control group (r = -0.311, p = 0.040). In the patient group, a significative positive correlation was observed between DII and triglycerides (r = 0.359, p = 0.032). No other correlations were found between DII and the assessed parameters. Patients with drug allergy presented a significative higher intake of mono-unsaturated fatty-acids comparing to controls (19.8±3.7 vs 17.8 ± 4.0, p = 0.021). No other statistically significant differences were achieved in dietary parameters, between patients and controls. Conclusion The population assessed in this study revealed an anti-inflammatory diet profile. Although we have found in a previous work that the same patients with drug allergy revealed higher obesity and inflammatory parameters, the DII did not allow to distinguish between patients with drug allergy or controls. The DII scores correlated with triglycerides levels in the drug allergy patients and inversely with adiponectin levels in the control group. Larger studies are needed to clarify the potential role of the diet in drug allergy and its outcomes. (c) 2022 Dias de Castro et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Perfluoroalkylated substances effects in Xenopus laevis A6 kidney epithelial cells determined by ATR-FTIR spectroscopy and chemometric analysis

The effects of four perfluoroalkylated substances (PFASs), namely, perfluorobutanesulfonate (PFBS), perfluorooctanoic acid (PFOA), perfluorooctanesulfonate (PFOS) and perfluorononanoic acid (PFNA) were assessed in Xenopus laevis A6 kidney epithelial cells by attenuated total reflection Fouriertransform infrared (ATR-FTIR) spectroscopy and chemometric analysis. Principal component analysis-linear discriminant analysis (PCA-LDA) was used to visualize wavenumber-related alterations and ANOVA-simultaneous component analysis (ASCA) allowed data processing considering the underlying experimental design. Both analyses evidenced a higher impact of low-dose PFAS-treatments (10-9 M) on A6 cells forming monolayers, while there was a larger influence of high-dose PFAS-treatments (10-5 M) on A6 cells differentiated into dome structures. The observed dose-response PFAS-induced effects were to some extent related to their cytotoxicity: the EC50-values of most influent PFAS-treatments increased (PFOS<PFNA<PFOA<<PFBS), higherdoses of these chemicals induced a larger impact. Major spectral alterations were mainly attributed to DNA/RNA, secondary protein structure, lipids and fatty acids. Finally, PFOS and PFOA caused a decrease in A6 cell numbers compared to controls, whereas PFBS and PFNA did not significantly change cell population levels. Overall, this work highlights the ability of PFASs to alter A6 cells, whether forming monolayers or differentiated into dome structures, and the potential of PFOS and PFOA to induce cell death

Emerging and innovative techniques for arsenic removal applied to a small water supply system

The impact of arsenic on human health has led its drinking water MCL to be drastically reduced from 50 to 10 ppb. Consequently, arsenic levels in many water supply sources have become critical. This has resulted in technical and operational impacts on many drinking water treatment plants that have required onerous upgrading to meet the new standard. This becomes a very sensitive issue in the context of water scarcity and climate change, given the expected increasing demand on groundwater sources. This work presents a case study that describes the development of low-cost techniques for efficient arsenic control in drinking water. The results obtained at the Manteigas WTP (Portugal) demonstrate the successful implementation of an effective and flexible process of reactive filtration using iron oxide. At real-scale, very high removal efficiencies of over 95% were obtained.(undefined

Spider silk-bone sialoprotein fusion proteins for bone tissue engineering

The remarkable mechanical characteristics of the spider silk protein major ampullate spidroin protein suggest this polymer as a promising biomaterial to consider for the fabrication of scaffolds for bone regeneration. Herein, a new functionalized spider silk-bone sialoprotein fusion protein was designed, cloned, expressed, purified and the osteogenic activity studied. Bone sialoprotein (BSP) is a multidomain protein with the ability to induce cell attachment and differentiation and the deposition of calcium phosphates (CaP). Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) was used to assess the secondary structure of the fusion protein. In vitro mineralization studies demonstrated that this new fusion protein with BSP retained the ability to induce the deposition of CaP. Studies in vitro indicated that human mesenchymal stem cells had significant improvement towards osteogenic outcomes when cultivated in the presence of the new fusion protein vs. silk alone. The present work demonstrates the potential of this new fusion protein for future applications in bone regenerationPhD grant SFRH/BD/28603/2006; Chimera project, PTDC/EBB-EBI/109093/2008; NIH, P41 EB002520, EB003210 and DE017207.Foundation for Science and Technolog

Development of clozapine tablets by direct compression - analysis of pharmaceutical equivalence by dissolution profiles

The aim of this work was to develop clozapine tablets that can be classified as a pharmaceutical equivalent to a reference brand product. Tablets were produced by direct compression and dissolution tests were realized in order to evaluate the dissolution profiles. The results show that the tablets can be classified as immediate release dosage forms due to clozapine fast release, and such release was dependent on the amount of sodium croscarmelose in the formulation. Analysis of f1 and f2 factors was frustrated due to the fast drug release; the tablets were analyzed by the dissolution efficiency and the dissolution curve shape. The dissolution efficiency was higher than 98 % and the analysis of the dissolution shape curve showed that the tablets from one batch of the developed formulations were similar to the reference brand product. The clozapine tablets obtained in this study can be considered as pharmaceutically equivalent to the reference brand product.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Mineralization of chitosan membrane using a double diffusion system for bone related applications

Chitosan membranes were subjected to a pre-treatment in a double diffusion system, with a calcium solution in one chamber and a phosphate solution in the other chamber. Both chambers were separated by the chitosan membrane and subject to three mineralization periods (5, 10 and 15 minutes). After this pre-treatment the bioactivity of the different calcium phosphate coatings formed was tested for different periods of immersion time, 7, 14 and 21 days at room temperature and 37ºC, in acellular simulated body fluid (1.0x). The results obtained demonstrated that the calcium phosphate coatings formed during the pre-treatment process are bioactive. It was found that the calcification is effective just in the side of the membrane exposed to the calcium solution chamber. This enabled to develop membranes with asymmetric osteoinductive properties that can be useful in different orthopedic applications