Knowledge modelling with the open source tool myCBR

Building knowledge intensive Case-Based Reasoning applications requires tools that support this on-going process between domain experts and knowledge engineers. In this paper we will introduce how the open source tool myCBR 3 allows for flexible knowledge elicitation and formalisation form CBR and non CBR experts. We detail on myCBR 3 's versatile approach to similarity modelling and will give an overview of the Knowledge Engineering workbench, providing the tools for the modelling process. We underline our presentation with three case studies of knowledge modelling for technical diagnosis and recommendation systems using myCBR 3

Towards a new reconstruction of the text of Marcion’s Gospel : history of research, sources, methodology, and the Testimony of Tertullian

This thesis provides the initial and foundational steps for a new reconstruction of the text of Marcion’s Gospel. Though Harnack’s 1924 magisterial work on Marcion remains valuable and important, shortcomings in his reconstructed text of the Marcionite scriptures, as well as advances in critical methodology, text criticism, and patristic studies have led to the recognition that new reconstructions of Marcion’s scriptures are a scholarly desideratum. With the text of Marcion’s Apostolikon examined and reconstructed in a 1995 work by Ulrich Schmid, this thesis provides the most important elements for a new examination and reconstruction of Marcion’s Euangelion. Chapter 1 provides an extensive history of research, not only to provide the context and rationale for the present work, but also to provide the first in-depth scholarly survey of work on Marcion’s Gospel in 150 years. In addition, since several flaws in earlier studies arose out of a lack of an accurate understanding of the status quaestionis at various points in the history of research on Marcion’s Gospel, by considering and engaging with previous scholarship such errors can be avoided. Chapter 2 begins with a consideration of the sources for Marcion’s Gospel and provides a comprehensive listing of verses attested as present in, verses attested as absent from, and unattested verses of this Gospel. The chapter concludes with a methodological discussion, highlighting the particular importance of understanding the citation customs of the witnesses to Marcion’s text and noting the significant citation customs of Tertullian demonstrated by Schmid’s and my own research. Chapter 3 begins the analysis of the data found in Tertullian, the most extensive and important source for Marcion’s Gospel. This chapter examines all of the verses that Tertullian attests for Marcion’s Gospel that are also cited elsewhere in Tertullian’s corpus and focuses particularly on how these multiply-cited passages provide insight into Tertullian’s testimony to readings in Marcion’s text. Chapter 4 continues the analysis of Tertullian’s testimony by examining the remaining verses, i.e., those attested for Marcion’s Gospel but not multiply-cited in Tertullian’s corpus. Chapter 5 provides a reconstruction of the 328 verses in Marcion’s Gospel for which Tertullian is the only witness and offers not only readings for Marcion’s text, but also the relative certainty for those readings. Chapter 6 summarizes and concludes the thesis, along with brief mention of avenues for future research

Zur wahlsoziologischen Bedeutung eines Modells sozialstrukturell verankerter Konfliktlinien im vereinten Deutschland

Der von Stein Rokkan 1967 zusammen mit Seymour Martin Lipset herausgegebene Sammelband 'Party systems and voter alignments' ist zweifellos eines der einflußreichsten und meistzitierten Werke der Wahlsoziologie. Der vorliegende Beitrag geht der Frage nach, ob das von Rokkan und Lipset konzipierte Konfliktlinienmodell (cleavage) noch einen Beitrag zur Erklärung des Wahlverhaltens im neuen, größeren Deutschland leisten kann. Bei der Prüfung der Theorie stützen sich die Autoren hauptsächlich auf die Daten der Forschungsgruppe Wahlen und das ZDF-Politbarometer. Die gesellschaftlichen Subsysteme bergen nach Lipset/Rokkan folgende Konfliktpotentiale in sich, die in dem Parteiensystem ihren Niederschlag finden: die Spaltung Besitz-Arbeit, die Spaltung Staat-Kirche, die Spaltung Stadt-Land und die Ethnische/Linguistische Spaltung. Die Sekundäranalyse zeigt, daß die Theorie sozialstrukturell verankerter Konfliktlinien als Determinanten des Wahlverhaltens nach wie vor einen eigenständigen Beitrag zur Erklärung der individuellen Parteipräferenzen in Deutschland zu leisten vermag. (pmb)'Electoral behavior in West Germany on state and federal levels was characterized by remarkable stability until the late 80's. This could be explained for a long time by the so called 'cleavage theory' of Stein Rokkan and Seymour Martin Lipset which says that the more or less frozen party systems of western democraties are based on sociostructural conflicts in these societies. Since the late 80's we report a clear drop in the stability of voting results. In addition, the unification of the two german states raises the question whether the cleavage model still can explain individual party preferences although voters in West and East were brought up in very different social and political structures. To answer this question we analyse party preferences in West and East separately as well as for the whole unified Germany. It will be clear that even under the changed conditions of the united Germany the model of Lipset and Rokkan can still contribute an independent part of the explanation of voting behavior.' (author's abstract

Probleme und politische Einstellungen in Heidelberg

Umfrage zu Problemen und politischen Einstellungen in Heidelberg im Vorfeld der Kommunanal- und Europawahlen 1994. 662 Telefoninterviews

SEASALTexp - an explanation-aware architecture for extracting and case-based processing of experiences from internet communities

This paper briefly describes SEASALTexp, an extension of the application-independent SEASALT architecture (Sharing Experience using an Agent-based explanation-aware System Architecture LayouT), which offers knowledge acquisition from Internet communities, knowledge modularisation, and agent-based knowledge maintenance complemented with agent-based explanation facilities

