Structural and Physiological Exploration of Salmonella Typhi YfdX Uncovers Its Dual Function in Bacterial Antibiotic Stress and Virulence

YfdX is a prokaryotic protein encoded by several pathogenic bacteria including Salmonella enterica serovar Typhi, which causes one of the most fatal infectious diseases, typhoid fever. YfdX is a product of the yfdXWUVE operon and is known to be under the control of EvgA, a regulator protein controlling the expression of several proteins involved in response to environmental stress, in Escherichia coli. Nevertheless, unlike other proteins encoded by the same operon, the structural and physiological aspects of YfdX have been poorly characterized. Here, we identified a previously unknown pH-dependent stoichiometric conversion of S. Typhi YfdX between dimeric and tetrameric states; this conversion was further analyzed via determining its structure by X-ray crystallography at high resolution and by small-angle X-ray scattering in a solution state and via structure-based mutant studies. Biologically, YfdX was proven to be critically involved in Salmonella susceptibility to two β-lactam antibiotics, penicillin G and carbenicillin, as bacterial growth significantly impaired by its deficiency upon treatment with each of the two antibiotics was recovered by chromosomal complementation. Furthermore, by using Galleria mellonella larvae as an in vivo model of Salmonella infection, we demonstrated that Salmonella virulence was remarkably enhanced by YfdX deficiency, which was complemented by a transient expression of the wild-type or dimeric mutant but not by that of the monomeric mutant. The present study work provides direct evidence regarding the participation of YfdX in Salmonella antibiotic susceptibility and in the modulation of bacterial virulence, providing a new insight into this pathogen’s strategies for survival and growth

Estimating the number of severe COVID-19 cases and COVID-19-related deaths averted by a nationwide vaccination campaign in Republic of Korea

Objectives The Korea Disease Control and Prevention Agency promotes vaccination by regularly providing information on its benefits for reducing the severity of coronavirus disease 2019 (COVID-19). This study aimed to analyze the number of averted severe COVID-19 cases and COVID-19-related deaths by age group and quantify the impact of Republic of Korea’s nationwide vaccination campaign. Methods We analyzed an integrated database from the beginning of the vaccination campaign on February 26, 2021 to October 15, 2022. We estimated the cumulative number of severe cases and COVID-19-related deaths over time by comparing observed and estimated cases among unvaccinated and vaccinated groups using statistical modeling. We compared daily age-adjusted rates of severe cases and deaths in the unvaccinated group to those in the vaccinated group and calculated the susceptible population and proportion of vaccinated people by age. Results There were 23,793 severe cases and 25,441 deaths related to COVID-19. We estimated that 119,579 (95% confidence interval [CI], 118,901–120,257) severe COVID-19 cases and 137,636 (95% CI, 136,909–138,363) COVID-19-related deaths would have occurred if vaccination had not been performed. Therefore, 95,786 (95% CI, 94,659–96,913) severe cases and 112,195 (95% CI, 110,870–113,520) deaths were prevented as a result of the vaccination campaign. Conclusion We found that, if the nationwide COVID-19 vaccination campaign had not been implemented, the number of severe cases and deaths would have been at least 4 times higher. These findings suggest that Republic of Korea’s nationwide vaccination campaign reduced the number of severe cases and COVID-19 deaths

Study of the decays B->D_s1(2536)+ anti-D(*)

We report a study of the decays B -> D_s1(2536)+ anti-D(*), where anti-D(*) is anti-D0, D- or D*-, using a sample of 657 x 10^6 B anti-B pairs collected at the Upsilon(4S) resonance with the Belle detector at the KEKB asymmetric-energy e+e- collider. The branching fractions of the decays B+ -> D_s1(2536)+ anti-D0, B0 -> D_s1(2536)+ D- and B0 -> D_s1(2536)+ D*- multiplied by that of D_s1(2536)+ -> (D*0K+ + D*+K0) are found to be (3.97+-0.85+-0.56) x 10^-4, (2.75+-0.62+-0.36) x 10^-4 and (5.01+-1.21+-0.70) x 10^-4, respectively.Comment: 6 pages, 2 figues, submitted to PRD (RC

