Transdisciplinary and Community Literacies: Shifting Discourses and Practices through New Paradigms of Public Scholarship and Action- Oriented Research

In 2010, we received a nationally competitive grant from the Ford Foundation to undertake cross-disciplinary, community-engaged work to shift public conversations around youth sexuality, health, and rights (YSHR). We came to the projects from our positions as a humanities scholar (Licona) and as a social science scholar (Russell). According to the Ford Foundation, “a deeper understanding of human sexuality is an essential element of human rights and healthy social relationships.” Beginning with this assumption, we seek to be informed by and to inform policies and local practices; to initiate broad conversations that address sexual health and healthy sexualities for youth; and ultimately to develop innovative collaborations, programs, and research

The Benefits of Re-Evaluating Real-Time Fulfillment Decisions

At the time of a customer order, the e-tailer assigns the order to one or more of its order fulfillment centers, and/or to drop shippers, so as to minimize procurement and transportation costs, based on the available current information. However this assignment is necessarily myopic as it cannot account for all future events, such as subsequent customer orders or inventory replenishments. We examine the potential benefits from periodically re-evaluating these real-time order-assignment decisions. We construct near-optimal heuristics for the re-assignment for a large set of customer orders with the objective to minimize the total number of shipments. We investigate how best to implement these heuristics for a rolling horizon, and discuss the effect of demand correlation, customer order size, and the number of customer orders on the nature of the heuristics. Finally, we present potential saving opportunities by testing the heuristics on sets of order data from a major e-tailer.Singapore-MIT Alliance (SMA

Traditional Inventory Models in an E-Retailing Setting: A Two-Stage Serial System with Space Constraints

In an e-retailing setting, the efficient utilization of inventory, storage space, and labor is paramount to achieving high levels of customer service and company profits. To optimize the storage space and labor, a retailer will split the warehouse into two storage regions with different densities. One region is for picking customer orders and the other to hold reserve stock. As a consequence, the inventory system for the warehouse is a multi-item two-stage, serial system. We investigate the problem when demand is stochastic and the objective is to minimize the total expected average cost under some space constraints. We generate an approximate formulation and solution procedure for a periodic review, nested ordering policy, and provide managerial insights on the trade-offs. In addition, we extend the formulation to account for shipping delays and advanced order information.Singapore-MIT Alliance (SMA

QUANTITATIVE ANALYSIS OF REPAIRED AND UNREPAIRED DAMAGE TO TRILOBITES FROM THE CAMBRIAN (STAGE 4, DRUMIAN) IBERIAN CHAINS, NE SPAIN

Repaired fossil skeletons provide the opportunity to study predation rates, repair mechanisms, and ecological interactions in deep time. Trilobites allow the study of repaired damage over long time periods and large geographic areas due to their longevity as a group, global distribution, and well-preserved mineralized exoskeletons. Repair frequencies on trilobites from three sites representing offshore marine environments in the Iberian Chains (Spain) show no injuries on 45 complete redlichiid thoraces from Minas Tierga (Huérmeda Formation, Cambrian Series 2, Stage 4), or 23 complete Eccaparadoxides pradoanus thoraces from Mesones de Isuela (Murero Formation, Cambrian Series 3, Drumian). Ten injuries on 69 E. pradoanus thoraces from Purujosa (Murero Formation, Cambrian Series 3, Drumian) were noted. There is no evidence for laterally asymmetric predation or size selection on the trilobites in this study. Weak evidence for selection for the rear of the thorax is documented. A series of injured trilobites illustrates four stages of the healing process. Analysis of injury locations and frequency suggests that injuries to these trilobites are predatory in origin. Semilandmark analysis of previously described exoskeletons with unrepaired damage assigned to the ichnotaxon Bicrescomanducator serratus alongside newly collected damaged exoskeletons from Purujosa (Mansilla and Murero Formations, Stage 5, Drumian), Mesones de Isuela (Murero Formation, Drumian), and Minas Tierga (Huérmeda Formation, Stage 4) found that shapes of biotic and abiotic breaks could not be distinguished.Department of Zoology, University of Oxford, Reino UnidoInstitute of Earth Sciences, University of Lausanne, SuizaPaleoscience Research Centre School of Environmental and Rural Science, University of New England, AustraliaUnidad de Zaragoza, Instituto Geológico y Minero de España, EspañaUnidad Asociada en Ciencias de la Tierra, Universidad de Zaragoza, Españ

Processing The Interspecies Quorum-Sensing Signal Autoinducer-2 (AI-2) Characterization Of Phospho-(S)-4,5-Dihydroxy-2,3-Pentanedione Isomerization By LsrG Protein

The molecule (S)-4,5-dihydroxy-2,3-pentanedione (DPD) is produced by many different species of bacteria and is the precursor of the signal molecule autoinducer-2 (AI-2). AI-2 mediates interspecies communication and facilitates regulation of bacterial behaviors such as biofilm formation and virulence. A variety of bacterial species have the ability to sequester and process the AI-2 present in their environment, thereby interfering with the cell-cell communication of other bacteria. This process involves the AI-2-regulated lsr operon, comprised of the Lsr transport system that facilitates uptake of the signal, a kinase that phosphorylates the signal to phospho-DPD (P-DPD), and enzymes (like LsrG) that are responsible for processing the phosphorylated signal. Because P-DPD is the intracellular inducer of the lsr operon, enzymes involved in P-DPD processing impact the levels of Lsr expression. Here we show that LsrG catalyzes isomerization of P-DPD into 3,4,4-trihydroxy-2-pentanone-5-phosphate. We present the crystal structure of LsrG, identify potential catalytic residues, and determine which of these residues affects P-DPD processing in vivo and in vitro. We also show that an lsrG deletion mutant accumulates at least 10 times more P-DPD than wild type cells. Consistent with this result, we find that the lsrG mutant has increased expression of the lsr operon and an altered profile of AI-2 accumulation and removal. Understanding of the biochemical mechanisms employed by bacteria to quench signaling of other species can be of great utility in the development of therapies to control bacterial behavior

