Isolation And Structural Elucidation Of Two New Compound Munduleaxanthone And Jipaflavonone From Mundulea Antanossarum Baill. (Syn. Mundulea Anceps Var. Mangokyensis "R.Vig. P.P.A, Leguminosae) Originated From Madagascar

Mundulea antanossarum (Leguminosae), known by its vernacular name "Malaignarety", is a species of plant endemic to the southern part of Madagascar. It is a very important plant for society in this region, due to its therapeutic virtues in traditional Malagasy medicine.Phytochemical studies carried out on the pith of this plant isolated two pure products, and their chemical structures were elucidated using mass spectrometry (TOF-SM-EIS), ultra-violet visible (HPLC-UV-DAD), infra-red (IR) and nuclear magnetic resonance spectroscopy (1D and 2D NMR).The results of our studies carried out on this plant led to the discovery of two new molecules, Munduleaxanthone and Jipaflavonone, included in the flavonone chemical family with a reputation for biological activity.Key words: Munduleaxanthone, Jipaflavonone, Mundulea antanossarum, Phytochemistry, NMR (1D and 2D) and TOF-SM-EIS.

Healing Activity of Sepia Officinalis Bone

Madagascar is a country renowned worldwide for its exceptional biodiversity and high level of endemism. Its fauna is of particular scientific interest in the search for new molecules of biopharmaceutical interest. Surveys carried out in the south-west region of this large island have revealed that S. officinalis bones have been used in this region to treat wounds. Thanks to a number of biological models, this information on ethno-medical uses has been scientifically validated. The results of scientific studies show that it has two effects: healing and anti-healing

Antihypertenseur, Activité Vasorelaxant De Rouppelinia Boivinni (Apocynaceae) Et Etude De Relation Structure-Activité Des Alcaloïdes Indoliques Isolées

In the southern and south-western regions of Madagascar, traditional medicine plays an important role in society. Ethnobotanical surveys carried out in these regions have revealed that the plant known under the vernacular name "Feka (nom Mahafaly, Masikoro); Fekambato (Nom Antandroy); Fekakely (Nom Sakalava)" is used by the local population to treat hypertension. The results of biological tests (in-vivo and in-vitro) carried out on the alkaloid extract show improved vasorelaxant activity. These results justify their therapeutic virtues. Bioguided fractionation of the alkaloid extract from the aerial part of this plant resulted in the isolation of two pure products, PX-1 and PX-2. The chemical structures of these two pure products were isolated using spectral analysis methods. The ultraviolet spectrum of the PX-1 molecule recorded by LHPC-UV-DAD shows an absorption maximum at 240nm and 317nm, indicating the presence of an acylindole chromophore in PX-1. The peak at Tr= 2.9min corresponds to the molecular ion peak at m/z =355.20141 [M+H]+, corresponding to the empirical formula C21H26N2O3. Product PX-2 has a molecular weight of m/z = 352.17896 [M+H]+, corresponding to the molecular formula C21H24N2O3, and its UV spectrum recorded by HPLC-UV-DAD shows absorption maxima at 240nm and 317nm consistent with the 2-acyl indole chromophore. Analysis by nuclear magnetic resonance spectroscopy (1D and 2D NMR) confirms that the basic structures of PX-1 and PX-2 are attributed to indole alkaloids. Analysis of the pharmacological mechanism of this plant's vasorelaxant activity shows that the relaxant activity may result from blockade of calcium influx through vascular smooth muscle cells, as the contraction produced by CaCl2 on the aorta in a calcium-free depolarizing medium was inhibited by the alkaloid extract in a concentration-dependent manner with EC50= 0.061±0.0056mg/ml at n=7. Preliminary acute and chronic toxicity studies carried out on the alkaloid extract of this plant showed no significant toxic signs. In perspective, the study of the plant used in medicine to treat TAH enables the isolation of two indole-type alkaloids and the discovery of their mechanisms of pharmacological activity. It's time for researchers to turn to traditional medicine as an inexhaustible source of scientific discovery

Isolation and structural elucidation of cytotoxic compounds from the root bark of Diospyros quercina (Baill.) endemic to Madagascar

Objective: To isolate and characterize the cytotoxic compounds from Diospyros quercina (Baill.) G.E. Schatz & Lowry (Ebenaceae). Methods: An ethno-botanical survey was conducted in the south of Madagascar from July to August 2010. Bio-guided fractionation assay was carried out on the root bark of Diospyros quercina, using cytotoxicity bioassay on murine P388 leukemia cell lines as model. The structures of the cytotoxic compounds were elucidated by 1D and 2D NMR spectroscopy and mass spectrometry. Results: Biological experiments resulted in the isolation of three bioactive pure compounds (named TR-21, TR-22, and TR-23) which exhibited very good in vitro cytotoxic activities with the IC50 values of (0.0175±0.0060) μg/mL, (0.089±0.005) μg/mL and (1.027±0.070) μg/mL respectively. Thus, they support the claims of traditional healers and suggest the possible correlation between the chemical composition of this plant and its wide use in Malagasy folk medicine to treat cancer. Conclusions: The ability of isolated compounds in this study to inhibit cell growth may represent a rational explanation for the use of Diospyros quercina root bark in treating cancer by Malagasy traditional healers. Further studies are, therefore, necessary to evaluate the in vivo anti-neoplastic activity of these cytotoxic compounds as effective anticancer drugs