First Measurement of Inclusive B -> X_s eta Decays

We report a first measurement of inclusive B -> X_s eta decays, where X_s is a charmless state with unit strangeness. The measurement is based on a pseudo-inclusive reconstruction technique and uses a sample of 657 x 10^6 BB-bar pairs accumulated with the Belle detector at the KEKB e^+e^- collider. For M_{X_s} < 2.6 GeV/c^2, we measure a branching fraction of (26.1 +/- 3.0 (stat) +1.9 -2.1 (syst) +4.0 -7.1 (model)) x 10^-5 and a direct CP asymmetry of A_{CP} = -0.13 +/- 0.04 +0.02 -0.03. Over half of the signal occurs in the range M_{X_s} > 1.8 GeV/c^2.Comment: 6 pages, 1 figure, 1 table, submitted to Physical Review Letter

Search for CP violating charge asymmetry in B^+ -> J/psi K^+ decays

We present the result of a search for charge asymmetry in B^+ -> J/psi K^+ decays. The CP-violating charge asymmetry is measured to be A_CP(B^+ -> J/psi K^+) = [-0.76 +/- 0.50 (stat) +/- 0.22 (syst)]%.Comment: 6 pages, 6 figures, 2 table

Study of B^{+-} -> K^{+-}(K_S K pi)^0 Decay and Determination of eta_c and eta_c(2S) Parameters

We report the results of a study of $B^{\pm}\to K^{\pm}\eta_c$ and $B^{\pm}\to K^{\pm}\eta_c(2S)$ decays followed by $\eta_c$ and $\eta_c(2S)$ decays to $(K_SK\pi)^0$. The results are obtained from a data sample containing 535 million $B\bar{B}$-meson pairs collected by the Belle experiment at the KEKB $e^+e^-$ collider. We measure the products of the branching fractions ${\mathcal B}(B^{\pm}\to K^{\pm}\eta_c){\mathcal B}(\eta_c\to K_S K^{\pm}\pi^{\mp})=(26.7\pm 1.4(stat)^{+2.9}_{-2.6}(syst)\pm 4.9(model))\times 10^{-6}$ and ${\mathcal B}(B^{\pm}\to K^{\pm}\eta_c(2S)){\mathcal B}(\eta_c(2S)\to K_S K^{\pm}\pi^{\mp})=(3.4^{+2.2}_{-1.5}(stat+model)^{+0.5}_{-0.4} syst))\times 10^{-6}$. Interference with the non-resonant component leads to significant model uncertainty in the measurement of these product branching fractions. Our analysis accounts for this interference and allows the model uncertainty to be reduced. We also obtain the following charmonia masses and widths: $M(\eta_c)=(2985.4\pm 1.5(stat)^{+0.5}_{-2.0}(syst))$ MeV/$c^2$, $\Gamma(\eta_c)=(35.1\pm 3.1(stat)^{+1.0}_{-1.6}(syst))$ MeV/$c^2$, $M(\eta_c(2S))=(3636.1^{+3.9}_{-4.2}(stat+model)^{+0.7}_{-2.0}(syst))$ MeV/$c^2$, $\Gamma(\eta_c(2S))=(6.6^{+8.4}_{-5.1}(stat+model)^{+2.6}_{-0.9}(syst))$ MeV/$c^2$.Comment: 23 pages, 10 figures, submitted to PL

Search for Lepton-number-violating B+->D-l+l'+ Decays

We perform the first search for lepton-number-violating B+->D-l+l'+ decays, where l and l' stand for e or mu, using 772 x 10^6 BB-bar pairs accumulated at the Upsilon(4S) resonance with the Belle detector at the KEKB e^+e^- collider. No evidence for these decays has been found. Assuming uniform three-body phase space distributions for the D-l+l+ decays, we set the following upper limits on the branching fractions at 90% confidence level: Br(B+ -> D-e+e+) < 2.6x10^-6, Br(B+ -> D-e+mu+) D-mu+mu+) < 1.0x10^-6.Comment: 7 pages, 2 figure

The Physics of the B Factories

This work is on the Physics of the B Factories. Part A of this book contains a brief description of the SLAC and KEK B Factories as well as their detectors, BaBar and Belle, and data taking related issues. Part B discusses tools and methods used by the experiments in order to obtain results. The results themselves can be found in Part C