Measurements of time dependent CP asymmetry in B→VVB \to VV decays with BELLE

A study of CP violation in $B\to J/\psi K^*(K_S^0\pi^0)$ decays by time dependent angular analysis is discussed. Status of time independent analyses for other $B\to VV$ decays is also reported. The data used for the analyses are taken with the Belle detector at KEK.Comment: 4 pages, 3 figures, Proceeding of the talk in parallel session (CP-3-5) at ICHEP2002, Amsterdam, Netherland, 24-31 July (2002

Quantum corrections to the spin-independent cross section of the inert doublet dark matter

The inert Higgs doublet model contains a stable neutral boson as a candidate of dark matter. We calculate cross section for spin-independent scattering of the dark matter on nucleon. We take into account electroweak and scalar quartic interactions, and evaluate effects of scattering with quarks at one-loop level and with gluon at two-loop level. These contributions give an important effect for the dark matter mass to be around m_h/2, because a coupling with the standard model Higgs boson which gives the leading order contribution should be suppressed to reproduce the correct amount of the thermal relic abundance in this mass region. In particular, we show that the dark matter self coupling changes the value of the spin-independent cross section significantly.Comment: 38 pages, 18 figure

Muon specific two-Higgs-doublet model

We investigate a new type of a two-Higgs-doublet model as a solution of the muon $g-2$ anomaly. We impose a softly-broken $Z_4$ symmetry to forbid tree level flavor changing neutral currents in a natural way. This $Z_4$ symmetry restricts the structure of Yukawa couplings. As a result, extra Higgs boson couplings to muons are enhanced by a factor of $\tan\beta$, while their couplings to all the other standard model fermions are suppressed by $\cot\beta$. Thanks to this coupling property, we can avoid the constraint from leptonic $\tau$ decays in contrast to the lepton specific two-Higgs-doublet model, which can explain the muon $g-2$ within the 2$\sigma$ level but cannot within the $1\sigma$ level due to this constraint. We find that the model can explain the muon $g-2$ within the 1$\sigma$ level satisfying constraints from perturbative unitarity, vacuum stability, electroweak precision measurements, and current LHC data.Comment: 24 pages, 3 figure

Proliferation of Luteal Steroidogenic Cells in Cattle

The rapid growth of the corpus luteum (CL) after ovulation is believed to be mainly due to an increase in the size of luteal cells (hypertrophy) rather than an increase in their number. However, the relationship between luteal growth and the proliferation of luteal steroidogenic cells (LSCs) is not fully understood. One goal of the present study was to determine whether LSCs proliferate during CL growth. A second goal was to determine whether luteinizing hormone (LH), which is known have roles in the proliferation and differentiation of follicular cells, also affects the proliferation of LSCs. Ki-67 (a cell proliferation marker) was expressed during the early, developing and mid luteal stages and some Ki-67-positive cells co-expressed HSD3B (a steroidogenic marker). DNA content in LSCs isolated from the developing CL increased much more rapidly (indicating rapid growth) than did DNA content in LSCs isolated from the mid CL. The cell cycle-progressive genes CCND2 (cyclin D2) and CCNE1 (cyclin E1) mRNA were expressed more strongly in the small luteal cells than in the large luteal cells. LH decreased the rate of increase of DNA in LSCs isolated from the mid luteal stage but not in LSCs from the developing stage. LH suppressed CCND2 expression in LSCs from the mid luteal stage but not from the developing luteal stage. Furthermore, LH receptor (LHCGR) mRNA expression was higher at the mid luteal stage than at the developing luteal stage. The overall results suggest that the growth of the bovine CL is due to not only hypertrophy of LSCs but also an increase in their number, and that the proliferative ability of luteal steroidogenic cells decreases between the developing and mid luteal stages

Multiplex Genome Editing in Tomato

Several expression systems for multiple guide RNA (gRNAs) have been developed in the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9) system to induce multiple-gene modifications in plants. Here, we evaluated mutation efficiencies in the tomato genome using multiplex CRISPR/Cas9 vectors consisting of various Cas9 expression promoters with multiple gRNA expression combinations. In transgenic tomato calli induced with these vectors, mutation patterns varied depending on the promoters used to express Cas9. By using the tomato ELONGATION FACTOR-1α (SlEF1α) promoter to drive Cas9, occurrence of various types of mutations with high efficiency was detected in the tomato genome. Furthermore, sequence analysis showed that the majority of mutations using the multiplex system with the SlEF1α promoter corresponded to specific mutation pattern of deletions produced by self-ligation at two target sites of CRISPR/Cas9 with low mosaic mutations. These results suggest that optimizing the Cas9 expression promoter used in CRISPR/Cas9-mediated mutation improves multiplex genome editing, and could be used effectively to disrupt functional domains precisely in the tomato genome

都市交通市場の発展過程の動学分析ならびに開発途上国における交通投資戦略

学位の種別: 課程博士審査委員会委員 : （主査）東京大学教授 加藤 浩徳, 東京大学教授 堀井 秀之, 東京大学教授 羽藤 英二, 東京大学特任教授 西沢 利郎, 首都大学東京准教授 石倉 智樹University of Tokyo(東京大学

SlIAA9 Controls Tomato Elongation

Tomato INDOLE-3-ACETIC ACID9 (SlIAA9) is a transcriptional repressor in auxin signal transduction, and SlIAA9 knockout tomato plants develop parthenocarpic fruits without fertilization. We generated sliaa9 mutants with parthenocarpy in several commercial tomato cultivars (Moneymaker, Rio Grande, and Ailsa Craig) using CRISPR-Cas9, and null-segregant lines in the T1 generation were isolated by self-pollination, which was confirmed by PCR and Southern blot analysis. We then estimated shoot growth phenotypes of the mutant plants under different light (low and normal) conditions. The shoot length of sliaa9 plants in Moneymaker and Rio Grande was smaller than those of wild-type cultivars in low light conditions, whereas there was not clear difference between the mutant of Ailsa Craig and the wild-type under both light conditions. Furthermore, young seedlings in Rio Grande exhibited shade avoidance response in hypocotyl growth, in which the hypocotyl lengths were increased in low light conditions, and sliaa9 mutant seedlings of Ailsa Craig exhibited enhanced responses in this phenotype. Fruit production and growth rates were similar among the sliaa9 mutant tomato cultivars. These results suggest that control mechanisms involved in the interaction of AUX/IAA9 and lights condition in elongation growth differ among commercial tomato cultivars