Cutting edge: a critical role for CD70 in CD8 T cell priming by CD40-licensed APCs

The CD154/CD40 interaction is an important pathway of CD4 T cell help for CD8 T cell responses. In this study, we address the role of CD70, a member of the TNF superfamily and the ligand for the T cell costimulatory receptor CD27, in CD40-mediated priming of CD8 T cells. Using an agonistic anti-CD40 mAb to mimic the CD154/CD40 interaction we demonstrate that the priming of OT-I TCR transgenic or endogenous mouse OVA-specific CD8 T cells is critically dependent on CD70/CD27 interaction. CD70 blockade inhibited CD40-mediated clonal expansion of CD8 T cells and reduced the number of memory CD8 T cells generated. Furthermore, CD70 blockade during the initial priming of CD8 T cells inhibited the ability of memory CD8 T cells to expand in response to a second encounter with Ag. Our data indicate that CD70 expression on APCs plays a key role in CD40-dependent CD8 T cell responses

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10.1007/s00530-005-0167-6Multimedia Systems105379-391MUSY

CD27 costimulation contributes substantially to the expansion of functional memory CD8+ T cells after peptide immunization

Naive T cells require signals from multiple costimulatory receptors to acquire full effector function and differentiate to long-lived memory cells. The costimulatory receptor, CD27, is essential for optimal T-cell priming and memory differentiation in a variety of settings although whether CD27 is similarly required during memory CD8+ T-cell re-activation remains controversial. We have used OVA and anti-CD40 to establish a memory CD8+ T-cell population and report here that their secondary expansion, driven by peptide and anti-CD40, polyI:C or LPS requires CD27. Furthermore, antigenic peptide and a soluble form of the CD27 ligand, CD70 (sCD70), is sufficient for secondary memory CD8+ T-cell accumulation at multiple anatomical sites, dependent on CD80/86. Prior to boost, resting effector- and central-memory CD8+ T cells both expressed CD27 with greater expression on central memory cells. Nonetheless both populations upregulated CD27 after TCR engagement and accumulated in proportion after boosting with antigen and sCD70. Mechanistically, sCD70 increased the frequency of divided and cytolytic memory T cells, conferred resistance to apoptosis and enabled retardation of tumor growth in vivo. These data demonstrate the central role played by CD27/70 during secondary CD8+ T-cell activation to a peptide antigen, and identify sCD70 as an immunotherapeutic adjuvant for anti-tumor immunit

Integrin beta2-phosphorylation on Thr758 acts as a molecular switch to regulate 14-3-3 and filamin binding

This paper looks at the effects of dust allergen and LPS concentrations on biomarkers of asthma in adults with house dust mite-sensitive asthma

A Benchmark for Geometric Facial Beauty Study

2nd International Conference on Medical Biometrics, ICMB 2010, Hong Kong, 28-30 June 2010This paper presents statistical analyses for facial beauty study. A large-scale database was built, containing 23412 frontal face images, 875 of them are marked as beautiful. We focus on the geometric feature defined by a set of landmarks on faces. A normalization approach is proposed to filter out the non-shape variations - translation, rotation, and scale. The normalized features are then mapped to its tangent space, in which we conduct statistical analyses: Hotelling's T2 test is applied for testing whether female and male mean faces have significant difference; Principal Component Analysis (PCA) is applied to summarize the main modes of shape variation and do dimension reduction; A criterion based on the Kullback-Leibler (KL) divergence is proposed to evaluate different hypotheses and models. The KL divergence measures the distribution difference between the beautiful group and the whole population. The results show that male and female faces come from different Gaussian distributions, but the two distributions overlap each other severely. By measuring the KL divergence, it shows that multivariate Gaussian model embodies much more beauty related information than the averageness hypothesis and the symmetry hypothesis. We hope the large-scale database and the proposed evaluation methods can serve as a benchmark for further studies.Department of Computin