Verification and Optimization of a PLC Control Schedule

We report on the use of the SPIN model checker for both the verification of a process control program and the derivation of optimal control schedules. This work was carried out as part of a case study for the EC VHS project (Verification of Hybrid Systems), in which the program for a Programmable Logic Controller (PLC) of an experimental chemical plant had to be designed and verified. The intention of our approach was to see how much could be achieved here using the standard model checking environment of SPIN/Promela. As the symbolic calculations of real-time model checkers can be quite expensive it is interesting to try and exploit the efficiency of established non-real-time model checkers like SPIN in those cases where promising work-arounds seem to exist. In our case we handled the relevant real-time properties of the PLC controller using a time-abstraction technique; for the scheduling we implemented in Promela a so-called variable time advance procedure. For this case study these techniques proved sufficient to verify the design of the controller and derive (time-)optimal schedules with reasonable time and space requirements

HCV-specific T-cell responses in injecting drug users: evidence for previous exposure to HCV and a role for CD4+ T cells focussing on nonstructural proteins in viral clearance.

In order to understand the parameters associated with resolved hepatitis C virus (HCV)-infection, we analysed the HCV-specific T-cell responses longitudinally in 13 injecting drug-users (IDUs) with a prospectively identified acute HCV infection. Seven IDUs cleared HCV and six IDUs remained chronically infected. T-cell responses were followed in the period needed to resolve and a comparable time span in chronic carriers. Ex vivo T-cell responses were measured using interferon-gamma Elispot assays after stimulation with overlapping peptide pools spanning the complete HCV genome. CD4+ memory-T-cell responses were determined after 12-day stimulation with HCV proteins. The maximum response was compared between individuals. The T-cell responses measured directly ex vivo were weak but significantly higher in resolvers compared to chronic carriers, whereas the CD4+ memory-T-cell response was not different between resolvers and chronic carriers. However, HCV Core protein was targeted more often in chronic carriers compared to individuals resolving HCV infection. CD4+ T-cell responses predominantly targeting nonstructural proteins were associated with resolved HCV infection. Interestingly, observation of memory-T-cell responses present before the documented HCV-seroconversion suggests that reinfections in IDUs occur often. The presence of these responses however, were not predictive for the outcome of infection. However, a transition of the HCV-specific CD4+ memory-T-cell response from targeting Core to targeting nonstructural proteins during onset of infection was associated with a favourable outcome. Therefore, the specificity of the CD4+ memory-T-cell responses measured after 12-day expansion seems most predictive of resolved infectio

Increase in HCV incidence among men who have sex with men in Amsterdam most likely caused by sexual transmission

We retrospectively screened 1836 men who have sex with men (MSM) participating in the Amsterdam Cohort Studies (1984-2003) for hepatitis C virus (HCV) antibodies. HCV incidence was 0.18/100 person-years (PY) in human immunodeficiency virus (HIV)-positive MSM (8/4408 PY [95% confidence interval {CI}, 0.08-0.36]) but was 0/100 PY in MSM without HIV (0/7807 PY [95% CI, 0.00-0.05]). After 2000, HCV incidence among HIV-positive men increased 10-fold to 0.87/100 PY (5/572 PY [95% CI, 0.28-2.03]). Additional hospital cases (n=34) showed that MSM in Amsterdam who acquired HCV infection after 2000 reported high rates of ulcerative sexually transmitted infections (59%) and rough sexual techniques (56%), denied injection drug use, and were infected mainly with the difficult-to-treat HCV genotypes 1 (56%) and 4 (36%). Phylogenetic analysis showed 3 monophyletic clusters of MSM-specific HCV strains. The emergence of an MSM-specific transmission network suggests that HIV-positive MSM with high-risk sexual behaviors are at risk for sexually acquired HCV. Targeted prevention and routine HCV screening among HIV-positive MSM is needed to deter the spread of HC

Antichains: alternative algorithms for LTL satisfiability and model-checking

The linear temporal logic (LTL) was introduced by Pnueli as a logic to express properties over the computations of reactive systems. Since this seminal work, there have been a large number of papers that have studied deductive systems and algorithmic methods to reason about the correctness of reactive programs with regard to LTL properties. In this paper, we propose new efficient algorithms for LTL satisfiability and model-checking. Our algorithms do not construct nondeterministic automata from LTL formulas but work directly with alternating automata using efficient exploration techniques based on antichains. © 2008 Springer-Verlag Berlin Heidelberg.SCOPUS: cp.kinfo:eu-repo/semantics/published14th International Conference onTools and Algorithms for the Construction and Analysis of Systems, TACAS2008"; Budapest; Hungary; 29 March 2008 through 6 April 2008