High Resolution Observations of the Massive Protostar in IRAS18566+0408

We report 3 mm continuum, CH3CN(5-4) and 13CS(2-1) line observations with CARMA, in conjunction with 6 and 1.3 cm continuum VLA data, and 12 and 25 micron broadband data from the Subaru Telescope toward the massive proto-star IRAS18566+0408. The VLA data resolve the ionized jet into 4 components aligned in the E-W direction. Radio components A, C, and D have flat cm SEDs indicative of optically thin emission from ionized gas, and component B has a spectral index alpha = 1.0, and a decreasing size with frequency proportional to frequency to the -0.5 power. Emission from the CARMA 3 mm continuum, and from the 13CS(2-1), and CH3CN(5-4) spectral lines is compact (i.e. < 6700 AU), and peaks near the position of VLA cm source, component B. Analysis of these lines indicates hot, and dense molecular gas, typical for HMCs. Our Subaru telescope observations detect a single compact source, coincident with radio component B, demonstrating that most of the energy in IRAS18566+0408 originates from a region of size < 2400 AU. We also present UKIRT near-infrared archival data for IRAS18566+0408 which show extended K-band emission along the jet direction. We detect an E-W velocity shift of about 10 km/sec over the HMC in the CH3CN lines possibly tracing the interface of the ionized jet with the surrounding core gas. Our data demonstrate the presence of an ionized jet at the base of the molecular outflow, and support the hypothesis that massive protostars with O-type luminosity form with a mechanism similar to lower mass stars

Evaluation of cell culture in microfluidic chips for application in monoclonal antibody production

Microfluidic chips are useful devices for cell culture that allow cell growth under highly controlled conditions, as is required for production of therapeutic recombinant proteins. To understand the optimal conditions for growth of cells amenable of recombinant protein expression in these devices,we culturedHEK-293T cells under different microfluidic experimental conditions. The cells were cultured in polymethyl methacrylate (PMMA) and polydi-methylsiloxane (PDMS)microdevices, in the absence or presence of the cell adhesion agent poly-D-lysine. Different microchannel geometries and thicknesses, as well as the influence of the flow rate have also been tested, showing their great influence in cell adhesion and growth. Results show that the presence of poly-D-lysine improves the adhesion and viability of the cells in continuous or discontinuous flow. Moreover, the optimal adhesion of cells was observed in the corners of themicrochannels, as well as in wide channels possibly due to the decrease in the flow rate in these areas. These studies provide insight into the optimal architecture of microchannels for long-term culture of adherent cells in order to use microfluidics devices as bioreactors for monoclonal antibodies production.Fil: Peñaherrera Pazmiño, Ana Belén. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Payés, Cristian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Sierra Rodero, Marina. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vega, M.. Universidad Tecnológica Nacional; ArgentinaFil: Rosero, G.. Universidad Tecnológica Nacional; Argentina. Universidad de Buenos Aires; ArgentinaFil: Lerner, Betiana. Universidad Tecnológica Nacional; Argentina. Universidad de Buenos Aires; ArgentinaFil: Helguera, Gustavo Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Pérez, M. S.. Universidad Tecnológica Nacional; Argentina. Universidad de Buenos Aires; Argentin

Weak and Compact Radio Emission in Early High-Mass Star Forming Regions: II. The Nature of the Radio Sources

In this study we analyze 70 radio continuum sources associated with dust clumps and considered to be candidates for the earliest stages of high-mass star formation. The detection of these sources was reported by Rosero et al. (2016), who found most of them to show weak (${\scriptstyle <}$1 mJy) and compact (${\scriptstyle <}\,$0.6$^{\prime \prime}$) radio emission. Herein, we used the observed parameters of these sources to investigate the origin of the radio continuum emission. We found that at least $\sim 30\%$ of these radio detections are most likely ionized jets associated with high-mass protostars, but for the most compact sources we cannot discard the scenario that they represent pressure-confined HII regions. This result is highly relevant for recent theoretical models based on core accretion that predict the first stages of ionization from high-mass stars to be in the form of jets. Additionally, we found that properties such as the radio luminosity as a function of the bolometric luminosity of ionized jets from low and high-mass stars are extremely well-correlated. Our data improve upon previous studies by providing further evidence of a common origin for jets independently of luminosity.Comment: Accepted for publication in the Ap

Weak and Compact Radio Emission in Early High-Mass Star Forming Regions: I. VLA Observations

