Effect of Brown Algae Cystoseira trinodis Methanolic Extract on Renal Tissue

Background: C.trinodisis brown algae of Oman Sea coast is used traditionally as a diuretic in Chabahar, Sistan and Baluchestan province of Iran. But no researches have been conducted on the distractive effects of this alga on the renal tissues until now. Methods: Forty-two adult male mice were divided into 6 groups. Control group received normal saline (E0), group (E1) treated with 5mg/kg methanolic extract (ME) and group (E2) to (E5) received 10, 15, 25 and 50 mg/kg of ME of alga respectively. All animals in 6 groups were treated for 2 weeks (once every other day). Finally, histopathological evaluations were made especially by morphology and photometric method. Results: ME of C.trinodis induced histological damage in kidney. Administration of ME in all experimental groups induced severe glomerular congestion, hyaline cast and severe interstitial inflammatory centers in treated groups. All distractive parameter in test groups increased with increasing dose of extract (p<0.05). Conclusion: Results showed that ME of the C.trinodis has a nephrotoxic effect on the renal tissues

Comparison of melatonin and curcumin effect at the light and dark periods on regeneration of sciatic nerve crush injury in rats

Being one of the acute clinical problems, peripheral nerve injury can bring about a number of consequences in- cluding severe disability, redu ced Quality of life (QOL) and immense costs. Currently, melatonin and curcumin are widely applied because of their immunomodulatory, anti-inflammatory, neuro-protective and antioxidant prop- erties. The present stud y aims to compare the effects of melatonin and curcumin during light and dark periods on sciatic nerve crush injury repair. Acco rdingly, rats received IP injections of curcumin (100 mg/kg) and melatonin (10 mg/kg) over two periods of light (9:00 a.m.) and dark (9 :00 p.m.) for 4 weeks. In order to evaluate rats, func- tional (walking track analysis and electrophysiological measurements), histomorphometric and gastrocnemius muscle mass investigations were administered. No statis tically significant difference was identified between dark and light curcumin groups while curcumin groups displayed better results than did melatonin groups. In addition, dark melatonin group displayed better results than the light melatonin. On the whole, this study found that mela- tonin and curcumin can be used to quicken neural recovery and help treat nerve injury. It was also found that better neuroregeneration or nerve regeneration was induced when ra ts were treated by melatonin during the dark period while effects and injection time did not correlate in curcum in application

Effect of Lamium Album on Mitochondrial Oxidative Stress in Diabetic Rats

Background: Diabetes mellitus (DM) is characterized by the presence of hyperglycemia. It has been documented that oxidative stress and reactive oxygen species (ROS) production have a key role in the pathogenesis of diabetes and its complications. Neutrophils as a part of immune system produce ROS, neutrophils function might be altered in diabetes. Lamium album is known to have antioxidant, and free radical scavenging actions. The aim of the present study was to evaluate the potential effect of L. album on mitochondrial ROS production from circulating neutrophils in diabetic rats. Materials and Methods: Twenty-one male Wistar rats were randomly divided into three groups: normal control rats receiving daily saline; diabetic control rats receiving daily saline; and diabetic rats treated daily with hydroalcoholic extract of L. album (100 mg/kg) for 28 days. On the 28thday of treatment, whole blood samples were obtained and mitochondrial ROS of neutrophils were measured by dihydrorhodamine (DHR) flow cytometric method. Also, fasting blood sugar (FBS) was measured. Results: Mitochondrial ROS didn&rsquo;t show any significant differences among diabetic rats treated with L. album extract, diabetic control rats, and normal control rats (P=0.8). Serum glucose in diabetic control was significantly higher than normal control rats (P=0.0001). However, L. album caused a remarkable decrease in serum glucose of diabetic rats (P=0.03). Conclusion: According to the present findings, it seems that L. album at a dose of 100 mg/kg could not decrease mitochondrial ROS production from neutrophils in diabetic rats. Further studies considering higher concentrations of L. album are appreciated to evaluate its impact on the production of mitochondrial ROS along with extracellular ROS in diabetes condition