Assessing the intralobular ducts in mouse mandibular gland

In order to quantify the types of intralobular ducts, we used five adult male mice, sacrificed by cervical dislocation, after exposure to anesthetic. After euthanasing the animals, the mandibular glands were histologically processed. We sectioned the tissue at 5 µm thickness and stained the slides using Goldner’s trichrome staining procedure. We captured images on four different microscopic fields for each animal, subsequently counting each type of intralobulary duct. The granular ducts were the most numerous, followed by the intermediary ones and striated ducts with the lowest number

Observations regarding the structure of the parotid and mandibular glands in rabbit

Our study aims to investigate the histological structure of two major salivary glands in adult rabbit. We used 5 adult rabbits, 3 males and 2 females, from a private breeder in Cluj. We harvested the salivary glands, fixed them in 10% buffered formalin, dehydrated in ethanol (increasing concentration), clarified in n-butanol and embedded in paraffin. The sections (5 µm thickness) were stained with Goldner’s trichrome method and examined with an Olympus BX41 light microscope. Histologically, parotid gland in rabbit contains only one type of acini, which presents morphological characteristics of serous acini. In mandibular gland, the acini are all the same, similar to the ones in parotid gland. Concerning the general aspect and dimension they are much larger and polymorphic

Induced sepsis by cecal ligation and puncture in a rat model: hepatic and renal histological features

Animal models have been developed in an attempt to test potential therapeutic agents. The study aims to determine the hepatic and renal histological features induced by sepsis following cecal ligation and puncture (CLP) in a rat model. Regarding the material, we used 20 adult-male Wistar rats (control group 1, n=10, and group 2, n=10, that underwent CLP protocol). The experimental protocol was approved by the National Sanitary Veterinary and Food Authority, Cluj (Romania), project number 116/11.05.2018. The endpoint of the experiment was pre-set to 10 hours post-surgery. A complete necropsy survey was performed. The main renal lesions detected histologically were: glomerular congestion and edema, hyalinization of glomerular mesangium, presence of hyaline in the urinary space with associated compression atrophy of vascular glomerular tuft, granules of hyaline in the lumen of cortical tubules, and reduction of the urinary space. The hepatic lesion identified histologically was represented by isolated miliary necrotic foci

Presence of Granular Ducts in Mandibular Gland in Rabbit

The present study focuses on the intralobular ducts present in rabbit mandibular gland, from a histological and histochemical point of view. We harvested mandibular gland samples from five rabbits (approximately six month old), which were paraffin embedded and subsequently stained for histological investigation with hematoxylin-eosin. PAS and Alcian blue reactions were used for histochemical assessment. Results show that mandibular gland in rabbit contains one type of acini, namely serous. Concerning the intralobular ducts, there were three types identified: intercalated, granular and striated. Granules present in the cytoplasm of the cells lining the granular ducts appear acidophilic on hematoxylin-eosin staining procedure. Histochemically, granular cells present a moderately PAS positive material (meaning they secrete neutral mucosubstances) and negative staining to Alcian blue reaction (no acid and sulfated mucosubstances were detected). We highlighted the presence of granular ducts in rabbit mandibular gland, which synthesize neutral mucosubstances according to the histochemical reactions applied

Implication of Utilizing Phytoestrogens Infested Fodder on Fertility and Histological Structure of Ovaries and Oviduct in Sow

Reproductive efficiency is an essential requirement for the profitability of swine farms and the quality of fodder can have a negative impact on this aspect. We carried out investigations regarding the influence of fodder quality on reproductive efficiency and the histological structure of the ovary and oviduct in 45 sows from a farm in Bihor county. We observed that corn utilized in feeding the sows was stored in inadequate conditions which allowed the development of moulds. The combined fodder, resulted after grinding the cereals, was stored directly on the concrete floor in inappropriate areas, concerning the hygiene. The animals were given green fodder directly on the stall’s floor, which favours mould expansion. Upon histological examination, the ovary presented an exaggerated activity and there was a marked congestion in the oviduct, with a tendency of the epithelium toward pseudostratification. The precarious conditions of cereal and combined fodder storing and administration of green fodder, brought optimum conditions for mould development. Under the action of the latter factors, the reproductive performance drastically decreased along with compromising the sows used for reproduction

STRUCTURAL PARTICULARITIES OF THE EPITHELIUM LINING THE LAMB EPIGLOTTIS

Epiglottis presents a central axis covered by mucosa. The aim of this study was highlighting the type of epithelium lining the lamb epiglottis. Thus, we histologically processed the epiglottis from 3 lambs. The epithelium lining the whole epiglottis surface is non-keratinized stratified squamous, with different thickness from one side to the other. Hence, on the pharyngeal side the epithelium is twice as thick as the one found on the laryngeal side

Intraspecific Differences Regarding Granular Polymorphism in Granular Ducts’ Cells in Rats’ Mandibular Gland

Mandibular gland ducts’ system in rodents consist of intralobular ducts (intercalated, granular, striated) and interlobular one (main excretory duct). Granular ducts are located between intercalated and striated ducts, being present only in mandibular gland of the mouse, rat, hamster and gerbil. The biological material used for this study was represented by two strains from the same species, three Wistar rats and three Brown Norway rats. After the animals were euthanized, the mandibular glands were harvested and then processed for histological investigations. The tissue fragments were sectioned at 5μm thickness and then stained the sections using Tricrom-Goldners method. Our results emphasize that the granular ducts are well developed; regarding the shape, they are convoluted in both Wistar and Brown Norway rats, without any significant differences between the two strains. In Wistar rat, the granules in granular ducts cells are small to medium in size, with discrete polymorphism. In Brown Norway rat, the cytoplasm is loaded with granules as in Wistar rat, but these are several times larger and more polymorphic