Laboratory clinical assessment of patients with AML over six years (2010-15) in Hematology -Oncology of Namazi Hospital in Shiraz

Introduction: Acute myeloid leukemia is a group of neoplasm of the bone marrow that hasclinical similarity but has but a specific cellular morphology and genetics.AML includes about 90-80% of adult acute leukemia and is the third leading cause of death due to cancer in people aged 30-18 years old. So, this study aims to examine patients with AML in a clinical laboratory manner who have been diagnosed over six years (2010-15) in Hematology-Oncology section of Namazi hospital and admitted there.Method: By using acute myeloid leukemia patients' records who had been hospitalized six years (94-89) in Hematology-Oncology section of Namazi hospital, this study was performed retrospectively.The necessary data included age, sex, diagnosing leukemia type, place of birth, occupation, time of admission and discharge, duration of hospitalization, the patient's chief complaint and other complaints, the duration of the preliminary symptoms prior to diagnosis, history of smoking and exposure to radiation or chemicals, physical examination findings and laboratory findings including blood chemistry and bone marrow, study of cerebrospinal fluid in case it had been done, positive culture and information relating to the treatment of early response, duration of remission, complications of induction of remission and the time of  recurrence and death and the cause of death, all of which were recorded in a form that was attached. The results of this study are indicated in an observational way. But, wherever it was possible, comparison was done and this comparison was evaluated using Z test. P <0.05 is significant.Results: During this period, 244 patients were diagnosed and hospitalized that 141 of them (58%) were male and 103 (42%) were female. Then, M1 with (20%), M2 (6/19%) and M3 with 18.3% are ordered. Finally M5 with 8%,and M7 and Hybrid each with 1.2% and M6 with 0.8% are classified in the next ranks.Most patients were from the age group of 29-20 years. Regardless of the type of leukemia and sex,the average age of our patients was 36/17 years (SD 16/11 ± years). The worst prognosis of M3 was death initial response to chemotherapyin 52.5% and the best prognosis of M2 was death as the initial response to treatment in 27.5%. The most common cause of death in our patients was first bleeding with 38.5% and then infection with 27%.Conclusion: Acute myeloid leukemia, unlike in other parts of the world,is still a lethal disease with anominous sequel among our young population. Swollen lymph glands and organs are more common in patients. A closer examination and laboratory examination, at least a complete blood test in suspected patients could be life-saving.Keywords: AML, Hematology, Oncolog

Genetic variability of Taenia saginata inferred from mitochondrial DNA sequences

Taenia saginata is an important tapeworm, infecting humans in many parts of the world. The present study was undertaken to identify inter- and intraspecific variation of T. saginata isolated from cattle in different parts of Iran using two mitochondrial CO1 and 12S rRNA genes. Up to 105 bovine specimens of T. saginata were collected from 20 slaughterhouses in three provinces of Iran. DNA were extracted from the metacestode Cysticercus bovis. After PCR amplification, sequencing of CO1 and 12S rRNA genes were carried out and two phylogenetic analyses of the sequence data were generated by Bayesian inference on CO1 and 12S rRNA sequences. Sequence analyses of CO1 and 12S rRNA genes showed 11 and 29 representative profiles respectively. The level of pairwise nucleotide variation between individual haplotypes of CO1 gene was 0.3–2.4 % while the overall nucleotide variation among all 11 haplotypes was 4.6 %. For 12S rRNA sequence data, level of pairwise nucleotide variation was 0.2–2.5 % and the overall nucleotide variation was determined as 5.8 % among 29 haplotypes of 12S rRNA gene. Considerable genetic diversity was found in both mitochondrial genes particularly in 12S rRNA gene. © 2015, Springer-Verlag Berlin Heidelberg

On the Schur multipliers of Lie superalgebras of maximal class

Let $L$ be a non-abelian nilpotent Lie superalgebra of dimensiom $(m|n)$. Nayak shows there is a non-negative $s(L)$ such that $s(L)=\frac{1}{2}(m+n-2)(m+n-1)+n+1-\dim{\mathcal{M}(L)}$. Here we intend that classify all non-abelian nilpotent Lie superalgebras, when $1\leq s(L)\leq 10$. Moreover, we classify the structure of all Lie superalgebras of dimension at most $5$ such that $\dim {L^2}=\dim {\mathcal{M}(L)}$

Subgroup Theorems for the B0~\tilde{B_0}-invariant of groups

U. Jezernik and P. Moravec have shown that if $G$ is a finite group with a subgroup $H$ of index $n$, then nth power of the Bogomolov multiplier of $G$, $\tilde{B_0}(G)^n$ is isomorphic to a subgroup of $\tilde{B_0}(H)$. In this paper we want to prove a similar result for the center by center by $w$ variety of groups, where $w$ is any outer commutator word

High resolution melting technique for molecular epidemiological studies of cystic echinococcosis: Differentiating G1, G3, and G6 genotypes of Echinococcus granulosus sensu lato

