1,568 research outputs found

    Factor de riego para nectarino

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    Ministerio de Economía y Competitividad AGL2013-49047-C02-2

    Effect of Acipimox on Plasma Lipids and Glucose/Insulin in Pregnant Rats

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    To determine how a reduction in maternal hypertriglyceridemia during late pregnancy may affect glucose/insulin relationships, pregnant and virgin rats were orally treated with acipimox, a potent antilipolytic agent. In 20-day pregnant rats receiving 80 mg of acipimox, plasma triglycerides (TG), free fatty acids (FFA), and glycerol decreased more than in virgin rats shortly after the drug (up to 7 hours), when compared with animals treated with distilled water, whereas plasma glucose level was unaffected by the treatment in either group of rats. When acipimox was given every 12 hours from day 17 to day 20 of pregnancy, plasma TG, FFA, and glycerol levels progressively increased, whereas they either decreased or did not change in virgin rats receiving the same treatment, with no effect in plasma glucose levels in either group. Fetal body weight was lower than in controls in 20-day pregnant rats that received acipimox for 3 days. On day 20 of pregnancy, 3 hours after receiving acipimox or distilled water, rats received a 2 g glucose/kg oral load and it was found that the change in plasma glucose was similar in both groups, whereas the increase in plasma insulin was greater in pregnant rats treated with acipimox. However, no difference was found in either variable after the oral glucose load in virgin rats receiving acipimox or distilled water. No differences in plasma glucose levels were found after intravenous (IV) administration of insulin in pregnant rats treated or not treated with acipimox. In conclusion, present results show that administration of acipimox during the last days of gestation inhibited lipolysis and decreased fetal weight. Over a short period of time, in pregnant rats, reductions of plasma FFA and TG after acipimox treatment improved the glucose-induced insulin release, but did not seem to have any effect in peripheral insulin resistance

    Ontogenetic changes in DNA and RNA content of laboratory-reared Prochilodus lineatus larvae: use of RNA/DNA ratios as indicators of nutritional condition

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    The aims of the present study were to describe the ontogenetic performance of DNA and RNA content, as well as to assess the nutritional condition of Prochilodus lineatus larvae reared under different feeding treatments. The experiment consisted of two parts, the first 6-14 days after hatching (DAH) and the second 15-21 DAH. In the first part of the experiment, larvae were kept under one of two feeding treatments: fed or starved. During the second part of the experiment, larvae were kept under one of four treatments: fed, fed until 14 DAH and later starved, starved until 14 DAH and fed later or starved. It is of note that the size and weight reached of larvae that were starved during early development and later received food were higher than those of than larvae in the other feeding treatments. In addition, instantaneous growth rates were higher and nutritional condition was better in larvae that were initially starved and fed later. The availability of food following a period of starvation seems to trigger larval metabolism and cellular division. Consequently, we recommend maintaining appropriate food provision in the aquaculture of P. lineatus larvae, especially in advanced developmental stages. We verified that RNA/DNA ratio is affected by the feeding condition and that it is sensitive enough to promptly reflect changes in feeding conditions of P. lineatus larvae. Thus, the RNA/DNA ratio could be used to assess nutritional condition of both wild and cultured P. lineatus larvae.Fil: Diaz, Marina Vera. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Gómez, M. I.. Dirección de Pesca Continental; ArgentinaFil: Sánchez, S.. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; ArgentinaFil: Fuentes, Carlos Mariano. Dirección de Pesca Continental; Argentin

    Sobre la oscilabilidad de las soluciones de la ecuación x′′ + f(x, x′, t) = 0

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    En este trabajo se determinan condiciones necesarias o suficientes para que las soluciones de la ecuación de segundo orden no lineal x′′+ f(x, x′, t) = 0 sean oscilantes. Se analiza el caso particular f(x, x′, t) = a(t)f(x)h(x′), se comparan los resultados obtenidos en uno y otro caso y se dan ejemplos que patentizan estos resultados.   In this work we determine necessary and sufficient conditions in order to the solutions of the second-order, nonlinear differential equation x′′+ f(x, x′, t) = 0 be oscillating. We make the analysis of the particular f(x, x′, t) = a(t)f(x)h(x′), comparing both cases and bringing examples to support those results

    Sobre la oscilabilidad de las soluciones de la ecuación x′′ + f(x, x′, t) = 0

    Get PDF
    En este trabajo se determinan condiciones necesarias o suficientes para que las soluciones de la ecuación de segundo orden no lineal x′′+ f(x, x′, t) = 0 sean oscilantes. Se analiza el caso particular f(x, x′, t) = a(t)f(x)h(x′), se comparan los resultados obtenidos en uno y otro caso y se dan ejemplos que patentizan estos resultados. 