Exploration of a Polarized Surface Bidirectional Reflectance Model Using the Ground-Based Multiangle Spectropolarimetric Imager

Accurate characterization of surface reflection is essential for retrieval of aerosols using downward-looking remote sensors. In this paper, observations from the Ground-based Multiangle SpectroPolarimetric Imager (GroundMSPI) are used to evaluate a surface polarized bidirectional reflectance distribution function (PBRDF) model. GroundMSPI is an eight-band spectropolarimetric camera mounted on a rotating gimbal to acquire pushbroom imagery of outdoor landscapes. The camera uses a very accurate photoelastic-modulator-based polarimetric imaging technique to acquire Stokes vector measurements in three of the instrument's bands (470, 660, and 865 nm). A description of the instrument is presented, and observations of selected targets within a scene acquired on 6 January 2010 are analyzed. Data collected during the course of the day as the Sun moved across the sky provided a range of illumination geometries that facilitated evaluation of the surface model, which is comprised of a volumetric reflection term represented by the modified Rahman-Pinty-Verstraete function plus a specular reflection term generated by a randomly oriented array of Fresnel-reflecting microfacets. While the model is fairly successful in predicting the polarized reflection from two grass targets in the scene, it does a poorer job for two manmade targets (a parking lot and a truck roof), possibly due to their greater degree of geometric organization. Several empirical adjustments to the model are explored and lead to improved fits to the data. For all targets, the data support the notion of spectral invariance in the angular shape of the unpolarized and polarized surface reflection. As noted by others, this behavior provides valuable constraints on the aerosol retrieval problem, and highlights the importance of multiangle observations.NASAJPLCenter for Space Researc

Promoter hypomethylation of NY-ESO-1, association with clinicopathological features and PD-L1 expression in non-small cell lung cancer.

Cancer-Testis antigens (CTA) are immunogenic molecules with normal tissue expression restricted to testes but with aberrant expression in up to 30% of non-small cell lung cancers (NSCLCs). Regulation of CTA expression is mediated in part through promoter DNA methylation. Recently, immunotherapy has altered treatment paradigms in NSCLC. Given its immunogenicity and ability to be re-expressed through demethylation, NY-ESO-1 promoter methylation, protein expression and its association with programmed death receptor ligand-1 (PD-L1) expression and clinicopathological features were investigated. Lung cancer cell line demethylation resulting from 5-Aza-2'-deoxycytidine treatment was associated with both NY-ESO-1 and PD-L1 re-expression in vitro but not increased chemosensitivity. NY-ESO-1 hypomethylation was observed in 15/94 (16%) of patient samples and associated with positive protein expression (P < 0.0001). In contrast, PD-L1 expression was observed in 50/91 (55%) but strong expression in only 12/91 (13%) cases. There was no association between NY-ESO-1 and PD-L1 expression, despite resultant re-expression of both by 5-Aza-2'-deoxycytidine. Importantly, NY-ESO-1 hypomethylation was found to be an independent marker of poor prognosis in patients not treated with chemotherapy (HR 3.59, P = 0.003) in multivariate analysis. In patients treated with chemotherapy there were no differences in survival associated with NY-ESO-1 hypomethylation. Collectively, these results provided supporting evidence for the potential use of NY-ESO-1 hypomethylation as a prognostic biomarker in stage 3 NSCLCs. In addition, these data highlight the potential to incorporate demethylating agents to enhance immune activation, in tumours currently devoid of immune infiltrates and expression of immune checkpoint genes

Coherent Detector for Near-Angle Scattering and Polarization Characterization of Telescope Mirror Coatings

A report discusses the difficulty of measuring scattering properties of coated mirrors extremely close to the specular reflection peak. A prototype Optical Hetero dyne Near-angle Scatterometer (OHNS) was developed. Light from a long-coherence-length (>150 m) 532-nm laser is split into two arms. Acousto-optic modulators frequency shift the sample and reference beams, establishing a fixed beat frequency between the beams. The sample beam is directed at very high f/# onto a mirror sample, and the point spread function (PSF) formed after the mirror sample is scanned with a pinhole. This light is recombined by a non-polarizing beam splitter and measured through heterodyne detection with a spectrum analyzer. Polarizers control the illuminated and analyzed polarization states, allowing the polarization dependent scatter to be measured. The bidirectional reflective or scattering distribution function is normally measured through use of a scattering goniometer instrument. The instrumental beam width (collection angle span) over which the scatterometer responds is typically many degrees. The OHNS enables measurement at angles as small as the first Airy disk diameter

Method and Apparatus for Measuring Near-Angle Scattering of Mirror Coatings

Disclosed herein is a method of determining the near angle scattering of a sample reflective surface comprising the steps of: a) splitting a beam of light having a coherence length of greater than or equal to about 2 meters into a sample beam and a reference beam; b) frequency shifting both the sample beam and the reference beam to produce a fixed beat frequency between the sample beam and the reference beam; c) directing the sample beam through a focusing lens and onto the sample reflective surface, d) reflecting the sample beam from the sample reflective surface through a detection restriction disposed on a movable stage; e) recombining the sample beam with the reference beam to form a recombined beam, followed by f) directing the recombined beam to a detector and performing heterodyne analysis on the recombined beam to measure the near-angle scattering of the sample reflective surface, wherein the position of the detection restriction relative to the sample beam is varied to occlude at least a portion of the sample beam to measure the near-angle scattering of the sample reflective surface. An apparatus according to the above method is also disclosed