Reliable and rapid genotyping of large number of Echinococcus granulosus sensu lato isolates is crucial for understanding the epidemiology and transmission of cystic echinococcosis. We have developed a method for distinguishing and discriminating common genotypes of E. granulosus s.l. (G1, G3, and G6) in Iran. This method is based on polymerase chain reaction coupled with high resolution melting curve (HRM), ramping from 70 to 86 C with fluorescence data acquisition set at 0.1 C increments and continuous fluorescence monitoring. Consistency of this technique was assessed by inter- and intra-assays. Assessment of intra- and inter-assay variability showed low and acceptable coefficient of variations ranging from 0.09 to 0.17 %. Two hundred and eighty E. granulosus s.l. isolates from sheep, cattle, and camel were used to evaluate the applicability and accuracy of the method. The isolates were categorized as G1 (93, 94, and 25 %), G3 (7, 4, and 4 %), and G6 (0, 2, and 71 %) for sheep, cattle, and camel, respectively. HRM results were completely compatible with those obtained from sequencing and rostellar hook measurement. This method proved to be a valuable screening tool for large-scale molecular epidemiological studies. © 2013 Springer-Verlag Berlin Heidelberg

Molecular and morphological characterization of the tapeworm Taenia hydatigena (Pallas, 1766) in sheep from Iran

Although Taenia hydatigena is one of the most prevalent taeniid species of livestock, very little molecular genetic information exists for this parasite. Up to 100 sheep isolates of T. hydatigena were collected from 19 abattoirs located in the provinces of Tehran, Alborz and Kerman. A calibrated microscope was used to measure the larval rostellar hook lengths. Following DNA extraction, fragments of cytochrome c oxidase 1 (CO1) and 12S rRNA genes were amplified by the polymerase chain reaction method and the amplicons were subjected to sequencing. The mean total length of large and small hooks was 203.4 μm and 135.9 μm, respectively. Forty CO1 and 39 12S rRNA sequence haplotypes were obtained in the study. The levels of pairwise nucleotide variation between individual haplotypes of CO1 and 12S rRNA genes were determined to be between 0.3-3.4% and 0.2-2.1%, respectively. The overall nucleotide variation among all the CO1 haplotypes was 9.7%, and for all the 12S rRNA haplotypes it was 10.1%. A significant difference was observed between rostellar hook morphometry and both CO1 and 12S rRNA sequence variability. A significantly high level of genetic variation was observed in the present study. The results showed that the 12S rRNA gene is more variable than CO1. © 2013 Cambridge University Press

Relationship between biological egg characteristics and female brood fish in stellate sturgeon, Acipenser stellatus Pallas, 1771 (Pisces: Acipenseridae) in the Southeast Caspian Sea, Iran

Fecundity is one of the important indicators of reproduction biology in fishes especially in rearing and restocking management of endangered species such as sturgeon. The fecundity of stellate Sturgeon (Acipenser stellatus Pallas, 1771) was studied in 50 specimens captured by gill net (mesh size= 100 mm) and seine net in different fishing areas along the southeastern part of the Caspian Sea from October 2004 through June 2005.Some biological characteristics of eggs (including diameter, surface-to-volume ratio of egg) and of fish (including age, fork length, weight, fecundity and gonadosomatic indices) were determined, and the relationship between them was defined. The results obtained showed that eggs were in stage F5 of development, and mean fork length, weight, age, fecundity and gonadosomatic indices were 125.26±8.01 (cm), 10.3±1.97 (kg), 12±1.34 (years), 170730±43211.11 and 20.88±3.19 (%), respectively. Also, the average egg diameter and surface-to-volume ratio were 2.92±1.44 (mm) and 2.06±0.12 (mm-1), respectively. Positive correlation was detected between egg diameter and surface, and volume, while negative correlation was foun between egg diameter and surface-to-volume ratio. Weight, length and age have linear relationships with absolute fecundity and age was the best predicator of fecundity (r2= 0.848, F=27.962 and P value 0.003)

Survey of parasitic contamination of sewage sludges in northern Iran

The use of sewage sludge as fertilizer in agriculture is a convenient and economic solution, but it is essential to monitor presence of parasitic contamination. This study investigated parasites in sewage sludge of wastewater treatment plant in Gorgan, Iran. This descriptive-analytical study was performed on 18 sewage sludge samples collected from wastewater treatment plant of Gorgan within 6 months with three repeatitions per month. The samples were analyzed in the laboratory of School of Public Health at Golestan University of Medical Sciences. Analysis of parasites was done using Bailenger method of counting parasites in chamber of McMaster slides with volume of 0.3 ml. The results showed that majority of parasite eggs in the sewage sludge was related to nematodes. The dominant nematode eggs detected were related to Ancylostoma duodenale, Necator americanus and Enterobious vermicolaris. However, no parasite was found in dried sewage sludge. Considering the amount of parasite eggs in the returned sludge, it is necessary to modify the treatment process. However, it is permissible to use dried sewage sludge as agricultural fertilizer.Keywords: Parasite eggs, Protozoa, Sewage sludge, Ira