Circulating microparticles: square the circle

Background: The present review summarizes current knowledge about microparticles (MPs) and provides a systematic overview of last 20 years of research on circulating MPs, with particular focus on their clinical relevance. Results: MPs are a heterogeneous population of cell-derived vesicles, with sizes ranging between 50 and 1000 nm. MPs are capable of transferring peptides, proteins, lipid components, microRNA, mRNA, and DNA from one cell to another without direct cell-to-cell contact. Growing evidence suggests that MPs present in peripheral blood and body fluids contribute to the development and progression of cancer, and are of pathophysiological relevance for autoimmune, inflammatory, infectious, cardiovascular, hematological, and other diseases. MPs have large diagnostic potential as biomarkers; however, due to current technological limitations in purification of MPs and an absence of standardized methods of MP detection, challenges remain in validating the potential of MPs as a non-invasive and early diagnostic platform. Conclusions: Improvements in the effective deciphering of MP molecular signatures will be critical not only for diagnostics, but also for the evaluation of treatment regimens and predicting disease outcomes

Alignment of the optical feedback system of VUV regenerative FEL amplifier at the TESLA test facility at DESY

In this paper, we describe optical feedback system of VUV Regenerative FEL Amplifier (RAFEL) at the TESLA test facility at DESY. The aim of the RAFEL experiment is to construct fully coherent, tunable VUV radiation source by means of applying narrow-band optical feedback in the VUV SASE FEL operating currently at DESY. One of the problem of the realization of the RAFEL is severe requirements for the angular stability of the optical elements (about few microradians). This problem has been solved by means of installation of active alignment system with reference laser. Another problem is alignment of optical elements separated by 65 m within complicated experimental conditions connected with aperture limitations (down to 6 mm ) . This problem has been solved in two steps. Preliminary alignment with an accuracy of about 80 μrad has been performed with laser alignment system and OTR screens used at the TTF accelerator for electron beam diagnostics. Final alignment has been performed with VUV SASE FEL radiation. Measured feedback coefficient is about 1 percent and is in agreement with the designed value