We present a high sensitivity radio continuum survey at 6 and 1.3$\,$cm using the Karl G. Jansky Very Large Array towards a sample of 58 high-mass star forming regions. Our sample was chosen from dust clumps within infrared dark clouds with and without IR sources (CMC-IRs, CMCs, respectively), and hot molecular cores (HMCs), with no previous, or relatively weak radio continuum detection at the $1\,$mJy level. Due to the improvement in the continuum sensitivity of the VLA, this survey achieved map rms levels of $\sim$ 3-10 $\mu$Jy beam$^{-1}$ at sub-arcsecond angular resolution. We extracted 70 centimeter continuum sources associated with 1.2$\,$mm dust clumps. Most sources are weak, compact, and are prime candidates for high-mass protostars. Detection rates of radio sources associated with the mm dust clumps for CMCs, CMC-IRs and HMCs are 6$\%$, 53$\%$ and 100$\%$, respectively. This result is consistent with increasing high-mass star formation activity from CMCs to HMCs. The radio sources located within HMCs and CMC-IRs occur close to the dust clump centers with a median offset from it of 12,000$\,$AU and 4,000$\,$AU, respectively. We calculated 5 - 25$\,$GHz spectral indices using power law fits and obtain a median value of 0.5 (i.e., flux increasing with frequency), suggestive of thermal emission from ionized jets. In this paper we describe the sample, observations, and detections. The analysis and discussion will be presented in Paper II.Comment: Accepted for publication in the ApJ

Implementation of social and environmental standards: Continuity in use based on benefits and costs. A case study: the flower label program in Ecuador

135 p.In 1996 a German initiative created the Flower Label Program, with the main intention of improving the image of the floricultural sector in this country, and improving the social and environmental conditions in the developing countries in which flowers are produced. The scheme to be implemented by cut flower producers was seen by them to be an opportunity to reach a privileged position in this market, as the continued protests led by social and environmental organizations in Germany could create restrictions for the trade of non labelled flower in this market. Therefore flower growers saw in the label the possibility of improving access to the German market and gaining better prices while they accomplished what they consider to be part of their philosophy with respect to social and environmental concerns. However, after the label was implemented, the benefits obtained by flower growers were less than expected, creating the possibility that companies already working with the scheme could stop its use. This situation prompted the current study, which attempts to determine the principal factors influencing decisions regarding continued use of the label by flower producers. In order to accomplish this goal, the current qualitative study uses a conceptual framework based on the Technology Acceptance Model (TAM), in order to analyze the influence of different factors such as perceived benefits, perceived costs, external pressure and external variables in the final decision. Thus, the results revealed that the most important factor influencing continuity in the label’s use is the construct perceived costs which shows a direct influence in the final decision as well as an indirect effect through perceived benefits. Similarly, intangible economic benefits coming from enterprise image improvements were found to be crucial factors determining the label’s continuity in use, as well as the construct firm’s philosophy which influences indirectly the final decision by attenuating cost perceptions. Additionally, the construct enterprise ideas was also found to be an important determinant of the final decision, both directly and indirectly through the construct enterprise image

Best Practices and Methodologies for IT Project Portfolio Management

Abstract. In today's business environment, a key factor for decision making is the IT projects portfolio management (ITPPM). IT projects must be aligned with the objectives, goals and strategies, which is achieved through the appropriate application of a methodology for ITPPM. The experienced application of a methodology depends on the experience of the managers of ITPPM, considering that the application of methodologies generates best practices, it becomes necessary to study some of the more representative methodologies and obtain the best practices that allow novice administrators to initiate their portfolio management activities in information technology projects. In this article, three methodologies are studied, from which best practices are obtained, as results; a hierarchy of best practices is obtained so that project managers can be guided or introduced in their field

Broadband VLA Spectral Line Survey of a Sample of Ionized Jet Candidates

The study of the interaction between ionized jets, molecular outflows and their environments is critical to understanding high-mass star formation, especially because jets and outflows are thought to be key in the transfer of angular momentum outwards from accretion disks. We report a low-spectral resolution VLA survey for hydrogen radio recombination lines, OH, NH$_3$, and CH$_3$OH lines toward a sample of 58 high-mass star forming regions that contain numerous ionized jet candidates. The observations are from a survey designed to detect radio continuum; the novel aspect of this work is to search for spectral lines in broadband VLA data (we provide the script developed in this work to facilitate exploration of other datasets). We report detection of 25$\,$GHz CH$_3$OH transitions toward ten sources; five of them also show NH$_3$ emission. We found that most of the sources detected in CH$_3$OH and NH$_3$ have been classified as ionized jets or jet candidates and that the emission lines are coincident with, or very near ($\lesssim 0.1$ pc) these sources, hence, these molecular lines could be used as probes of the environment near the launching site of jets/outflows. No radio recombination lines were detected, but we found that the RMS noise of stacked spectra decreases following the radiometer equation. Therefore, detecting radio recombination lines in a sample of brighter free-free continuum sources should be possible. This work demonstrates the potential of broadband VLA continuum observations as low-resolution spectral line scans.Comment: 38 pages, 19 figures. Accepted for publication in The Astrophysical Journal Supplement Serie