(S)-3-Acetyl-3-[(R)-1-(4-bromo­phen­yl)-2-nitro­eth­yl]oxolan-2-one

The title compound, C14H14BrNO5, has two chiral C atoms. The quaternary C atom in the oxolanone ring has an S configuration, while the adjacent tertiary C atom has an R configuration. The oxolanone ring adopts an envelope conformation, with the flap C atom lying 0.298 (3) Å from the mean plane of the remaining four atoms. In the crystal, mol­ecules are connected into chains along [010] via weak C—H⋯O hydrogen bonds

Self-organization phenomena of the nonequilibrium process in magmatism of the Fildes Peninsula, West Antarctica

Volcanic geological, petrological and geochemical characteristics of the Tertiary volcanic rocks from the Fildes Peninsula, West Antarctica show that magma evolution was a process of dissipation of heat energy exchanged energy and mass occurring between the magma system and its surrounding environments, and with the feature of dynamic equilibrium and periodicity (stage). In the study volcanic rocks of different types commonly exhibit a multi-grade composite texture and the derivative magmas produced by differentiation of parent magmas in the magma chamber show a zonal structure in the high-level-magma chamber which represent self-organization phenomenon of the nonequilibrium process in magmatism. The self-organization phenomenon is dissipative structure formed under given conditions

Study on the Optimization of Proportion of Fly Ash-Based Solid Waste Filling Material with Low Cost and High Reliability

In order to solve the problem of the high cost of coal-based solid waste bulk stacking and paste filling in the large-scale coal electrification base in East NingXia, in this study, fly ash is skillfully used to replace the broken coal gangue as the mixed filling material. As using a jaw crusher for crushing large coal gangue is expensive, and its energy consumption is relatively high, paste filler using fly ash as aggregate is studied through micro and macro test analyses. Using response surface methodology design software, 29 groups of mix proportion schemes are designed to obtain the best mix proportion. In addition, the radar results of slump, slump flow, and comprehensive strength are obtained by the normalization method. According to the radar chart results of the three normalized indexes, the optimal ratio parameters are as follows: the fly ash in solid phase is 79%, the mass of fly ash to the mass of cement (FA/C) is 6:1, the solid mass concentration is 78%, the fly ash to gasification slag is 1:1, and the results show that σ3d = 2.20 MPa, slump = 205 mm, and flow = 199 mm. Taking the solid mass concentration, FA/C, the fly ash content in solid phase, and the coal gangue-to-gasification slag ratio as independent variables, the influence of single-factor and multi-factor interactions of the independent variables are analyzed based on the response surface model. It is found that the solid mass concentration and FA/C have a very significant effect on the early strength. Replacing coal gangue base with fly ash base can effectively reduce the crushing cost and energy consumption and provide low-cost and highly reliable technical reserves for large-scale filling

Quantitative evaluation of in -situ bioremediation of compound pollution of oil and heavy metal in sediments from the Bohai Sea, China

Owing to the semi-enclosed environment of the Bohai Sea, the ecological effects caused by an oil spill would be significant. A typical in- situ bioremediation engineering project for of oil-spilled marine sediments was performed in the Bohai Sea and a quantitative assessment of the ecological restoration was performed. The bioremediation efficiencies of n-alkane and PAHs in the sediment are 32.84 +/- 21.66% and 50.42 +/- 17.49% after 70 days of bioremediation, and 60.99 +/- 10.14% and 68.01 +/- 18.60% after 210 days, respectively. After 210 days of bioremediation, the degradation rates of two- to three ring PAHs and four-ring PAHs are 84.44 +/- 23.03% and 26.62 +/- 43.76%, respectively. In addition, the concentrations of the heavy metals first increased by 6.00% due to oil spill degradation and release, and then decreased by 72.60% with the degradation of oil caused by bioremediation or vertical migration. According to the continuous tracking monitoring, the composition of the microbial community in the restored area was similar to that in the control area and the clean area in Bohai Sea after 210 days of bioremediation. These results may provide some theoretical and scientific data to understand the degradation mechanism and assessing the ecological remediation efficiency for oil spills in open sea areas

Effects of 10 Hz Repetitive Transcranial Magnetic Stimulation of the Left Dorsolateral Prefrontal Cortex in Disorders of Consciousness

