4 research outputs found
Characterization and molecular diversity of Iranian rhizobia isolated from faba bean
The diversity and phylogeny of 30 rhizobia isolated from nodules of faba bean
plants grown on 5 geographic regions located in the East Azerbaijan province
of Iran were examined using rep-PCR fingerprinting, sequence analysis of 16S
rRNA accompanied with nodC genes. Based on cluster analysis of rep-PCR
fingerprints, faba bean rhizobia isolates were differentiated into five
clusters (A to E) at 80% similarity level. The cophenetic correlation
coefficient for the dendrogram obtained from the combined dataset of BOX and
ERIC primers was 0.942. The percentage of polymorphic loci was 59.2% using
the BOX-PCR primer and 67.3% using the ERIC-PCR primers. The data obtained
by rep-PCR fingerprinting showed high apparent correlation between genetic
diversity and geographical origin of the isolates. The phylogenetic analysis
based on 16S rRNA and nodC sequences showed that representative isolates
were closely related to R. leguminosarum bv. viciae and R. fabae. To the
best of our knowledge, this is first report of isolation and
characterization of R. fabae from Iran