Study on Influencing Factors of Ground Pressure Behavior in Roadway-Concentrated Areas under Super-Thick Nappe

During the mining activity under the super-thick nappe formed by thrust fault, the law of mine pressure behavior is complex, and it is difficult to control the deformation and failure of surrounding rock. Combined with the actual engineering conditions, the influence of different roof lithology conditions, the thickness of nappe, the mining height, the size of the barrier coal pillar, and the creep time on mine pressure behavior was studied by UDEC numerical simulation software. The results showed that with the advancement of the coal face, due to the influence of the mining of the coal face and the slip dislocation of the super-thick nappe along the thrust faults, the roof-to-floor convergence, the two-sided convergence, and the maximum concentrated stress in the roadway-concentrated areas are significantly increased. For the above five influencing factors, the greater the thickness of the nappe and the mining height, the longer the creep time, and the stronger the ground pressure behavior. The larger the size of the barrier coal pillar, the stronger the roof lithology, and the gentler the ground pressure behavior. The research results can provide some reference for monitoring the law of ground pressure behavior in roadway-concentrated areas under super-thick nappe

Driving mechanisms of community biomass allocation along environmental gradients in different grasslands in China

A variable future climate would lead to changes in the biomass allocation pattern of plant communities, but its divergent changes under different environments have not been clearly explored, greatly limiting a better understanding of the responsess of grassland ecosystems to environmental changes. Herein, we conducted field samplings at 215 grassland sites in the Qinghai-Tibetan and Inner Mongolian Plateaus to analyze community biomass allocation patterns (specifically, the ratio of root to shoot, R/S) in various grasslands along environmental gradients. We found a gradual increase in R/S from humid to semi-arid and arid environments across both plateaus. In humid environment, soil factor had a significant effect on the R/S of alpine humid grassland, while climate factor had a significant effect on the R/S of humid grassland. In semi-arid environment, both climate and plant factors had significant effects on R/S in semi-arid grassland. In arid environments, climate and plant factors had significant effects on R/S in arid grassland. These findings emphasize the multifaceted role of environmental factors in shaping biomass allocations across diverse ecosystems, enriching our understanding of ecological dynamics. The current results are timely in advancing the fundamental understanding of the mechanisms of biomass allocation in grasslands and are useful for global research on grasslands in different environments

α-Synuclein Aggregates in the Nigro-Striatal Dopaminergic Pathway Impair Fine Movement: Partial Reversal by the Adenosine A_2A Receptor Antagonist

Parkinson’s disease (PD) is characterized pathologically by abnormal aggregation of alpha-synuclein (α-Syn) in the brain and clinically by fine movement deficits at the early stage, but the roles of α-Syn and associated neural circuits and neuromodulator bases in the development of fine movement deficits in PD are poorly understood, in part due to the lack of appropriate behavioral testing paradigms and PD models without motor confounding effects. Here, we coupled two unique behavioral paradigms with two PD models to reveal the following: (i) Focally injecting α-Syn fibrils into the dorsolateral striatum (DLS) and the transgenic expression of A53T-α-Syn in the dopaminergic neurons in the substantia nigra (SN, PITX3-IRES2-tTA/tetO-A53T mice) selectively impaired forelimb fine movements induced by the single-pellet reaching task. (ii) Injecting α-Syn fibers into the SN suppressed the coordination of cranial and forelimb fine movements induced by the sunflower seed opening test. (iii) Treatments with the adenosine A2A receptor (A2AR) antagonist KW6002 reversed the impairment of forelimb and cranial fine movements induced by α-Syn aggregates in the SN. These findings established a causal role of α-Syn in the SNc-DLS dopaminergic pathway in the development of forelimb and cranial fine movement deficits and suggest a novel therapeutic strategy to improve fine movements in PD by A2AR antagonists

Solubilization of Single-walled Carbon Nanotubes with Single- stranded DNA Generated from Asymmetric PCR

Carbon nanotubes (CNTs) can be effectively dispersed and functionalized bywrapping with long single-stranded DNA (ssDNA) synthesized by asymmetric PCR. ThessDNA-CNTs attached on surface of glass carbon electrode made it possible forelectrochemical analysis and sensing, which was demonstrated by reduction of H2O2 onhemoglobin/ssDNA-CNTs modified electrodes. This research showed the potentialapplication of DNA-functionalised CNTs in construction of future electrochemicalbiosensors