BackgroundWhile repetitive transcranial magnetic stimulation (rTMS) has been applied in treatment of patients with disorders of consciousness (DOC), a standardized stimulation protocol has not been proposed, and its therapeutic effects are inconsistently documented.ObjectivesTo assess the efficacy of rTMS in improving consciousness in patients with persistent minimally conscious state (MCS) or unresponsive wakefulness syndrome (UWS), previously known as vegetative state (VS).MethodA prospective single-blinded study, with selected subjects, was carried out. In total, 16 patients (5 MCS and 11 VS/UWS) with chronic DOC were included. All patients received active 10 Hz rTMS at the left dorsolateral prefrontal cortex (DLPFC), at one session per day, for 20 consecutive days. A single daily session of stimulation consisted of 1,000 pulses (10 s of 10 Hz trains; repeated 10 times with an inter-train interval of 60 s; and 11 min and 40 s for total session). The main outcome measures were changes in the total score on the JFK Coma Recovery Scale-Revised (CRS-R) scale. Additional measures were the impressions of caregivers after the conclusion of the interventions, which were assessed using the Clinical Global Impression-Improvement (CGI-I) scale.ResultsThe CRS-R scores were increased in all 5 MCS patients and 4 of 11 VS/UWS patients, while a significant enhancement of CRS-R scores was observed compared to the baseline in all participants (p = 0.007). However, the improvement was more notable in MCS patients (p = 0.042) than their VS/UWS counterparts (p = 0.066). Based on the CGI-I scores, two patients improved considerably, two improved, six minimally improved, six experienced no change, and none deteriorated. Good concordance was seen between the CGI-I result and the increases in CRS-R scores.ConclusionTreatment of 10 Hz multisession rTMS applied to the left DLPFC is promising for the rehabilitation of DOC patients, especially those in MCS. Further validation with a cohort of a larger sample size is required

Uncover DNA damage and repair-related gene signature and risk score model for glioma

AbstractBackground Glioma is a common primary central nervous system tumor with complex pathogenesis. DNA damage and repair (DDR) is widely involved in regulating cell proliferation and tumorigenesis by correcting and repairing DNA damage mechanisms. Recent studies have reported the following properties in cancer cells in glioma, increased DNA damage and reduced DNA repair capacity. However, the relationship between glioma and DDR-related genes was unclear.Methods DDR-related risk score model was built. The validity of this model was validated in detail through the Kaplan-Meier survival analysis, tumor mutational burden (TMB) analysis, immune cell infiltration, sensitivity to treatment regimens. Moreover, the model’s adaptability was validated in different glioma data cohorts and different glioma subgroups. To further investigate the molecular mechanism of one of DDR-related gene (NUDT1) in glioma, U251 cell was used for the knockdown experiment, followed by MTT, wound healing and transwell analysis.Results Ten prognostic-related DDR-related signature genes were obtained, including EID3, MGMT, YWHAG, PMS1, SHPRH, HUS1, NUDT1, GADD45G, APEX1 and FAM175A. The RT-qPCR results suggested that the latter five genes were highly expressed in glioma patients. Interestingly, high TMB score had longer survival. In high-risk score groups, reduced immune cell infiltration in the tumor microenvironment lead to poorer patient outcomes. Sensitivity to treatment regimens analysis indicated that low-risk score groups were more sensitive to chemotherapeutics. Moreover, the risk score model had a good prediction effect on different glioma datasets and different glioma subgroups. In vitro mechanism study showed that knockdown of NUDT1 reduced tumorigenesis. Furthermore, knockdown of NUDT1 remarkably reduced the expression level of HIF-1α.Conclusion DDR-related risk score model built-in this work has good predictive performance for glioma.Key messagesTen prognostic-related DDR-related signature genes were obtained, including EID3, MGMT, YWHAG, PMS1, SHPRH, HUS1, NUDT1, GADD45G, APEX1 and FAM175A.In high-risk score groups, reduced immune cell infiltration in the tumor microenvironment leads to poorer patient outcomes.The risk score model had a good prediction effect on different glioma datasets and different glioma subgroups.Knockdown of NUDT1 reduced tumorigenesis of glioma and remarkably reduced the expression level of HIF-1α