Production of recombinant antibody fragments in Bacillus megaterium

BACKGROUND: Recombinant antibodies are essential reagents for research, diagnostics and therapy. The well established production host Escherichia coli relies on the secretion into the periplasmic space for antibody synthesis. Due to the outer membrane of Gram-negative bacteria, only a fraction of this material reaches the medium. Recently, the Gram-positive bacterium Bacillus megaterium was shown to efficiently secrete recombinant proteins into the growth medium. Here we evaluated B. megaterium for the recombinant production of antibody fragments. RESULTS: The lysozyme specific single chain Fv (scFv) fragment D1.3 was succesfully produced using B. megaterium. The impact of culture medium composition, gene expression time and culture temperatures on the production of functional scFv protein was systematically analyzed. A production and secretion at 41°C for 24 h using TB medium was optimal for this individual scFv. Interestingly, these parameters were very different to the optimal conditions for the expression of other proteins in B. megaterium. Per L culture supernatant, more than 400 μg of recombinant His(6)-tagged antibody fragment were purified by one step affinity chromatography. The material produced by B. megaterium showed an increased specific activity compared to material produced in E. coli. CONCLUSION: High yields of functional scFv antibody fragments can be produced and secreted into the culture medium by B. megaterium, making this production system a reasonable alternative to E. coli

Building case-based reasoning applications with myCBR and COLIBRI Studio

myCBR and COLIBRI Studio are two well-established opensource frameworks for building case-based reasoning (CBR) applications, though they follow different approaches and support different phases of the CBR application development. In a nutshell: Where myCBR supports its users in developing a knowledge model for representing cases, it more or less leaves the software developers alone when they try to develop an application that uses the generated knowledge model. COLIBRI Studio, on the other hand, is focused in the development of applications that use that knowledge model. As soon as you have a knowledge model COLIBRI Studio offers templates for a variety of application types and supports in generating its source code. This paper explains the strengths and weaknesses of both frameworks regarding the rapid development of CBR applications. It also shows how to use both of them in conjunction

The ERA2 facility: towards application of a fiber-based astronomical spectrograph for imaging spectroscopy in life sciences

Astronomical instrumentation is most of the time faced with challenging requirements in terms of sensitivity, stability, complexity, etc., and therefore leads to high performance developments that at first sight appear to be suitable only for the specific design application at the telescope. However, their usefulness in other disciplines and for other applications is not excluded. The ERA2 facility is a lab demonstrator, based on a high-performance astronomical spectrograph, which is intended to explore the innovation potential of fiber-coupled multi-channel spectroscopy for spatially resolved spectroscopy in life science, material sciences, and other areas of research.Comment: 10 pages, 9 figures, SPIE Conference "Astronomical Telescopes and Instrumentation" 2012, Amsterda

Active poly-GA vaccination prevents microglia activation and motor deficits in a C9orf72 mouse model

The C9orf72 repeat expansion is the most common genetic cause of amyotrophic lateral sclerosis (ALS) and/or frontotemporal dementia (FTD). Non-canonical translation of the expanded repeat results in abundant poly-GA inclusion pathology throughout the CNS. (GA)(149)-CFP expression in mice triggers motor deficits and neuroinflammation. Since poly-GA is transmitted between cells, we investigated the therapeutic potential of anti-GA antibodies by vaccinating (GA)(149)-CFP mice. To overcome poor immunogenicity, we compared the antibody response of multivalent ovalbumin-(GA)(10) conjugates and pre-aggregated carrier-free (GA)(15). Only ovalbumin-(GA)(10) immunization induced a strong anti-GA response. The resulting antisera detected poly-GA aggregates in cell culture and patient tissue. Ovalbumin-(GA)(10) immunization largely rescued the motor function in (GA)(149)-CFP transgenic mice and reduced poly-GA inclusions. Transcriptome analysis showed less neuroinflammation in ovalbumin-(GA)(10)-immunized poly-GA mice, which was corroborated by semiquantitative and morphological analysis of microglia/macrophages. Moreover, cytoplasmic TDP-43 mislocalization and levels of the neurofilament light chain in the CSF were reduced, suggesting neuroaxonal damage is reduced. Our data suggest that immunotherapy may be a viable primary prevention strategy for ALS/FTD in C9orf72 mutation carriers

Energy Estimation of Cosmic Rays with the Engineering Radio Array of the Pierre Auger Observatory

The Auger Engineering Radio Array (AERA) is part of the Pierre Auger Observatory and is used to detect the radio emission of cosmic-ray air showers. These observations are compared to the data of the surface detector stations of the Observatory, which provide well-calibrated information on the cosmic-ray energies and arrival directions. The response of the radio stations in the 30 to 80 MHz regime has been thoroughly calibrated to enable the reconstruction of the incoming electric field. For the latter, the energy deposit per area is determined from the radio pulses at each observer position and is interpolated using a two-dimensional function that takes into account signal asymmetries due to interference between the geomagnetic and charge-excess emission components. The spatial integral over the signal distribution gives a direct measurement of the energy transferred from the primary cosmic ray into radio emission in the AERA frequency range. We measure 15.8 MeV of radiation energy for a 1 EeV air shower arriving perpendicularly to the geomagnetic field. This radiation energy -- corrected for geometrical effects -- is used as a cosmic-ray energy estimator. Performing an absolute energy calibration against the surface-detector information, we observe that this radio-energy estimator scales quadratically with the cosmic-ray energy as expected for coherent emission. We find an energy resolution of the radio reconstruction of 22% for the data set and 17% for a high-quality subset containing only events with at least five radio stations with signal.Comment: Replaced with published version. Added journal reference and DO