Effects of 2500, 2900 and 3400 με on proliferation of primary osteoblast-like cells derived from female and male WT (A), <i>Lrp5</i>^−/− (B) and <i>LRP5</i>^G171V (C) mice.

<p>Changes in absolute number of cells between static and strain of both genotypes and genders are shown. Results are mean ± SEM of three independent experiments. Experiments were repeated 3 times. No significant differences at 2500 and 2900 με were observed. ***p<0.001 and *p<0.05 compared with the static control within the gender. (D) The effects of 3400 με on proliferation of primary osteoblast-like cells derived from female and male <i>LRP5</i>^G171V and <i>Lrp5</i>^−/− mice and their WT littermates. Percentage differences between static and strain of both genotypes and genders are shown. Results are the mean ± SEM of three independent experiments. Experiments were repeated 3 times. There were no significant differences between groups.</p

Percentage of apoptosis in osteoblast-like cells 48 hours after treatment with 0.1% (A), 2.5% (B) and 10% (C) serum.

<p>The TUNEL staining was used to determine the percentage of apoptotic cells in primary osteoblast-like cells derived from male and female WT_HBM, <i>Lrp5</i>^−/− and <i>LRP5</i>^G171V mice. Results are mean ± SEM of three independent experiments. Groups with the same letter are not significantly different.</p

Cell population doubling time of primary osteoblast-like cells derived from female and male <i>Lrp5</i><b>^−/−</b>, <i>LRP5</i><b>^G171V</b> and their WT littermates.

<p>Doubling time in days between 2 and 8 days of culture of primary osteoblast-like cells isolated from female and male <i>Lrp5</i>^−/−, <i>LRP5</i>^G171V and their WT littermates. Cell doubling time were calculated using GraphPad Prism v5.0 software for Windows (GraphPad Software Inc., San Diego, CA) by nonlinear regression (exponential growth equation) analysis.</p