IN VITRO CYTOSTATIC EFFECT OF SOME NON-IONIZING ELECTROMAGNETIC FIELDS

The in vitro action of some kinds of electromagnetic fields, generated by a magnetodiaflux apparatus, upon the proteinsynthesis of HeLa cancerous cells and of RM healthy renal cells, implicitly, as well as upon the development of the corresponding cellular cultures was investigated. The significant perturbation of proteic biogenesis, the modification of the protein dynamics, the inhibition of the cell cultures development and the existence of a dose-response relationship argue the behaviour of the low intensity and frequency electromagnetic field as in vitro active cytostatic agent. The registered data have revealed that the intensity of the induced biological effects of EMFs is dependent on the in vitro model, intensity and type of electromagnetic field, exposure time, metabolic state and type of the exposed cells. This primary characterization of the low intensity and frequency fields as cytostatic agent justifies the study of their effect upon cell proliferation and viability in order to enlarge the reasoning basis for the introduction of this physical agent in the in vivo antitumoral screening program on different experimental tumoral systems

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METABOLIC PROFILE OF THE NEOPLASTIC CELLS TREATED IN VITRO WITH ANTITUMORAL FUROSTANOLIC-GLYCOSIDE BIOPREPARATIONS

The in vitro short-lasting cytostatic treatment of the HeLa and HEp-2p tumoral cell cultures with some original furostanolic-glycoside biopreparations has conditioned the perturbation of the glucidic, lipidic and proteic intermediary metabolism processes and of the nucleic acids biochemistry. The metabolic profile of the treated cells seems to be of catabolic type, being outlined by enhancement of the glicogenolysis, glycolysis, lipolysis and proteolysis, of intensification of intracellular consumption of the glucose, lactic acid, free fatty acids and aminoacids, of inhibitory effect upon nucleic acids biosynthesis. These metabolic events were appreciated on the basis of the reduced contents of glycogen, glucose, lactic acid, total lipids, free fatty acids, soluble and unsoluble proteins, DNA and RNA biomolecules. The new tumoral cell metabolic behaviour induced by furostanolicglycoside cytostatics – analyzed in comparison with that of the control untreated tumoral cells – can be consequence of an interaction between the bioactive agents either with the membrane receptors or with intracellular receptors

The in vivo assessment of the preclinical oncochemotherapeutic effectivness of some furostanolic glycosides

The significance of the antitumoral action of some bio- or semiynthesis furostanolic-glycoside preparations upon the development of diverse experimental tumoral lines has been appreciated in relation to the therapeutic effect of different doses on carcinogenesis, as well as the experimental oncostatic activity of some standard cytostatics of clinical use (methotrexate, cyclophosfamide, melphalan and 5-fluorouracil). The laboratory treatment with the bioactive agent in various doses – superior and inferior to that which has conditioned the expression of those antitumoral actions upon Guerin T-8 lymphotropic epithelioma and Walker 256 carcinosarcoma – has highlighted the antineoplastic effectiveness` dependence of these agents of the therapeutic dose. The comparative analysis of the evaluation indices values of the antitumoral pharmacodynamic effect – registerd by us in the experimental therapy with the glycoside extracts and with the reference cytostatic drugs – has revealed that the antitumoral potential of the new autochthonous products is higher, equal or near to those of the standard oncochemotherapeutics. The possibility of optimization of the antitumoral efficiency by experimental manipulation of the therapeutic doses – which proves the existence of a dose-response relationship as well as the antineoplastic effectiveness are relevant for the characterization of the ENLE and D2ENLE as potential oncochemotherapeutic agents. The quantitative preclinical evaluation of the specific pharmacodynamic effect will be completed by the investigation of the new furostanolic-glycoside extracts in tumors with various degree of development

Cellular effects of some bioactive principles from red wines and must

The in vitro action of some samples of red wine and concentrated must on the liver and striated muscle of frog (Rana ridibunda, Pall) was studied, following the intensity of cellular respiration, and the redox potential (rH). Both wine (1 mL/100mL physiological solution normal Ringer – NR) and must (2 mg dry matter/100mL NR) stimulate aerobe cellular respiration (mm3 O2/g wet tissue) and diminish the redox potential values, comparatively with the non-treated reference sample. Also, on treating liver and muscle cells with ethanol (1 mL/100mL NR) an evident depression of cellular respiration and increase of redox potential values may be noticed, alongwith perturbation of cellular metabolic processes. The obtained results evidence possible energizing, hepatoprotecting and redox modulating properties of the bioactive principles from red wine and must

IN VITRO SELECTION OF SOME POTENTIAL CYTOSTATIC AGENTS FROM NEW FUNGAL EXOPOLYGLUCIDIC EXTRACTS

The in vitro action of some new autochthonous glucanic biopreparations, specifically extracted from diverse submerged strains of Claviceps purpurea, upon the proteinsynthesis of Hep-2p cancerous cells as well as upon the development of the corresponding cellular cultures was investigated. The significant perturbation of proteic biogenesis, the modification of the protein dynamics, the inhibition of the cell cultures development and the existence of a doseresponse relationship argue the behaviour of the low intensity and frequency electromagnetic field as in vitro active cytostatic agent. This primary characterization of some glucanic extracts as cytostatic agent justifies the study of their effect upon cell proliferation and viability in order to enlarge the reasoning basis for the introduction of those exopolysaccharidic agents in the in vivo antitumoral screening program on different experimental tumoral systems