Possibilities in the application of solid lipid nanoparticles in combination with 5-fluorouracil to overcome the drugresistance of non-small cell lung cancer cell line A549

Introduction: Multidrug resistance of non-small cell lung cancer cells is associated with a high percentageof therapeutic failures. The aim of this study was to assess the ability of solid lipid nanoparticles as atransporter of the conventionally used cytostatic (5-fluorouracil) to overcome the resistance of A549 cells.Material and methods: MTT assay was used to assess the differences in viability of cells treated with5-fluorouracil alone or in combination with different types of solid lipid nanoparticles. Type of cell deathand distribution of cell cycle phases were evaluated using flow cytometry.Results: The use of nanoparticles as a 5-fluorouracil transporter reduced the viability of A549 cells to agreater extent than the cytostatic alone. This was mainly due to the increase in apoptosis, but also necrosisand cell cycle arrest.Conclusion: Our results indicate the great potential of nanotechnology in the treatment of non-small celllung cancer. By using nanoparticles, it is possible to sensitise tumour cells to cytostatics to which theyare normally resistant. In addition, literature data confirm the safety of solid lipid nanoparticle application

The influence of house dust extract on normal lung cell Mrc5 and non-small lung carcinoma A549

Introduction. Desensitisation is a therapeutic method of allergic disease treatment, but its impact on the cellular level remains to be elucidated. The purpose of this study was to identify the influence of house dust extract on two cell lines types: non-small lung cancer A549 and non-cancerous lung fibroblast Mrc5. Furthermore, we analysed cell viability, type of cell death, and reorganisation of mainly cytoskeletal proteins such as vimentin, F-actin, and b-tubulin. Material and methods. To determine the cell viability, the MTT test was used. The type of cell death was analysed using double staining of annexin V and iodide propidium. The reorganisation of cytoskeletal proteins was evaluated by fluorescent staining and microscopy observation. Results. Our data presented non-statistical differences in a population of live, apoptotic, and necrotic cells. We did not observe significant abnormalities in cytoskeletal reorganisation. Moreover, Mrc5 cell line exhibited a lower sensitivity for house dust extract in comparison to A549 cell line. Conclusions. Our study suggests that desensitisation has no significant influence on survival and cytoskeleton in both cell lines, which correlate with potential use of this method in cancer patients. Obviously, the choice of these kinds of treatment should be used carefully in consultation with a specialist. Additionally, to our knowledge, it was the first presentation of the influence of house dust extract on cells in the context of the main cytoskeletal reorganisations.

Influence of dexamethasone and doxorubicin on inhibition of hypoxia- -induced metastatic potential in HepG2 cell line

One of the commonly applied methods in the case of median to advance stages of liver cancer is the transarterial chemoembolization (TACE) procedure. It involves the administration of relatively high doses of cytostatics to the tumour-supplying artery followed by the embolization of the vessel. It limits the drug action almost only to the tumour mass. However, this also reduces the availability of oxygen, which stimulates cell migration. Therefore, the study aimed to assess how the introduction of an additional drug — dexamethasone and its combination with doxorubicin will impact the viability and migration of HepG2 cells under hypoxia-mimic conditions. To assess the basic response of the cells to the drugs and evaluate the interaction between them MTT assay and apoptosis assay were used. To analyse the migratory potential transwell migration assay was applied. Epithelial-mesenchymal transition (EMT) markers and apoptosis-related proteins were studied using Western blot assay. Hypoxia-mimic conditions were induced using pretreatment with cobalt chloride. The obtained results suggest that the developed doxorubicin: dexamethasone combination limits hypoxia-induced increase in the migratory potential of HCC cells, which is connected with the inhibition of the EMT process and directing cells to death on the cellular level