    Sobre la oscilabilidad de las soluciones de la ecuación x′′ + f(x, x′, t) = 0

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    En este trabajo se determinan condiciones necesarias o suficientes para que las soluciones de la ecuación de segundo orden no lineal x′′+ f(x, x′, t) = 0 sean oscilantes. Se analiza el caso particular f(x, x′, t) = a(t)f(x)h(x′), se comparan los resultados obtenidos en uno y otro caso y se dan ejemplos que patentizan estos resultados.   In this work we determine necessary and sufficient conditions in order to the solutions of the second-order, nonlinear differential equation x′′+ f(x, x′, t) = 0 be oscillating. We make the analysis of the particular f(x, x′, t) = a(t)f(x)h(x′), comparing both cases and bringing examples to support those results

    Forage Yield and Nutritive Value of 30 Cultivars of Maize for Silage in the Highland Valleys of Central Mexico

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    In Mexico, the selection of maize cultivars for forage has mainly been based on dry matter (DM) yields, not considering nutritional quality as an important evaluation parameter. The objective of this study was to assess forage yield and nutritive value of Highland and Subtropical maize cultivars for silage in the Highland Valleys of Central México

    As cerâmicas cinzentas da Sé de Lisboa

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    As escavações que o IPPAR promoveu no Claustro da Sé de Lisboa permitiram recolher um abundante espólio da Idade do Ferro. Entre esse espólio, de características eminentemente orientalizantes, destacava-se a cerâmica cinzenta que agora entendemos publicar. O estudo que realizámos incidiu, em primeiro lugar, sobre os aspectos estritamente formais, tendo-se definido diversos tipos e variantes. A definição tipológica originou também uma análise pormenorizada desta espécie cerâmica num contexto mais geral, concretamente peninsular, tendo-se tomado em consideração a homogeneidade e diversidade observadas em termos morfológicos, entre as diversas regiões que, durante a Idade do Ferro, produziram e consumiram cerâmicas cinzentas.The excavations that IPPAR undertook in the Cloister of the Sé Cathedral of Lisbon allowed for the recovery of abundant material from the Iron Age. Among this material, of predominantly orientalizing characteristics, stood out the grey ceramics that we now publish. The study that we carried out focused, in the first place, on those strictly formal aspects, having defined diverse types and varieties. The typological definition led also to a detailed analysis of this type of ceramic in a more general context, squarely peninsular, having taken into consideration the homogeneity and diversity observed in morphological terms, between the diverse regions that, during the Iron Age, produced and consumed gray ceramics.info:eu-repo/semantics/publishedVersio

    miRNA Expression Profile Analysis in Kidney of Different Porcine Breeds

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    MicroRNAs (miRNAs) are important post-transcriptional regulators in eukaryotes that target mRNAs repressing their expression. The uncertain process of pig domestication, with different origin focuses, and the selection process that commercial breeds suffered, have generated a wide spectrum of breeds with clear genetic and phenotypic variability. The aim of this work was to define the miRNAs expression profile in kidney of several porcine breeds. Small RNA libraries from kidney were elaborated and high-throughput sequenced with the 454 Genome Sequencer FLX (Roche). Pigs used were classified into three groups: the European origin group (Iberian breed and European Wild Boar ancestor), European commercial breeds (Landrace, Large White and Piétrain breeds) and breeds with Asian origin (Meishan and Vietnamese breeds). A total of 229 miRNAs were described in the pig kidney miRNA profile, including 110 miRNAs out of the 257 previously described pig miRNAs and 119 orthologous miRNAs. The most expressed miRNAs in pig kidney microRNAome were Hsa-miR-200b-3p, Ssc-miR-125b and Ssc-miR-23b. Moreover, 5 novel porcine miRNAs and 3 orthologous miRNAs could be validated through RT-qPCR. miRNA sequence variation was determined in 116 miRNAs, evidencing the presence of isomiRs. 125 miRNAs were differentially expressed between breed groups. The identification of breed-specific miRNAs, which could be potentially associated to certain phenotypes, is becoming a new tool for the study of the genetic variability underlying complex traits and furthermore, it adds a new layer of complexity to the interesting process of pig evolution
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