16 research outputs found
Phase II study of capecitabine and trastuzumab combination chemotherapy in patients with HER2 overexpressing metastatic breast cancers resistant to both anthracyclines and taxanes
Shaft Furnace Simulation by Mathematical Model Considering Coke Gasification Rate in High Temperature
Shaft Furnace Simulation by Mathematical Model Considering Coke Gasification Rate in High Temperature
Tug-of-war between actomyosin-driven antagonistic forces determines the positioning symmetry in cell-sized confinement
細胞内構造の配置対称性が決まる仕組みを解明 --人工細胞と物理学からメカニズムに迫る--. 京都大学プレスリリース. 2020-06-15.The tug-of-war at the heart of cellular symmetry. 京都大学プレスリリース. 2020-06-26.Symmetric or asymmetric positioning of intracellular structures including the nucleus and mitotic spindle steers various biological processes such as cell migration, division, and embryogenesis. In typical animal cells, both a sparse actomyosin meshwork in the cytoplasm and a dense actomyosin cortex underneath the cell membrane participate in the intracellular positioning. However, it remains unclear how these coexisting actomyosin structures regulate the positioning symmetry. To reveal the potential mechanism, we construct an in vitro model composed of cytoplasmic extracts and nucleus-like clusters confined in droplets. Here we find that periodic centripetal actomyosin waves contract from the droplet boundary push clusters to the center in large droplets, while network percolation of bulk actomyosin pulls clusters to the edge in small droplets. An active gel model quantitatively reproduces molecular perturbation experiments, which reveals that the tug-of-war between two distinct actomyosin networks with different maturation time-scales determines the positioning symmetry