Получение и свойства микрочастиц поли(3-гидроксибутирата-со‑3- гидроксивалерата), нагруженных рифампицином

In this work, polyhydroxyalkanoates-based microparticles containing antibacterial drug rifampicin were obtained. The main characteristics of the microparticles were investigated: the particles yield, encapsulation efficiency and drug loading, average radius, surface morphology, polydispersity index, zeta potential and cytotoxicity towards HeLa cells. Loading of RIF into microparticles leads to the substantial decrease of its cytotoxicityВ настоящей р аботе о писан п роцесс п олучения м икрочастиц п оли(3-гидроксибутирата-со‑3- гидроксивалерата), содержащих антибактериальный препарат рифампицин. Определены основные параметры процесса получения и свойства микрочастиц: выход, эффективность инкапсулирования и включение препарата, средние размеры, морфология поверхности, индексы полидисперсности, дзета-потенциал, цитотоксичность в модельной культуре клеток HeLa. Установлено, что цитотоксичность полученной системы существенно ниже таковой для чистого РИ

Influence of PHA Substrate Surface Characteristics on the Functional State of Endothelial Cells

The needs of modern regenerative medicine for biodegradable polymers are wide and varied. Restoration of the viability of the vascular tree is one of the most important components of the preservation of the usefulness of organs and tissues. The creation of vascular implants compatible with blood is an important task of vascular bioengineering. The function of the endothelial layer of the vessel, being largely responsible for the development of thrombotic complications, is of great importance for hemocompatibility. The development of surfaces with specific characteristics of biomaterials that are used in vascular technologies is one of the solutions for their correct endothelialization. Linear polyhydroxyalkanoates (PHAs) are biodegradable structural polymeric materials suitable for obtaining various types of implants and tissue engineering, having a wide range of structural and physicomechanical properties. The use of PHA of various monomeric compositions in endothelial cultivation makes it possible to evaluate the influence of material properties, especially surface characteristics, on the functional state of cells. It has been established that PHA samples with the inclusion of 3-hydroxyhexanoate have optimal characteristics for the formation of a human umbilical vein endothelial cell, HUVEC, monolayer in terms of cell morphology as well as the levels of expression of vinculin and VE-cadherin. The obtained results provide a rationale for the use of PHA copolymers as materials for direct contact with the endothelium in vascular implants