IJCM_265A: Cellular Consequences of Fruit Juice Metallurgy: A Closer Look at Heavy Metal Influence

Background: The leaching of heavy metals from packaging materials into packed juices can have several adverse effects on the quality and safety of the products and potentially pose health risks to consumers. Objective: The research aimed to investigate the cytotoxic effects of heavy metals specifically vanadium, cadmium, arsenic, and mercury, which are commonly found in packaging materials of marketable fruit juices which are leached into juices itself (orange and mixed fruit juices stored in cans, leak- proof bags, and tetraPaks). Methodology: The study utilized cell lines, namely HepG2 (human liver cancer) and HeK293 (human kidney), to assess the impact of these heavy metals on cellular viability, DNA damage, mitochondrial function, and cell cycle progression. Flow cytometry was used to analyse cell cycle alterations. Results: The MTT assay showcased dose-dependent decrease in cell viability with increasing concentrations of heavy metals. Annexin V Assay determined V, Hg and As had necrotic death pattern while Cd had apoptotic death pattern for both the cell lines in presence of heavy metal digests. Cells treated with heavy metals, and the resulting DNA damage observed to be high after quantified using comet tail length, olive tail movement, and tail moment measurements. Mitochondrial function assays in the presence of heavy metals revealed that increased mitochondrial membrane potential, mitochondrial mass, and reactive oxygen species production. ROS generation assessed to understand the impact of heavy metals on cellular health and oxidative stress. Conclusion: This study shed light on the potential cytotoxic effects of heavy metals found in fruit juices. These findings contribute to our understanding of the potential risks associated with heavy metal exposure from consumer products and emphasize the importance of regulatory measures to ensure food safety and human health

Evaluation of Ischemia-Modified Albumin, Malondialdehyde, and Advanced Oxidative Protein Products as Markers of Vascular Injury in Diabetic Nephropathy

AIM This study aimed at evaluation of ischemia-modified albumin (IMA), malondialdehyde (MDA), and advanced oxidative protein products (AOPP) as markers of vascular injury in diabetic nephropathy (DN) with derivation of cutoff values for the same. Materials and Methods Study population comprised 60 diabetes patients and 30 controls, with diabetes patients further categorized into three groups based on urine albumin/creatinine ratio (UACR) of 300 mg/g of creatinine (overt DN). Serum IMA, MDA, and AOPP were estimated by enzyme-linked immunosorbent assay; HbA1c, serum creatinine, urine albumin, and urine creatinine were estimated using automated analyzers. Statistical analysis was done using analysis of variance, Pearson's correlation coefficient, and receiver-operating characteristic curve. Results A statistically significant difference was found in the levels of IMA among patients with early DN (154 ng/mL), diabetes without nephropathy (109.4 ng/mL), and healthy controls (45.7 ng/mL), with highest levels in early DN cases. Similar increase was seen in AOPP as well. A significant correlation was observed between IMA and UACR in diabetes without nephropathy ( r = 0.448). Conclusion The present study postulates serum IMA as a novel biomarker for the assessment of disease progression in diabetes even before microalbuminuria, and a cutoff point ≥99 ng/mL can be used for detection of early DN